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(1,3)-Fucosyltransferase VII and (2,3)-Sialyltransferase IV Are Up-Regulated in Activated CD4 T Cells and Maintained After Their Differentiation into Th1 and Migration into Inflammatory Sites1
Section of Immunobiology, Yale University School of Medicine and Howard Hughes Medical Institute, New Haven, CT 06520
Activated Th1 CD4 T cells bind to P-selectin and migrate into
inflamed tissue, whereas Th2 cells do not. We show that
(1,3)-fucosyltransferase VII (FucT-VII) and
(2,3)-sialyltransferase IV (ST3GalIV), which are crucial for the
biosynthesis of functional P-selectin ligands, are absent in naive CD4
T cells, but are rapidly up-regulated upon activation. Th1 or Th2
differentiation in the presence of polarizing cytokines leads to
down-regulation of FucT-VII mRNA selectively in Th2 but not in Th1
cells. Influencing the differentiation by varying the priming dose of
antigenic peptide results in similar FucT-VII down-regulation only in
Ag-specific Th2 cells. ST3GalIV levels remain elevated. FucT-VII and
ST3GalIV mRNAs are also up-regulated by Th1 cells primed in vivo and
recruited into the lymph nodes draining delayed-type hypersensitivity
sites. We identify FucT-VII gene expression as a principal difference
between Th1 and Th2 cells, and underscore the importance of FucT-VII
and ST3GalIV expression for the biosynthesis of functional selectin
ligands.
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