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*
Department of Pathology, Roger Williams Medical Center, Boston University School of Medicine, Boston, MA 02118; and
Lifespan Health Care System, Brown University School of Medicine, Providence, RI 02908
Normal mature quiescent human B lymphocytes, isolated as a function
of buoyant density, require activation for up-regulation of IL-13R
constituents. Cell activation through a combination of surface Ig and
CD40 receptor ligation leads to the most substantial message production
for IL-13R
1. Functional consequences of this receptor variation, in
initially quiescent cells, includes demonstrable effects on cellular
proliferation in response to ligand exposure. Variations in the method
of surface activation, with particular emphasis on the CD40 receptor,
reveals that immobilized CD40 ligand may be sufficient, in and of
itself, to up-regulate IL-13R1, which may bear significance for
B-lymphocyte bystander proliferation. Regulation of the IL-13R1
protein and message also differs as a function of cellular phenotype.
Although values are greater in memory than naive B cells, as they are
initially isolated from extirpated tonsils, variations in the magnitude
of message and protein, as a function of surface stimulation, are more
substantial in the naive subset. The magnitude of variation in message
production in naive cells is associated with a more vigorous
proliferative response to IL-13 than seen in memory lymphocytes. The
cellular response to IL-13, as a function of activation and phenotype,
is the converse of that demonstrated for IL-2. Evaluation of
proliferation, receptor message, ligand binding protein production, and
the response to putatively synergistic cytokines reveals that IL-2 is
the predominant lymphokine utilized by memory cells. This is in
contradistinction to IL-13, which along with IL-4, are the predominant
moieties for naive lymphocytes.
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