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ß TCR Repertoire of Naive Mouse Splenocytes1
*
Unité de Biologie Moléculaire du Gène, Institut National de la Santé et de la Recherche Médicale, Unité 277, Institut Pasteur, Paris, France; and
Information Génétique et Structurale, EP 91, Centre National de la Recherche Scientifique, Institut de Biologie Structurale et Microbiologie, Marseille, France
The diversity of the T cell repertoire of mature T splenocytes is
generated, in the thymus, by pairing of 
and ß variable domains of
the ß TCR and by the rearrangements of various gene segments
encoding these domains. In the periphery, it results from competition
between various T cell subpopulations including recent thymic migrants
and long-lived T cells. Quantitative data on the actual size of the T
cell repertoire are lacking. Using PCR methods and extensive
sequencing, we have measured for the first time the size of the
TCR-ß repertoire of naive mouse T splenocytes. There are 5–8
x 105 different nucleotide sequences of BV chains in the
whole spleen of young adult mice. We have also determined the size of
the BV repertoire in a subpopulation of AV2+ T splenocytes,
which allows us to provide a minimum estimate of the ß repertoire.
We find that the mouse spleen harbors about 2 x 106
clones of about 10 cells each. This figure, although orders of
magnitude smaller than the maximum theoretical diversity (estimated up
to 1015), is still large enough to maintain a high
functional diversity.
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