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The Journal of Immunology, 1999, 163: 3735-3745.
Copyright © 1999 by The American Association of Immunologists

The Induction of Virus-Specific CTL as a Function of Increasing Epitope Expression: Responses Rise Steadily Until Excessively High Levels of Epitope Are Attained1

E. John Wherry*, Kristin A. Puorro*, Angel Porgador{dagger} and Laurence C. Eisenlohr2,*

* Department of Microbiology and Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, PA 19107; and {dagger} Department of Microbiology and Immunology, Faculty Health Sciences, University of Ben-Gurion, Beer-Sheva, Israel

The role of epitope expression levels in CD8+ T cell priming has been controversial. Yet this parameter is of great importance in the design of rational approaches to optimize CTL responses to a variety of pathogens. In this paper we examine the influence of epitope production on CD8+ T cell priming by exploiting a system that allows a 200-fold range of cell surface epitope expression in vitro with a fixed dose of vaccinia virus. Our results demonstrate that, with the exception of a notable decline at the highest level of epitope, the magnitude of the responding CTL population generated in vivo following equivalent viral infections is essentially proportional to epitope density.




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