| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
ß TCR Repertoire of Naive Mouse Splenocytes1
*
Unité de Biologie Moléculaire du Gène, Institut National de la Santé et de la Recherche Médicale, Unité 277, Institut Pasteur, Paris, France; and
Information Génétique et Structurale, EP 91, Centre National de la Recherche Scientifique, Institut de Biologie Structurale et Microbiologie, Marseille, France
The diversity of the T cell repertoire of mature T splenocytes is
generated, in the thymus, by pairing of 
and ß variable domains of
the ß TCR and by the rearrangements of various gene segments
encoding these domains. In the periphery, it results from competition
between various T cell subpopulations including recent thymic migrants
and long-lived T cells. Quantitative data on the actual size of the T
cell repertoire are lacking. Using PCR methods and extensive
sequencing, we have measured for the first time the size of the
TCR-ß repertoire of naive mouse T splenocytes. There are 5–8
x 105 different nucleotide sequences of BV chains in the
whole spleen of young adult mice. We have also determined the size of
the BV repertoire in a subpopulation of AV2+ T splenocytes,
which allows us to provide a minimum estimate of the ß repertoire.
We find that the mouse spleen harbors about 2 x 106
clones of about 10 cells each. This figure, although orders of
magnitude smaller than the maximum theoretical diversity (estimated up
to 1015), is still large enough to maintain a high
functional diversity.
This article has been cited by other articles:
|
|
 |
|
  V. Venturi, H. Y. Chin, D. A. Price, D. C. Douek, and M. P. Davenport The Role of Production Frequency in the Sharing of Simian Immunodeficiency Virus-Specific CD8+ TCRs between Macaques J. Immunol., August 15, 2008; 181(4): 2597 - 2609. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Yager, M. Ahmed, K. Lanzer, T. D. Randall, D. L. Woodland, and M. A. Blackman Age-associated decline in T cell repertoire diversity leads to holes in the repertoire and impaired immunity to influenza virus J. Exp. Med., March 17, 2008; 205(3): 711 - 723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Furmanski, C. Ferreira, I. Bartok, S. Dimakou, J. Rice, F. K. Stevenson, M. M. Millrain, E. Simpson, and J. Dyson Public T Cell Receptor -Chains Are Not Advantaged during Positive Selection J. Immunol., January 15, 2008; 180(2): 1029 - 1039. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Lim, B. Lemercier, X. Wertz, S. L. Pottier, F. Huetz, and P. Kourilsky Many human peripheral VH5-expressing IgM+ B cells display a unique heavy-chain rearrangement Int. Immunol., January 1, 2008; 20(1): 105 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Fazilleau, H. Bachelez, M.-L. Gougeon, and M. Viguier Cutting Edge: Size and Diversity of CD4+CD25high Foxp3+ Regulatory T Cell Repertoire in Humans: Evidence for Similarities and Partial Overlapping with CD4+CD25 T Cells J. Immunol., September 15, 2007; 179(6): 3412 - 3416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wong, R. Obst, M. Correia-Neves, G. Losyev, D. Mathis, and C. Benoist Adaptation of TCR Repertoires to Self-Peptides in Regulatory and Nonregulatory CD4+ T Cells J. Immunol., June 1, 2007; 178(11): 7032 - 7041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Purton, J. T. Tan, M. P. Rubinstein, D. M. Kim, J. Sprent, and C. D. Surh Antiviral CD4+ memory T cells are IL-15 dependent J. Exp. Med., April 16, 2007; 204(4): 951 - 961. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Venturi, K. Kedzierska, D. A. Price, P. C. Doherty, D. C. Douek, S. J. Turner, and M. P. Davenport Sharing of T cell receptors in antigen-specific responses is driven by convergent recombination PNAS, December 5, 2006; 103(49): 18691 - 18696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kedzierska, E. B. Day, J. Pi, S. B. Heard, P. C. Doherty, S. J. Turner, and S. Perlman Quantification of Repertoire Diversity of Influenza-Specific Epitopes with Predominant Public or Private TCR Usage J. Immunol., November 15, 2006; 177(10): 6705 - 6712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Hataye, J. J. Moon, A. Khoruts, C. Reilly, and M. K. Jenkins Naive and Memory CD4+ T Cell Survival Controlled by Clonal Abundance Science, April 7, 2006; 312(5770): 114 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Whitmire, N. Benning, and J. L. Whitton Precursor Frequency, Nonlinear Proliferation, and Functional Maturation of Virus-Specific CD4+ T Cells. J. Immunol., March 1, 2006; 176(5): 3028 - 3036. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-K. Kim, M. Cornberg, X. Z. Wang, H. D. Chen, L. K. Selin, and R. M. Welsh Private specificities of CD8 T cell responses control patterns of heterologous immunity J. Exp. Med., February 22, 2005; 201(4): 523 - 533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Fazilleau, J.-P. Cabaniols, F. Lemaitre, I. Motta, P. Kourilsky, and J. M. Kanellopoulos V{alpha} and V{beta} Public Repertoires Are Highly Conserved in Terminal Deoxynucleotidyl Transferase-Deficient Mice J. Immunol., January 1, 2005; 174(1): 345 - 355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Messaoudi, J. LeMaoult, J. A. Guevara-Patino, B. M. Metzner, and J. Nikolich-Zugich Age-related CD8 T Cell Clonal Expansions Constrict CD8 T Cell Repertoire and Have the Potential to Impair Immune Defense J. Exp. Med., November 15, 2004; 200(10): 1347 - 1358. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Anderson and K. L. Fritsche Dietary Polyunsaturated Fatty Acids Modulate In Vivo, Antigen-Driven CD4+ T-Cell Proliferation in Mice J. Nutr., August 1, 2004; 134(8): 1978 - 1983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Srinivasan, J. Foley, and S. J. McSorley Massive Number of Antigen-Specific CD4 T Cells during Vaccination with Live Attenuated Salmonella Causes Interclonal Competition J. Immunol., June 1, 2004; 172(11): 6884 - 6893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Joao, B. M. Ogle, C. Gay-Rabinstein, J. L. Platt, and M. Cascalho B Cell-Dependent TCR Diversification J. Immunol., April 15, 2004; 172(8): 4709 - 4716. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kedzierska, S. J. Turner, and P. C. Doherty Conserved T cell receptor usage in primary and recall responses to an immunodominant influenza virus nucleoprotein epitope PNAS, April 6, 2004; 101(14): 4942 - 4947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. L. Pewe, J. M. Netland, S. B. Heard, and S. Perlman Very Diverse CD8 T Cell Clonotypic Responses after Virus Infections J. Immunol., March 1, 2004; 172(5): 3151 - 3156. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. O. Schonland, J. K. Zimmer, C. M. Lopez-Benitez, T. Widmann, K. D. Ramin, J. J. Goronzy, and C. M. Weyand Homeostatic control of T-cell generation in neonates Blood, August 15, 2003; 102(4): 1428 - 1434. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. P. Badovinac, K. A. Nordyke Messingham, S. E. Hamilton, and J. T. Harty Regulation of CD8+ T Cells Undergoing Primary and Secondary Responses to Infection in the Same Host J. Immunol., May 15, 2003; 170(10): 4933 - 4942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hamrouni, A. Aublin, P. Guillaume, and J. L. Maryanski T Cell Receptor Gene Rearrangement Lineage Analysis Reveals Clues for the Origin of Highly Restricted Antigen-specific Repertoires J. Exp. Med., March 3, 2003; 197(5): 601 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Pasqual, M. Gallagher, C. Aude-Garcia, M. Loiodice, F. Thuderoz, J. Demongeot, R. Ceredig, P. N. Marche, and E. Jouvin-Marche Quantitative and Qualitative Changes in V-J {alpha} Rearrangements During Mouse Thymocytes Differentiation: Implication For a Limited T Cell Receptor {alpha} Chain Repertoire J. Exp. Med., November 4, 2002; 196(9): 1163 - 1174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. N. Blattman, R. Antia, D. J.D. Sourdive, X. Wang, S. M. Kaech, K. Murali-Krishna, J. D. Altman, and R. Ahmed Estimating the Precursor Frequency of Naive Antigen-specific CD8 T Cells J. Exp. Med., March 4, 2002; 195(5): 657 - 664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-P. Cabaniols, N. Fazilleau, A. Casrouge, P. Kourilsky, and J. M. Kanellopoulos Most {alpha}/{beta} T Cell Receptor Diversity Is Due to Terminal Deoxynucleotidyl Transferase J. Exp. Med., November 5, 2001; 194(9): 1385 - 1390. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Baron, I. McMorrow, D. H Sachs, and C. LeGuern Persistence of Dominant T Cell Clones in Accepted Solid Organ Transplants J. Immunol., October 15, 2001; 167(8): 4154 - 4160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Matsuda, L. Gapin, N. Fazilleau, K. Warren, O. V. Naidenko, and M. Kronenberg Natural killer T cells reactive to a single glycolipid exhibit a highly diverse T cell receptor beta repertoire and small clone size PNAS, October 5, 2001; (2001) 221445298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ronet, M. Mempel, N. Thieblemont, A. Lehuen, P. Kourilsky, and G. Gachelin Role of the Complementarity-Determining Region 3 (CDR3) of the TCR-{{beta}} Chains Associated with the V{{alpha}}14 Semi-Invariant TCR {{alpha}}-Chain in the Selection of CD4+ NK T Cells J. Immunol., February 1, 2001; 166(3): 1755 - 1762. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Laouini, A. Casrouge, S. Dalle, F. Lemonnier, P. Kourilsky, and J. Kanellopoulos V{beta} T Cell Repertoire of CD8+ Splenocytes Selected on Nonpolymorphic MHC Class I Molecules J. Immunol., December 1, 2000; 165(11): 6381 - 6386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Matsuda, L. Gapin, N. Fazilleau, K. Warren, O. V. Naidenko, and M. Kronenberg Natural killer T cells reactive to a single glycolipid exhibit a highly diverse T cell receptor beta repertoire and small clone size PNAS, October 23, 2001; 98(22): 12636 - 12641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yokosuka, K. Takase, M. Suzuki, Y. Nakagawa, S. Taki, H. Takahashi, T. Fujisawa, H. Arase, and T. Saito Predominant Role of T Cell Receptor (TCR)-{alpha} Chain in Forming Preimmune TCR Repertoire Revealed by Clonal TCR Reconstitution System J. Exp. Med., April 15, 2002; 195(8): 991 - 1001. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
