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The Journal of Immunology, 2000, 164: 5652-5658.
Copyright © 2000 by The American Association of Immunologists

Mouse CD8+ CD122+ T Cells with Intermediate TCR Increasing with Age Provide a Source of Early IFN-{gamma} Production

Eiji Takayama*, Shuhji Seki2,{dagger}, Takashi Ohkawa{dagger}, Katsunori Ami{dagger}, Yoshiko Habu{dagger}, Takanori Yamaguchi{dagger}, Takushi Tadakuma* and Hoshio Hiraide{dagger}

* Department of Parasitology, National Defense Medical College; and {dagger} Division of Basic Traumatology, National Defense Medical College Research Institute, Tokorozawa, Japan 4T. Ohkawa, S. Seki, H. Dobashi, Y. Koike, Y. Habu, K. Ami, H. Hiraide, and I. Sekine. Submitted for publication.

Although CD8+ IL-2Rß (CD122)+ T cells with intermediate TCR reportedly develop extrathymically, their functions still remain largely unknown. In the present study, we characterized the function of CD8+ CD122+ T cells with intermediate TCR of C57BL/6 mice. The proportion of CD8+ CD122+ T cells in splenocytes gradually increased with age, whereas CD8+ IL-2Rß-negative or -low (CD122-) T cells conversely decreased. The IFN-{gamma} production from splenocytes stimulated with immobilized anti-CD3 Ab in vitro increased with age, whereas the IL-4 production decreased. When sorted CD8+ CD122+ T cells were stimulated in vitro by the anti-CD3 Ab, they promptly produced a much larger amount of IFN-{gamma} than did CD8+ CD122- T cells or CD4+ T cells, whereas only CD4+ T cells produced IL-4. The depletion of CD8+ CD122+ T cells from whole splenocytes greatly decreased the CD3-stimulated IFN-{gamma} production and increased the IL-4 production, whereas the addition of sorted CD8+ CD122+ T cells to CD8+ CD122+ T cell-depleted splenocytes restored the IFN-{gamma} production and partially decreased IL-4 production. It is of interest that CD8+ CD122+ T cells stimulated CD4+ T cells to produce IFN-{gamma}. The CD3-stimulated IFN-{gamma} production from each T cell subset was augmented by macrophages. Furthermore, CD3-stimulated CD8+ CD122+ T cells produced an even greater amount of IFN-{gamma} than did liver NK1.1+ T cells and also showed antitumor cytotoxicity. These results show that CD8+ CD122+ T cells may thus be an important source of early IFN-{gamma} production and are suggested to be involved in the immunological changes with aging.




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