| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
*
Biochemistry and
Medicine, Division of Infectious Diseases, Wake Forest University School of Medicine, Winston-Salem, NC 27157
The responses of human neutrophils (PMN) involve reorganization and
phosphorylation of cytoskeletal components. We investigated the
translocation of protein kinase C (PKC) isoforms to PMN cytoskeletal
(Triton-insoluble) fractions, in conjunction with activation of the
respiratory burst enzyme NADPH oxidase. In resting PMN, PKC-
(29%)
and small amounts of PKC-
(0.6%), but not PKC-ßII, were present
in cytoskeletal fractions. Upon stimulation with the PKC agonist PMA,
the levels of PKC-, PKC-ßII, and PKC- increased in the
cytoskeletal fraction, concomitant with a decrease in the
noncytoskeletal (Triton-soluble) fractions. PKC- maximally
associated with cytoskeletal fractions at 160 nM PMA and then declined,
while PKC- and PKC-ßII plateaued at 300 nM PMA. Translocation of
PKC- was maximal by 2 min and sustained for at least 10 min.
Translocation of PKC- and PKC-ßII was biphasic, plateauing at 2–3
min and then increasing up to 10 min. Under maximal stimulation
conditions, PKC isoforms were entirely cytoskeletal associated.
Translocation of the NADPH oxidase component
p47phox to the cytoskeletal fraction correlated
with translocation of PKC- and PKC-ßII, but not with translocation
of PKC-. Oxidase activity in cytoskeletal fractions paralleled
translocation of PKC-, PKC-ßII, and
p47phox. Stimulation with
1,2-dioctanoylglycerol resulted in little translocation of PKC isoforms
or p47phox, and in minimal oxidase activity. We
conclude that conventional PKC isoforms (PKC- and/or PKC-ßII) may
regulate PMA-stimulated cytoskeletal association and activation of
NADPH oxidase. PKC- may modulate other PMN responses that involve
cytoskeletal components.
This article has been cited by other articles:
|
|
 |
|
  N. Cheng, R. He, J. Tian, M. C. Dinauer, and R. D. Ye A Critical Role of Protein Kinase C{delta} Activation Loop Phosphorylation in Formyl-Methionyl-Leucyl-Phenylalanine-Induced Phosphorylation of p47phox and Rapid Activation of Nicotinamide Adenine Dinucleotide Phosphate Oxidase J. Immunol., December 1, 2007; 179(11): 7720 - 7728. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. R. Sheppard, M. R. Kelher, E. E. Moore, N. J. D. McLaughlin, A. Banerjee, and C. C. Silliman Structural organization of the neutrophil NADPH oxidase: phosphorylation and translocation during priming and activation J. Leukoc. Biol., November 1, 2005; 78(5): 1025 - 1042. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Tonks, J. Parton, A. J. Tonks, R. H. K. Morris, A. Finall, K. P. Jones, and S. K. Jackson Surfactant phospholipid DPPC downregulates monocyte respiratory burst via modulation of PKC Am J Physiol Lung Cell Mol Physiol, June 1, 2005; 288(6): L1070 - L1080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. He, M. Nanamori, H. Sang, H. Yin, M. C. Dinauer, and R. D. Ye Reconstitution of Chemotactic Peptide-Induced Nicotinamide Adenine Dinucleotide Phosphate (Reduced) Oxidase Activation in Transgenic COS-phox Cells J. Immunol., December 15, 2004; 173(12): 7462 - 7470. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Tuluc, A. Garcia, O. Bredetean, J. Meshki, and S. P. Kunapuli Primary granule release from human neutrophils is potentiated by soluble fibrinogen through a mechanism depending on multiple intracellular signaling pathways Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1264 - C1272. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tsunawaki, L. S. Yoshida, S. Nishida, T. Kobayashi, and T. Shimoyama Fungal Metabolite Gliotoxin Inhibits Assembly of the Human Respiratory Burst NADPH Oxidase Infect. Immun., June 1, 2004; 72(6): 3373 - 3382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. G. Shenoy, G. J. Gleich, and L. L. Thomas Eosinophil Major Basic Protein Stimulates Neutrophil Superoxide Production by a Class IA Phosphoinositide 3-Kinase and Protein Kinase C-{zeta}-Dependent Pathway J. Immunol., October 1, 2003; 171(7): 3734 - 3741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. R. Oliveira, R. Verlengia, C. R.O. Carvalho, L. R.G. Britto, R. Curi, and A. R. Carpinelli Pancreatic {beta}-Cells Express Phagocyte-Like NAD(P)H Oxidase Diabetes, June 1, 2003; 52(6): 1457 - 1463. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Todt, B. Hu, A. Punturieri, J. Sonstein, T. Polak, and J. L. Curtis Activation of Protein Kinase C beta II by the Stereo-specific Phosphatidylserine Receptor Is Required for Phagocytosis of Apoptotic Thymocytes by Resident Murine Tissue Macrophages J. Biol. Chem., September 20, 2002; 277(39): 35906 - 35914. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Oliver, R. T. Terry-Lorenzo, E. Elliott, W. A. C. Bloomer, S. Li, D. L. Brautigan, R. J. Colbran, and S. Shenolikar Targeting Protein Phosphatase 1 (PP1) to the Actin Cytoskeleton: the Neurabin I/PP1 Complex Regulates Cell Morphology Mol. Cell. Biol., July 1, 2002; 22(13): 4690 - 4701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Dellis, S. C. Gangloff, M. Paulais, D. Tondelier, J.-P. Rona, F. Brouillard, F. Bouteau, M. Guenounou, and J. Teulon Inhibition of the Calcium Release-activated Calcium (CRAC) Current in Jurkat T Cells by the HIV-1 Envelope Protein gp160 J. Biol. Chem., February 15, 2002; 277(8): 6044 - 6050. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M.-C. Dang, A. Fontayne, J. Hakim, J. El Benna, and A. Perianin Protein Kinase C {{zeta}} Phosphorylates a Subset of Selective Sites of the NADPH Oxidase Component p47phox and Participates in Formyl Peptide-Mediated Neutrophil Respiratory Burst J. Immunol., January 15, 2001; 166(2): 1206 - 1213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Regier, D. G. Greene, S. Sergeant, A. J. Jesaitis, and L. C. McPhail Phosphorylation of p22phox Is Mediated by Phospholipase D-dependent and -independent Mechanisms. CORRELATION OF NADPH OXIDASE ACTIVITY AND p22phox PHOSPHORYLATION J. Biol. Chem., September 8, 2000; 275(37): 28406 - 28412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Vancurova, V. Miskolci, and D. Davidson NF-kappa B Activation in Tumor Necrosis Factor alpha -stimulated Neutrophils Is Mediated by Protein Kinase Cdelta . CORRELATION TO NUCLEAR Ikappa Balpha J. Biol. Chem., June 1, 2001; 276(23): 19746 - 19752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. O'Flaherty, B. A. Chadwell, M. W. Kearns, S. Sergeant, and L. W. Daniel Protein Kinases C Translocation Responses to Low Concentrations of Arachidonic Acid J. Biol. Chem., June 29, 2001; 276(27): 24743 - 24750. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
