HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schubart, D. B. Articles by Matthias, P. Search for Related Content PubMed PubMed Citation Articles by Schubart, D. B. Articles by Matthias, P.

Cutting Edge: Lack of Peripheral B Cells and Severe Agammaglobulinemia in Mice Simultaneously Lacking Bruton’s Tyrosine Kinase and the B Cell-Specific Transcriptional Coactivator OBF-1

Daniel B. Schubart^1,*, Antonius Rolink, Karin Schubart^* and Patrick Matthias^2,*

^* Friedrich Miescher-Institute, Basel, Switzerland; and Basel Institute for Immunology, Basel, Switzerland

OBF-1 is a B cell-restricted transcriptional coactivator that is recruited to octamer-containing promoters by interacting with the POU domain of Oct-1 or Oct-2. We have shown earlier that mice lacking OBF-1 were dramatically impaired in their ability to mount humoral immune responses and did not develop germinal centers in the spleen; however, they had a largely normal B cell development in the bone marrow. In this study, we demonstrate that OBF-1-deficient mice also have an early defect in B cell development and show that OBF-1^-/- immature B cells are greatly impaired at the transition from the bone marrow to the spleen. In addition, when the OBF-1 mutation is combined to a mutation in the gene encoding Bruton’s tyrosine kinase, a striking phenotype is observed. These double-deficient animals lack peripheral B cells and have virtually no serum Igs, thus closely resembling human X chromosome-linked agammaglobulinemia.

This article has been cited by other articles:

				B. Bartholdy, C. Du Roure, A. Bordon, D. Emslie, L. M. Corcoran, and P. Matthias The Ets factor Spi-B is a direct critical target of the coactivator OBF-1 PNAS, August 1, 2006; 103(31): 11665 - 11670. [Abstract] [Full Text] [PDF]

				C. Brunner and T. Wirth Btk expression is controlled by Oct and BOB.1/OBF.1. Nucleic Acids Res., January 1, 2006; 34(6): 1807 - 1815. [Abstract] [Full Text] [PDF]

				C. Brunner, H. Laumen, P. J. Nielsen, N. Kraut, and T. Wirth Expression of the Aldehyde Dehydrogenase 2-like Gene Is Controlled by BOB.1/OBF.1 in B Lymphocytes J. Biol. Chem., November 14, 2003; 278(46): 45231 - 45239. [Abstract] [Full Text] [PDF]

				M. Jankovic and M. C. Nussenzweig OcaB regulates transitional B cell selection Int. Immunol., September 1, 2003; 15(9): 1099 - 1104. [Abstract] [Full Text] [PDF]

				J. Hess, P. J. Nielsen, K.-D. Fischer, H. Bujard, and T. Wirth The B Lymphocyte-Specific Coactivator BOB.1/OBF.1 Is Required at Multiple Stages of B-Cell Development Mol. Cell. Biol., March 1, 2001; 21(5): 1531 - 1539. [Abstract] [Full Text]

				U. Kim, C. S. Gunther, and R. G. Roeder Genetic Analyses of NFKB1 and OCA-B Function: Defects in B Cells, Serum IgM Level, and Antibody Responses in Nfkb1-/-Oca-b-/- Mice J. Immunol., December 15, 2000; 165(12): 6825 - 6832. [Abstract] [Full Text] [PDF]

				A. B. Satterthwaite, F. Willis, P. Kanchanastit, D. Fruman, L. C. Cantley, C. D. Helgason, R. K. Humphries, C. A. Lowell, M. Simon, M. Leitges, et al. A sensitized genetic system for the analysis of murine B lymphocyte signal transduction pathways dependent on Bruton's tyrosine kinase PNAS, June 6, 2000; 97(12): 6687 - 6692. [Abstract] [Full Text] [PDF]