HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Etienne, S. Articles by Couraud, P.-O. Search for Related Content PubMed PubMed Citation Articles by Etienne, S. Articles by Couraud, P.-O.

MHC Class II Engagement in Brain Endothelial Cells Induces Protein Kinase A-Dependent IL-6 Secretion and Phosphorylation of cAMP Response Element-Binding Protein¹

Sandrine Etienne^2,*, Sandrine Bourdoulous^*, A. Donny Strosberg^* and Pierre-Olivier Couraud^*,

^* Laboratoire d’Immuno-Pharmacologie Moléculaire, Institut Cochin de Génétique Moléculaire, Centre National de la Recherche Scientifique, Unité Propre de Recherche 0415, Université Paris VII, Paris, France; and Neurotech SA, Evry, France

Activated endothelial cells can directly participate in immune responses by interacting with immunocompetent cells via class II MHC proteins. We show here that, after induction of MHC class II molecule expression by IFN-, rat brain endothelial cells responded to MHC class II ligands, anti-MHC class II Abs, or superantigens by expression of IL-6 transcript and IL-6 secretion. This response was not affected by protein kinase C depletion but was mimicked by the cAMP-elevating agent forskolin and completely blocked by H89, an inhibitor of cAMP-dependent protein kinase (PKA). Involvement of a cAMP/PKA signaling pathway in response to MHC class II ligands was further demonstrated by measure of a dose-dependent increase in cAMP level and phosphorylation of the transcription factor cAMP response element-binding protein (CREB). Our results indicate that MHC class II engagement in brain endothelial cells is directly coupled to IL-6 production via a cAMP/PKA-dependent intracellular pathway.

This article has been cited by other articles:

				N J Abbott, L L. Mendonca, and D E. Dolman The blood-brain barrier in systemic lupus erythematosus Lupus, December 1, 2003; 12(12): 908 - 915. [Abstract] [PDF]

				H.-C. Cheng, H.-M. Shih, and Y. Chern Essential Role of cAMP-response Element-binding Protein Activation by A2A Adenosine Receptors in Rescuing the Nerve Growth Factor-induced Neurite Outgrowth Impaired by Blockage of the MAPK Cascade J. Biol. Chem., September 6, 2002; 277(37): 33930 - 33942. [Abstract] [Full Text] [PDF]

				J. D. van Buul, C. Voermans, V. van den Berg, E. C. Anthony, F. P. J. Mul, S. van Wetering, C. E. van der Schoot, and P. L. Hordijk Migration of Human Hematopoietic Progenitor Cells Across Bone Marrow Endothelium Is Regulated by Vascular Endothelial Cadherin J. Immunol., January 15, 2002; 168(2): 588 - 596. [Abstract] [Full Text] [PDF]

				A. J. Ammit, R. K. Hoffman, Y. Amrani, A. L. Lazaar, D. W. P. Hay, T. J. Torphy, R. B. Penn, and R. A. Panettieri Jr. Tumor Necrosis Factor-alpha -Induced Secretion of RANTES and Interleukin-6 from Human Airway Smooth-Muscle Cells . Modulation by Cyclic Adenosine Monophosphate Am. J. Respir. Cell Mol. Biol., December 1, 2000; 23(6): 794 - 802. [Abstract] [Full Text]

				S. Etienne-Manneville, J.-B. Manneville, P. Adamson, B. Wilbourn, J. Greenwood, and P.-O. Couraud ICAM-1-Coupled Cytoskeletal Rearrangements and Transendothelial Lymphocyte Migration Involve Intracellular Calcium Signaling in Brain Endothelial Cell Lines J. Immunol., September 15, 2000; 165(6): 3375 - 3383. [Abstract] [Full Text] [PDF]