HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yu, X.-Z. Articles by Anasetti, C. Search for Related Content PubMed PubMed Citation Articles by Yu, X.-Z. Articles by Anasetti, C.

Visualization, Fate, and Pathogenicity of Antigen-Specific CD8⁺ T Cells in the Graft-Versus-Host Reaction¹

Xue-Zhong Yu^*, Sasha Bidwell^*, Paul J. Martin^*, and Claudio Anasetti^2,*,

^* Division of Clinical Research, Fred Hutchinson Cancer Research Center, and Department of Medicine, Division of Oncology, University of Washington, Seattle, WA 98105

To follow the fate of alloreactive T cell effectors in graft-vs-host disease, L^d-specific CD8⁺ T cells from C57BL/6 2C TCR-transgenic donors were transplanted into sublethally irradiated (750 cGy) L^d+ or L^d- recipients. In L^d- C57BL/6 or (BALB/c-dm2 x C57BL/6)F₁ recipients, naive 2C T cells engrafted and survived long term, but did not acquire effector function. In L^d+ (BALB/c x C57BL/6)F₁ recipients, 2C T cells engrafted, expanded, became cytolytic, destroyed host B cells and double-positive thymocytes, and later disappeared. Despite marked damage to lymphoid and hemopoietic cells by 2C T cells, no significant pathology was detected in other organs, and recipients survived. L^d+ (BALB/c x C57BL/6)F₁ recipients died when LPS/endotoxin was administered on day 7 after cell transfer, while L^d- (BALB/c-dm2 x C57BL/6)F₁ recipients survived. Our findings show that under certain conditions, a CD8⁺ T cell population recognizing an extremely limited repertoire of Ags can initiate graft-vs-host disease.

This article has been cited by other articles:

				X.-Z. Yu, M. H. Albert, and C. Anasetti Alloantigen Affinity and CD4 Help Determine Severity of Graft-versus-Host Disease Mediated by CD8 Donor T Cells J. Immunol., March 15, 2006; 176(6): 3383 - 3390. [Abstract] [Full Text] [PDF]

				X.-Z. Yu, P. J. Martin, and C. Anasetti CD28 Signal Enhances Apoptosis of CD8 T Cells After Strong TCR Ligation J. Immunol., March 15, 2003; 170(6): 3002 - 3006. [Abstract] [Full Text] [PDF]

				L. Zhang, E. F. Lizzio, E. Gubina, T. Chen, H. Mostowski, and S. Kozlowski Organ-Specific Cytokine Polarization Induced by Adoptive Transfer of Transgenic T Cells J. Immunol., November 15, 2002; 169(10): 5514 - 5521. [Abstract] [Full Text] [PDF]

				M. Gonzalez, S. A. Quezada, B. R. Blazar, A. Panoskaltsis-Mortari, A. Y. Rudensky, and R. J. Noelle The Balance Between Donor T Cell Anergy and Suppression Versus Lethal Graft-Versus-Host Disease Is Determined by Host Conditioning J. Immunol., November 15, 2002; 169(10): 5581 - 5589. [Abstract] [Full Text] [PDF]

				X.-Z. Yu, S. J. Bidwell, P. J. Martin, and C. Anasetti Anti-CD3{{epsilon}} F(ab')2 Prevents Graft-Versus-Host Disease by Selectively Depleting Donor T Cells Activated by Recipient Alloantigens J. Immunol., May 1, 2001; 166(9): 5835 - 5839. [Abstract] [Full Text] [PDF]

				X.-Z. Yu, S. J. Bidwell, P. J. Martin, and C. Anasetti CD28-Specific Antibody Prevents Graft-Versus-Host Disease in Mice J. Immunol., May 1, 2000; 164(9): 4564 - 4568. [Abstract] [Full Text] [PDF]