| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
,||
,§,||
*
Neuroscience Research Institute, State University of New York, Old Westbury, NY 11568;
Mind/Body Medical Institute, Beth Israel Deaconess Medical Center, Boston, MA 02215;
Unité de Neuroendocrinologie et Physiopathologie Neuronale, Institut National de la Santé et de la Recherche Médicale, U422, Lille, France;
§
Laboratoire d’Endocrinologie des Annélides, Centre National de la Recherche Scientifique, Université des Sciences et Technologies de Lille, Villeneuve d’Ascq, France;
¶
Institut National de la Santé et de la Recherche Médicale, U416, Institut Pasteur de Lille, Lille, France; and
||
Division of Cardiothoracic Surgery, Department of Surgery, State University of New York, Stony Brook, NY 11794
We tested the hypothesis that estrogen acutely stimulates
constitutive NO synthase (cNOS) activity in human peripheral monocytes
by acting on an estrogen surface receptor. NO release was measured in
real time with an amperometric probe. 17ß-estradiol exposure to
monocytes stimulated NO release within seconds in a
concentration-dependent manner, whereas 17
-estradiol had no effect.
17ß-estradiol conjugated to BSA (E2-BSA) also stimulated
NO release, suggesting mediation by a membrane surface receptor.
Tamoxifen, an estrogen receptor inhibitor, antagonized the action of
both 17ß-estradiol and E2-BSA, whereas ICI 182,780, a
selective inhibitor of the nuclear estrogen receptor, had no effect. We
further showed, using a dual emission microfluorometry in a
calcium-free medium, that the 17ß-estradiol-stimulated release of
monocyte NO was dependent on the initial stimulation of intracellular
calcium transients in a tamoxifen-sensitive process. Leeching out the
intracellular calcium stores abolished the effect of 17ß-estradiol on
NO release. RT-PCR analysis of RNA obtained from the cells revealed a
strong estrogen receptor- amplification signal and a weak ß
signal. Taken together, a physiological dose of estrogen acutely
stimulates NO release from human monocytes via the activation of an
estrogen surface receptor that is coupled to increases in intracellular
calcium.
This article has been cited by other articles:
|
|
 |
|
  Y. Osorio, D. L. Bonilla, A. G. Peniche, P. C. Melby, and B. L. Travi Pregnancy enhances the innate immune response in experimental cutaneous leishmaniasis through hormone-modulated nitric oxide production J. Leukoc. Biol., June 1, 2008; 83(6): 1413 - 1422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Subramanian and C. Shaha Up-Regulation of Bcl-2 through ERK Phosphorylation Is Associated with Human Macrophage Survival in an Estrogen Microenvironment J. Immunol., August 15, 2007; 179(4): 2330 - 2338. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. H. Straub The Complex Role of Estrogens in Inflammation Endocr. Rev., August 1, 2007; 28(5): 521 - 574. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bouman, M. J. Heineman, and M. M. Faas Sex hormones and the immune response in humans Hum. Reprod. Update, July 1, 2005; 11(4): 411 - 423. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Xia and T. L. Krukoff Estrogen Induces Nitric Oxide Production via Activation of Constitutive Nitric Oxide Synthases in Human Neuroblastoma Cells Endocrinology, October 1, 2004; 145(10): 4550 - 4557. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Willis, J.M. Morris, V. Danis, and E.D.M. Gallery Cytokine production by peripheral blood monocytes during the normal human ovulatory menstrual cycle Hum. Reprod., June 1, 2003; 18(6): 1173 - 1178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. B. Stefano, P. Cadet, K. Mantione, J. J. Cho, D. Jones, and W. Zhu Estrogen Signaling at the Cell Surface Coupled to Nitric Oxide Release in Mytilus edulis Nervous System Endocrinology, April 1, 2003; 144(4): 1234 - 1240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Alvarez, C. Hermenegildo, A. C. Issekutz, J. V. Esplugues, and M.-J. Sanz Estrogens Inhibit Angiotensin II-Induced Leukocyte-Endothelial Cell Interactions In Vivo via Rapid Endothelial Nitric Oxide Synthase and Cyclooxygenase Activation Circ. Res., December 13, 2002; 91(12): 1142 - 1150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Maccarrone, M. Bari, N. Battista, and A. Finazzi-Agro Estrogen stimulates arachidonoylethanolamide release from human endothelial cells and platelet activation Blood, December 1, 2002; 100(12): 4040 - 4048. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Vasudevan, S. Ogawa, and D. Pfaff Estrogen and Thyroid Hormone Receptor Interactions: Physiological Flexibility by Molecular Specificity Physiol Rev, October 1, 2002; 82(4): 923 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. McNeill, C. Zhang, F. Z. Stanczyk, S. P. Duckles, and D. N. Krause Estrogen Increases Endothelial Nitric Oxide Synthase via Estrogen Receptors in Rat Cerebral Blood Vessels: Effect Preserved After Concurrent Treatment With Medroxyprogesterone Acetate or Progesterone Stroke, June 1, 2002; 33(6): 1685 - 1691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Sakihama, S. Nakamura, and H. Yamasaki Nitric Oxide Production Mediated by Nitrate Reductase in the Green Alga Chlamydomonas reinhardtii: an Alternative NO Production Pathway in Photosynthetic Organisms Plant Cell Physiol., March 1, 2002; 43(3): 290 - 297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Pfeilschifter, R. Koditz, M. Pfohl, and H. Schatz Changes in Proinflammatory Cytokine Activity after Menopause Endocr. Rev., February 1, 2002; 23(1): 90 - 119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Vasudevan, L.-M. Kow, and D. W. Pfaff Early membrane estrogenic effects required for full expression of slower genomic actions in a nerve cell line PNAS, September 19, 2001; (2001) 221449798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. P. M. Benten, C. Stephan, M. Lieberherr, and F. Wunderlich Estradiol Signaling via Sequestrable Surface Receptors Endocrinology, April 1, 2001; 142(4): 1669 - 1677. [Abstract] [Full Text]
|
|
|
|
|
|
G. B. Stefano, P. Cadet, C. Breton, Y. Goumon, V. Prevot, J. P. Dessaint, J.-C. Beauvillain, A. S. Roumier, I. Welters, and M. Salzet Estradiol-stimulated nitric oxide release in human granulocytes is dependent on intracellular calcium transients: evidence of a cell surface estrogen receptor Blood, June 15, 2000; 95(12): 3951 - 3958. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kahlert, S. Nuedling, M. van Eickels, H. Vetter, R. Meyer, and C. Grohe Estrogen Receptor alpha Rapidly Activates the IGF-1 Receptor Pathway J. Biol. Chem., June 9, 2000; 275(24): 18447 - 18453. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Vasudevan, L.-M. Kow, and D. W. Pfaff Early membrane estrogenic effects required for full expression of slower genomic actions in a nerve cell line PNAS, October 9, 2001; 98(21): 12267 - 12271. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
