The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vergnolle, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vergnolle, N.
The Journal of Immunology, 1999, 163: 5064-5069.
Copyright © 1999 by The American Association of Immunologists

Proteinase-Activated Receptor-2-Activating Peptides Induce Leukocyte Rolling, Adhesion, and Extravasation In Vivo1

Nathalie Vergnolle2

Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada

Proteinase-activated receptor 2 (PAR2) has been suggested to play a role in inflammatory reactions. Because leukocyte-endothelial cell interactions are critical events during inflammatory reactions, and because PAR2 is expressed both on endothelium and leukocytes, we have examined the effects of PAR2-activating peptides (PAR2-APs) on leukocyte rolling and adhesion in mesenteric venules and on leukocyte recruitment into the peritoneal cavity. Using intravital microscopy, leukocyte rolling, flux, and adhesion in rat mesenteric postcapillary venules were quantified. Topical addition of PAR2-APs (10 µM) for 1 min to the superfused venule induced a significant increase in leukocyte rolling and adherence. The increase in leukocyte adherence was not affected by pretreatment with a mast cell stabilizer (sodium cromoglycate) nor by prior degranulation of mast cells with compound 48/80. Nonetheless, both leukocyte rolling and adhesion were completely inhibited by pretreatment with a platelet-activating factor receptor antagonist (WEB 2086). Intraperitoneal injections of a selective PAR2-AP (SLIGRL-NH2) caused a significant increase in leukocyte migration into the peritoneal cavity. The effect of SLIGRL-NH2 on peritoneal leukocyte infiltration was completely inhibited by WEB 2086. These data suggest that PAR2 activation could contribute to several early events in the inflammatory reaction, including leukocyte rolling, adherence, and recruitment, by a mechanism dependent on platelet-activating factor release.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
P. Rallabhandi, Q. M. Nhu, V. Y. Toshchakov, W. Piao, A. E. Medvedev, M. D. Hollenberg, A. Fasano, and S. N. Vogel
Analysis of Proteinase-activated Receptor 2 and TLR4 Signal Transduction: A NOVEL PARADIGM FOR RECEPTOR COOPERATIVITY
J. Biol. Chem., September 5, 2008; 283(36): 24314 - 24325.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
E Hyun, P Andrade-Gordon, M Steinhoff, and N Vergnolle
Protease-activated receptor-2 activation: a major actor in intestinal inflammation
Gut, September 1, 2008; 57(9): 1222 - 1229.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
V. Shpacovitch, M. Feld, M. D. Hollenberg, T. A. Luger, and M. Steinhoff
Role of protease-activated receptors in inflammatory responses, innate and adaptive immunity
J. Leukoc. Biol., June 1, 2008; 83(6): 1309 - 1322.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. Jiang, A. Zatta, H. Kin, N. Wang, J. G. Reeves, J. Mykytenko, J. Deneve, Z.-Q. Zhao, R. A. Guyton, and J. Vinten-Johansen
PAR-2 activation at the time of reperfusion salvages myocardium via an ERK1/2 pathway in in vivo rat hearts
Am J Physiol Heart Circ Physiol, November 1, 2007; 293(5): H2845 - H2852.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
K. Shinagawa, J. A. Martin, V. A. Ploplis, and F. J. Castellino
Coagulation Factor Xa Modulates Airway Remodeling in a Murine Model of Asthma
Am. J. Respir. Crit. Care Med., January 15, 2007; 175(2): 136 - 143.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
R. Gatti, E. Andre, S. Amadesi, T. Q. Dinh, A. Fischer, N. W. Bunnett, S. Harrison, P. Geppetti, and M. Trevisani
Protease-activated receptor-2 activation exaggerates TRPV1-mediated cough in guinea pigs
J Appl Physiol, August 1, 2006; 101(2): 506 - 511.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
E. Camerer, I. Cornelissen, H. Kataoka, D. N. Duong, Y.-W. Zheng, and S. R. Coughlin
Roles of protease-activated receptors in a mouse model of endotoxemia
Blood, May 15, 2006; 107(10): 3912 - 3921.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. H. Cleator, W. Q. Zhu, D. E. Vaughan, and H. E. Hamm
Differential regulation of endothelial exocytosis of P-selectin and von Willebrand factor by protease-activated receptors and cAMP
Blood, April 1, 2006; 107(7): 2736 - 2744.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
U. Johansson, C. Lawson, M. Dabare, D. Syndercombe-Court, A. C. Newland, G. L. Howells, and M. G. Macey
Human peripheral blood monocytes express protease receptor-2 and respond to receptor activation by production of IL-6, IL-8, and IL-1{beta}
J. Leukoc. Biol., October 1, 2005; 78(4): 967 - 975.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Hezi-Yamit, P. W. Wong, N. Bien-Ly, L. G. Komuves, K. S. S. Prasad, D. R. Phillips, and U. Sinha
Synergistic induction of tissue factor by coagulation factor Xa and TNF: Evidence for involvement of negative regulatory signaling cascades
PNAS, August 23, 2005; 102(34): 12077 - 12082.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
X. Su, E. Camerer, J. R. Hamilton, S. R. Coughlin, and M. A. Matthay
Protease-Activated Receptor-2 Activation Induces Acute Lung Inflammation by Neuropeptide-Dependent Mechanisms
J. Immunol., August 15, 2005; 175(4): 2598 - 2605.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Dulon, D. Leduc, G. S. Cottrell, J. D'Alayer, K. K. Hansen, N. W. Bunnett, M. D. Hollenberg, D. Pidard, and M. Chignard
Pseudomonas aeruginosa Elastase Disables Proteinase-Activated Receptor 2 in Respiratory Epithelial Cells
Am. J. Respir. Cell Mol. Biol., May 1, 2005; 32(5): 411 - 419.
[Abstract] [Full Text] [PDF]

Home page
 Rheumatology (Oxford)Home page
M. D. Morgan, L. Harper, X. Lu, G. Nash, J. Williams, and C. O. S. Savage
Can neutrophils be manipulated in vivo?
Rheumatology, May 1, 2005; 44(5): 597 - 601.
[Full Text] [PDF]

Home page
 Endocr. Rev.Home page
M. Steinhoff, J. Buddenkotte, V. Shpacovitch, A. Rattenholl, C. Moormann, N. Vergnolle, T. A. Luger, and M. D. Hollenberg
Proteinase-Activated Receptors: Transducers of Proteinase-Mediated Signaling in Inflammation and Immune Response
Endocr. Rev., February 1, 2005; 26(1): 1 - 43.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
V. M. Shpacovitch, G. Varga, A. Strey, M. Gunzer, F. Mooren, J. Buddenkotte, N. Vergnolle, C. P. Sommerhoff, S. Grabbe, V. Gerke, et al.
Agonists of proteinase-activated receptor-2 modulate human neutrophil cytokine secretion, expression of cell adhesion molecules, and migration within 3-D collagen lattices
J. Leukoc. Biol., August 1, 2004; 76(2): 388 - 398.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
E. M. J. Peters, B. Handjiski, A. Kuhlmei, E. Hagen, H. Bielas, A. Braun, B. F. Klapp, R. Paus, and P. C. Arck
Neurogenic Inflammation in Stress-Induced Termination of Murine Hair Growth Is Promoted by Nerve Growth Factor
Am. J. Pathol., July 1, 2004; 165(1): 259 - 271.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
H. S. Rosario, S. W. Waldo, S. A. Becker, and G. W. Schmid-Schonbein
Pancreatic Trypsin Increases Matrix Metalloproteinase-9 Accumulation and Activation during Acute Intestinal Ischemia-Reperfusion in the Rat
Am. J. Pathol., May 1, 2004; 164(5): 1707 - 1716.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
V. S. OSSOVSKAYA and N. W. BUNNETT
Protease-Activated Receptors: Contribution to Physiology and Disease
Physiol Rev, April 1, 2004; 84(2): 579 - 621.
[Abstract] [Full Text] [PDF]

Home page
 CROBMHome page
F.T. Lundy and G.J. Linden
NEUROPEPTIDES AND NEUROGENIC MECHANISMS IN ORAL AND PERIODONTAL INFLAMMATION
Critical Reviews in Oral Biology & Medicine, March 1, 2004; 15(2): 82 - 98.
[Abstract] [Full Text] [PDF]

Home page
 Arch DermatolHome page
M. Steinhoff, S. Stander, S. Seeliger, J. C. Ansel, M. Schmelz, and T. Luger
Modern Aspects of Cutaneous Neurogenic Inflammation
Arch Dermatol, November 1, 2003; 139(11): 1479 - 1488.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
S. SEELIGER, C. K. DERIAN, N. VERGNOLLE, N. W. BUNNETT, R. NAWROTH, M. SCHMELZ, P.-Y. VON DER WEID, J. BUDDENKOTTE, C. SUNDERKOTTER, D. METZE, et al.
Proinflammatory role of proteinase-activated receptor-2 in humans and mice during cutaneous inflammation in vivo
FASEB J, October 1, 2003; 17(13): 1871 - 1885.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. C. Chin, N. Vergnolle, W. K. MacNaughton, J. L. Wallace, M. D. Hollenberg, and A. G. Buret
Proteinase-activated receptor 1 activation induces epithelial apoptosis and increases intestinal permeability
PNAS, September 16, 2003; 100(19): 11104 - 11109.
[Abstract] [Full Text] [PDF]

Home page
 PediatricsHome page
K. Cederqvist, C. Haglund, P. Heikkila, T. Sorsa, T. Tervahartiala, U.-H. Stenman, and S. Andersson
Pulmonary Trypsin-2 in the Development of Bronchopulmonary Dysplasia in Preterm Infants
Pediatrics, September 1, 2003; 112(3): 570 - 577.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
S. J. Bolton, C. A. McNulty, R. J. Thomas, C. R. A. Hewitt, and A. J. Wardlaw
Expression of and functional responses to protease-activated receptors on human eosinophils
J. Leukoc. Biol., July 1, 2003; 74(1): 60 - 68.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
C. D. Major, R. J. Santulli, C. K. Derian, and P. Andrade-Gordon
Extracellular Mediators in Atherosclerosis and Thrombosis: Lessons From Thrombin Receptor Knockout Mice
Arterioscler. Thromb. Vasc. Biol., June 1, 2003; 23(6): 931 - 939.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
R. C. Fields, J. G. Schoenecker, J. P. Hart, M. R. Hoffman, S. V. Pizzo, and J. H. Lawson
Protease-Activated Receptor-2 Signaling Triggers Dendritic Cell Development
Am. J. Pathol., June 1, 2003; 162(6): 1817 - 1822.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Dulon, C. Cande, N. W. Bunnett, M. D. Hollenberg, M. Chignard, and D. Pidard
Proteinase-Activated Receptor-2 and Human Lung Epithelial Cells: Disarming by Neutrophil Serine Proteinases
Am. J. Respir. Cell Mol. Biol., March 1, 2003; 28(3): 339 - 346.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. A. Boven, N. Vergnolle, S. D. Henry, C. Silva, Y. Imai, J. Holden, K. Warren, M. D. Hollenberg, and C. Power
Up-Regulation of Proteinase-Activated Receptor 1 Expression in Astrocytes During HIV Encephalitis
J. Immunol., March 1, 2003; 170(5): 2638 - 2646.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
R. Lang, P. I. Song, F. J. Legat, R. M. Lavker, B. Harten, H. Kalden, E. F. Grady, N. W. Bunnett, C. A. Armstrong, and J. C. Ansel
Human Corneal Epithelial Cells Express Functional PAR-1 and PAR-2
Invest. Ophthalmol. Vis. Sci., January 1, 2003; 44(1): 99 - 105.
[Abstract] [Full Text] [PDF]

Home page
 SEMIN CARDIOTHORAC VASC ANESTHHome page
R. C. Landis
Aprotinin: Antithrombotic and Vasoactive Mechanisms of Action
Seminars in Cardiothoracic and Vascular Anesthesia, December 1, 2002; 6(4): 307 - 312.
[Abstract] [PDF]

Home page
 J. Immunol.Home page
F. Schmidlin, S. Amadesi, K. Dabbagh, D. E. Lewis, P. Knott, N. W. Bunnett, P. R. Gater, P. Geppetti, C. Bertrand, and M. E. Stevens
Protease-Activated Receptor 2 Mediates Eosinophil Infiltration and Hyperreactivity in Allergic Inflammation of the Airway
J. Immunol., November 1, 2002; 169(9): 5315 - 5321.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
N. Cenac, A.-M. Coelho, C. Nguyen, S. Compton, P. Andrade-Gordon, W. K. MacNaughton, J. L. Wallace, M. D. Hollenberg, N. W. Bunnett, R. Garcia-Villar, et al.
Induction of Intestinal Inflammation in Mouse by Activation of Proteinase-Activated Receptor-2
Am. J. Pathol., November 1, 2002; 161(5): 1903 - 1915.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
A. F. Milia, M. B. Salis, T. Stacca, A. Pinna, P. Madeddu, M. Trevisani, P. Geppetti, and C. Emanueli
Protease-Activated Receptor-2 Stimulates Angiogenesis and Accelerates Hemodynamic Recovery in a Mouse Model of Hindlimb Ischemia
Circ. Res., August 23, 2002; 91(4): 346 - 352.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
Y. Wang, Y. Gu, and M. J. Lucas
Expression of Thrombin Receptors in Endothelial Cells and Neutrophils from Normal and Preeclamptic Pregnancies
J. Clin. Endocrinol. Metab., August 1, 2002; 87(8): 3728 - 3734.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
G. R. Stenton, O. Nohara, R. E. Dery, H. Vliagoftis, M. Gilchrist, A. Johri, J. L. Wallace, M. D. Hollenberg, R. Moqbel, and A. D. Befus
Proteinase-Activated Receptor (PAR)-1 and -2 Agonists Induce Mediator Release from Mast Cells by Pathways Distinct from PAR-1 and PAR-2
J. Pharmacol. Exp. Ther., August 1, 2002; 302(2): 466 - 474.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Vergnolle, C. K. Derian, M. R. D'Andrea, M. Steinhoff, and P. Andrade-Gordon
Characterization of Thrombin-Induced Leukocyte Rolling and Adherence: A Potential Proinflammatory Role for Proteinase-Activated Receptor-4
J. Immunol., August 1, 2002; 169(3): 1467 - 1473.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
A. Maree and D. Fitzgerald
PAR2 Is Partout and Now in the Heart
Circ. Res., March 8, 2002; 90(4): 366 - 368.
[Full Text] [PDF]

Home page
 Circ. Res.Home page
P. G. McLean, D. Aston, D. Sarkar, and A. Ahluwalia
Protease-Activated Receptor-2 Activation Causes EDHF-Like Coronary Vasodilation: Selective Preservation in Ischemia/Reperfusion Injury: Involvement of Lipoxygenase Products, VR1 Receptors, and C-Fibers
Circ. Res., March 8, 2002; 90(4): 465 - 472.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Fiorucci, A. Mencarelli, B. Palazzetti, E. Distrutti, N. Vergnolle, M. D. Hollenberg, J. L. Wallace, A. Morelli, and G. Cirino
Proteinase-activated receptor 2 is an anti-inflammatory signal for colonic lamina propria lymphocytes in a mouse model of colitis
PNAS, November 20, 2001; 98(24): 13936 - 13941.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
A. Alvarez and M.-J. Sanz
Reactive oxygen species mediate angiotensin II-induced leukocyte-endothelial cell interactions in vivo
J. Leukoc. Biol., August 1, 2001; 70(2): 199 - 206.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
S. R. Macfarlane, M. J. Seatter, T. Kanke, G. D. Hunter, and R. Plevin
Proteinase-Activated Receptors
Pharmacol. Rev., June 1, 2001; 53(2): 245 - 282.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. R. Lindner, M. L. Kahn, S. R. Coughlin, G. R. Sambrano, E. Schauble, D. Bernstein, D. Foy, A. Hafezi-Moghadam, and K. Ley
Delayed Onset of Inflammation in Protease-Activated Receptor-2-Deficient Mice
J. Immunol., December 1, 2000; 165(11): 6504 - 6510.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
H. Tapper and H. Herwald
Modulation of hemostatic mechanisms in bacterial infectious diseases
Blood, October 1, 2000; 96(7): 2329 - 2337.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. Camerer, W. Huang, and S. R. Coughlin
Tissue factor- and factor X-dependent activation of protease-activated receptor 2 by factor VIIa
PNAS, May 9, 2000; 97(10): 5255 - 5260.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. J. Compton, J. A. Cairns, K.-J. Palmer, B. Al-Ani, M. D. Hollenberg, and A. F. Walls
A Polymorphic Protease-activated Receptor 2 (PAR2) Displaying Reduced Sensitivity to Trypsin and Differential Responses to PAR Agonists
J. Biol. Chem., December 8, 2000; 275(50): 39207 - 39212.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. J. Ubl, Z. V. Grishina, T. K. Sukhomlin, T. Welte, F. Sedehizade, and G. Reiser
Human bronchial epithelial cells express PAR-2 with different sensitivity to thermolysin
Am J Physiol Lung Cell Mol Physiol, June 1, 2002; 282(6): L1339 - L1348.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
P. G. McLean, D. Aston, D. Sarkar, and A. Ahluwalia
Protease-Activated Receptor-2 Activation Causes EDHF-Like Coronary Vasodilation: Selective Preservation in Ischemia/Reperfusion Injury: Involvement of Lipoxygenase Products, VR1 Receptors, and C-Fibers
Circ. Res., March 8, 2002; 90(4): 465 - 472.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
J. R. Hamilton, A. G. Frauman, and T. M. Cocks
Increased Expression of Protease-Activated Receptor-2 (PAR2) and PAR4 in Human Coronary Artery by Inflammatory Stimuli Unveils Endothelium-Dependent Relaxations to PAR2 and PAR4 Agonists
Circ. Res., July 6, 2001; 89(1): 92 - 98.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.