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Expression of Stromal Cell-Derived Factor-1/Pre-B Cell Growth-Stimulating Factor Receptor, CXC Chemokine Receptor 4, on CD34⁺ Human Bone Marrow Cells Is a Phenotypic Alteration for Committed Lymphoid Progenitors¹

Takefumi Ishii^*, Masamichi Nishihara^*, Feng Ma, Yasuhiro Ebihara, Kohichiro Tsuji, Shigetaka Asano, Tatsutoshi Nakahata and Taira Maekawa^2,*

Departments of ^* Transfusion Medicine and Cell Therapy, Clinical Oncology, and Pathological Pharmacology, The Institute of Medical Science, University of Tokyo, Tokyo, Japan

We found that the stromal cell-derived factor-1/pre-B cell growth-stimulating factor receptor, CXC chemokine receptor 4 (CXCR4), is expressed on human CD34⁺ bone marrow (BM) cells. Stringently FACS-sorted CD34⁺CXCR4⁺ BM cells completely lack myeloid, erythroid, megakaryocytic, and mixed colony-forming potential (myeloid progenitors), but give rise to B and T lymphoid progenitors, whereas CD34⁺CXCR4^- BM cells can generate colonies formed by myeloid progenitors and can also develop into these lymphoid progenitors. Therefore, expression of CXCR4 on CD34⁺ BM cells can allow lymphoid progenitors to be discriminated from myeloid progenitors. Because CD34⁺CXCR4⁺ cells are differentiated from CD34⁺CXCR4^- cells, multipotential progenitors located in the BM are likely to be negative for CXCR4 expression. CXCR4 seems to be expressed earlier than the IL-7R and terminal deoxynucleotidyl transferase during early lymphohemopoiesis. These results suggest that the expression of CXCR4 on CD34⁺ BM cells is one of the phenotypic alterations for committed lymphoid progenitors.

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