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*
Friedrich Miescher Institute, Basel, Switzerland;
Aaron Diamond AIDS Research Center, Rockefeller University, New York, NY 10016; and
Theoretical Biology and Biophysics, Los Alamos National Laboratory, Los Alamos, NM 87545
1 This work was supported by National Institutes of Health
Grants RR06555, AI28433 (to A. S. P.), and AI40387 (to
D. D. H.). S. B. gratefully acknowledges support from
the Novartis Research Foundation. Portions of this work were performed
under the auspices of the U.S. Department of Energy.
5-Bromo-2'-deoxyuridine (BrdU) is frequently used to measure the turnover of cell populations in vivo. However, due to a lack of detailed mathematical models that describe the uptake and loss of BrdU in dividing cell populations, assessments of cell turnover kinetics have been largely qualitative rather than quantitative. In this study, we develop a mathematical framework for the analysis of BrdU-labeling experiments. We derive analytical expressions for the fraction of labeled cells within cell populations that are growing, declining, or at equilibrium. Fitting the analytical functions to data allows us to quantify the rates of cell proliferation and cell loss, as well as the rate of cell input from a source. We illustrate this for the BrdU labeling of T lymphocytes of uninfected and SIV-infected rhesus macaques.
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