The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wherry, E. J.
Right arrow Articles by Eisenlohr, L. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wherry, E. J.
Right arrow Articles by Eisenlohr, L. C.
The Journal of Immunology, 1999, 163: 3735-3745.
Copyright © 1999 by The American Association of Immunologists

The Induction of Virus-Specific CTL as a Function of Increasing Epitope Expression: Responses Rise Steadily Until Excessively High Levels of Epitope Are Attained1

E. John Wherry*, Kristin A. Puorro*, Angel Porgador{dagger} and Laurence C. Eisenlohr2,*

* Department of Microbiology and Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, PA 19107; and {dagger} Department of Microbiology and Immunology, Faculty Health Sciences, University of Ben-Gurion, Beer-Sheva, Israel

The role of epitope expression levels in CD8+ T cell priming has been controversial. Yet this parameter is of great importance in the design of rational approaches to optimize CTL responses to a variety of pathogens. In this paper we examine the influence of epitope production on CD8+ T cell priming by exploiting a system that allows a 200-fold range of cell surface epitope expression in vitro with a fixed dose of vaccinia virus. Our results demonstrate that, with the exception of a notable decline at the highest level of epitope, the magnitude of the responding CTL population generated in vivo following equivalent viral infections is essentially proportional to epitope density.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
G. Plesa, A. E. Snook, S. A. Waldman, and L. C. Eisenlohr
Derivation and Fluidity of Acutely Induced Dysfunctional CD8+ T Cells
J. Immunol., April 15, 2008; 180(8): 5300 - 5308.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. J. Smithey, S. Brandt, N. E. Freitag, D. E. Higgins, and H. G. A. Bouwer
Stimulation of Enhanced CD8 T Cell Responses Following Immunization with a Hyper-Antigen Secreting Intracytosolic Bacterial Pathogen
J. Immunol., March 1, 2008; 180(5): 3406 - 3416.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. S. Joshi and S. M. Kaech
Effector CD8 T Cell Development: A Balancing Act between Memory Cell Potential and Terminal Differentiation
J. Immunol., February 1, 2008; 180(3): 1309 - 1315.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Goldwich, S. S. C. Hahn, S. Schreiber, S. Meier, E. Kampgen, R. Wagner, M. B. Lutz, and U. Schubert
Targeting HIV-1 Gag into the Defective Ribosomal Product Pathway Enhances MHC Class I Antigen Presentation and CD8+ T Cell Activation
J. Immunol., January 1, 2008; 180(1): 372 - 382.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
G. Zelinskyy, S. Balkow, S. Schimmer, T. Werner, M. M. Simon, and U. Dittmer
The Level of Friend Retrovirus Replication Determines the Cytolytic Pathway of CD8+ T-Cell-Mediated Pathogen Control
J. Virol., November 1, 2007; 81(21): 11881 - 11890.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. S. Russell, M. Iskandar, O. L. Mykytczuk, J. H. E. Nash, L. Krishnan, and S. Sad
A Reduced Antigen Load In Vivo, Rather Than Weak Inflammation, Causes a Substantial Delay in CD8+ T Cell Priming against Mycobacterium bovis (Bacillus Calmette-Guerin)
J. Immunol., July 1, 2007; 179(1): 211 - 220.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S.-A. Younes, L. Trautmann, B. Yassine-Diab, L. H. Kalfayan, A.-E. Kernaleguen, T. O. Cameron, R. Boulassel, L. J. Stern, J.-P. Routy, Z. Grossman, et al.
The Duration of Exposure to HIV Modulates the Breadth and the Magnitude of HIV-Specific Memory CD4+ T Cells
J. Immunol., January 15, 2007; 178(2): 788 - 797.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
G. Plesa, P. M. McKenna, M. J. Schnell, and L. C. Eisenlohr
Immunogenicity of cytopathic and noncytopathic viral vectors.
J. Virol., July 1, 2006; 80(13): 6259 - 6266.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. Kotelkin, I. M. Belyakov, L. Yang, J. A. Berzofsky, P. L. Collins, and A. Bukreyev
The NS2 Protein of Human Respiratory Syncytial Virus Suppresses the Cytotoxic T-Cell Response as a Consequence of Suppressing the Type I Interferon Response.
J. Virol., June 1, 2006; 80(12): 5958 - 5967.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. B. Zook, M. T. Howard, G. Sinnathamby, J. F. Atkins, and L. C. Eisenlohr
Epitopes Derived by Incidental Translational Frameshifting Give Rise to a Protective CTL Response.
J. Immunol., June 1, 2006; 176(11): 6928 - 6934.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
B. B. Porter and J. T. Harty
The Onset of CD8+-T-Cell Contraction Is Influenced by the Peak of Listeria monocytogenes Infection and Antigen Display
Infect. Immun., March 1, 2006; 74(3): 1528 - 1536.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. J. Wherry, T. N. Golovina, S. E. Morrison, G. Sinnathamby, M. J. McElhaugh, D. C. Shockey, and L. C. Eisenlohr
Re-evaluating the Generation of a "Proteasome-Independent" MHC Class I-Restricted CD8 T Cell Epitope
J. Immunol., February 15, 2006; 176(4): 2249 - 2261.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Margalit, H. M. Sheikhet, Y. Carmi, D. Berko, E. Tzehoval, L. Eisenbach, and G. Gross
Induction of Antitumor Immunity by CTL Epitopes Genetically Linked to Membrane-Anchored {beta}2-Microglobulin
J. Immunol., January 1, 2006; 176(1): 217 - 224.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
P. M. Lavoie, A. R. Dumont, H. McGrath, A.-E. Kernaleguen, and R.-P. Sekaly
Delayed expansion of a restricted T cell repertoire by low-density TCR ligands
Int. Immunol., July 1, 2005; 17(7): 931 - 941.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
I. Pinchuk, B. C. Starcher, B. Livingston, A. Tvninnereim, S. Wu, E. Appella, J. Sidney, A. Sette, and B. Wizel
A CD8+ T Cell Heptaepitope Minigene Vaccine Induces Protective Immunity against Chlamydia pneumoniae
J. Immunol., May 1, 2005; 174(9): 5729 - 5739.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. Sadagopal, R. R. Amara, D. C. Montefiori, L. S. Wyatt, S. I. Staprans, N. L. Kozyr, H. M. McClure, B. Moss, and H. L. Robinson
Signature for Long-Term Vaccine-Mediated Control of a Simian and Human Immunodeficiency Virus 89.6P Challenge: Stable Low-Breadth and Low-Frequency T-Cell Response Capable of Coproducing Gamma Interferon and Interleukin-2
J. Virol., March 15, 2005; 79(6): 3243 - 3253.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. N. Golovina, S. E. Morrison, and L. C. Eisenlohr
The Impact of Misfolding versus Targeted Degradation on the Efficiency of the MHC Class I-Restricted Antigen Processing
J. Immunol., March 1, 2005; 174(5): 2763 - 2769.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. B. J. Wong, C. B. Buck, X. Shen, and R. F. Siliciano
An Evaluation of Enforced Rapid Proteasomal Degradation as a Means of Enhancing Vaccine-Induced CTL Responses
J. Immunol., September 1, 2004; 173(5): 3073 - 3083.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
W. N. Haining, D. G. Anderson, S. R. Little, M. S. von Berwelt-Baildon, A. A. Cardoso, P. Alves, K. Kosmatopoulos, L. M. Nadler, R. Langer, and D. S. Kohane
pH-Triggered Microparticles for Peptide Vaccination
J. Immunol., August 15, 2004; 173(4): 2578 - 2585.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Rollenhagen, M. Sorensen, K. Rizos, R. Hurvitz, and D. Bumann
Antigen selection based on expression levels during infection facilitates vaccine development for an intracellular pathogen
PNAS, June 8, 2004; 101(23): 8739 - 8744.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. R. Betts, D. A. Price, J. M. Brenchley, K. Lore, F. J. Guenaga, A. Smed-Sorensen, D. R. Ambrozak, S. A. Migueles, M. Connors, M. Roederer, et al.
The Functional Profile of Primary Human Antiviral CD8+ T Cell Effector Activity Is Dictated by Cognate Peptide Concentration
J. Immunol., May 15, 2004; 172(10): 6407 - 6417.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. Stebbing, B. Gazzard, S. Patterson, M. Bower, D. Perumal, M. Nelson, A. McMichael, G. Ogg, A. Epenetos, F. Gotch, et al.
Antibody-targeted MHC complex-directed expansion of HIV-1- and KSHV-specific CD8+ lymphocytes: a new approach to therapeutic vaccination
Blood, March 1, 2004; 103(5): 1791 - 1795.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
E. B. Walker, D. Haley, W. Miller, K. Floyd, K. P. Wisner, N. Sanjuan, H. Maecker, P. Romero, H.-M. Hu, W. G. Alvord, et al.
gp100209-2M Peptide Immunization of Human Lymphocyte Antigen-A2+ Stage I-III Melanoma Patients Induces Significant Increase in Antigen-Specific Effector and Long-Term Memory CD8+ T Cells
Clin. Cancer Res., January 15, 2004; 10(2): 668 - 680.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J.-W. Youn, S.-H. Park, J. H. Cho, and Y. C. Sung
Optimal Induction of T-Cell Responses against Hepatitis C Virus E2 by Antigen Engineering in DNA Immunization
J. Virol., November 1, 2003; 77(21): 11596 - 11602.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. S. Kim and S. Perlman
Protection Against CTL Escape and Clinical Disease in a Murine Model of Virus Persistence
J. Immunol., August 15, 2003; 171(4): 2006 - 2013.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C. L. Efferson, J. Schickli, B. K. Ko, K. Kawano, S. Mouzi, P. Palese, A. Garcia-Sastre, and C. G. Ioannides
Activation of Tumor Antigen-Specific Cytotoxic T Lymphocytes (CTLs) by Human Dendritic Cells Infected with an Attenuated Influenza A Virus Expressing a CTL Epitope Derived from the HER-2/neu Proto-Oncogene
J. Virol., July 1, 2003; 77(13): 7411 - 7424.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Tourdot and K. G. Gould
Competition Between MHC Class I Alleles for Cell Surface Expression Alters CTL Responses to Influenza A Virus
J. Immunol., November 15, 2002; 169(10): 5615 - 5621.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
V. Novitsky, H. Cao, N. Rybak, P. Gilbert, M. F. McLane, S. Gaolekwe, T. Peter, I. Thior, T. Ndung'u, R. Marlink, et al.
Magnitude and Frequency of Cytotoxic T-Lymphocyte Responses: Identification of Immunodominant Regions of Human Immunodeficiency Virus Type 1 Subtype C
J. Virol., September 11, 2002; 76(20): 10155 - 10168.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. J. Wherry, M. J. McElhaugh, and L. C. Eisenlohr
Generation of CD8+ T Cell Memory in Response to Low, High, and Excessive Levels of Epitope
J. Immunol., May 1, 2002; 168(9): 4455 - 4461.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. N. Golovina, E. J. Wherry, T. N. J. Bullock, and L. C. Eisenlohr
Efficient and Qualitatively Distinct MHC Class I-Restricted Presentation of Antigen Targeted to the Endoplasmic Reticulum
J. Immunol., March 15, 2002; 168(6): 2667 - 2675.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. N. J. Bullock, T. A. Colella, and V. H. Engelhard
The Density of Peptides Displayed by Dendritic Cells Affects Immune Responses to Human Tyrosinase and gp100 in HLA-A2 Transgenic Mice
J. Immunol., March 1, 2000; 164(5): 2354 - 2361.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.