| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
- Dendritic Cells to Enhance Presentation of a Tumor Peptide In Vivo1
Departments of
*
Experimental Medicine and
Pharmacology, University of Perugia, Perugia, Italy
Previous work has shown that a significant proportion of murine
splenic dendritic cells (DC) express a high affinity receptor for
IL-12, thus accounting for the adjuvanticity of the cytokine when DBA/2
mice are transferred with syngeneic DC exposed in vitro to rIL-12 and
an otherwise poorly immunogenic tumor peptide. In DBA/2 mice, splenic
DC consist of 90–95% CD8- and 5–10% CD8+
cells. To detect any difference in IL-12 responsiveness among
phenotypically distinct DC subtypes, enriched CD8- (>99%
pure) and CD8+ (
80% pure) populations of DC from DBA/2
spleens were assayed for APC function in vivo following exposure to
rIL-12 and tumor peptide in vitro. Unlike unfractionated DC, the
CD8- fraction was capable of effective presentation of the
peptide even when the cells had not been pretreated with IL-12 before
peptide pulsing. The addition of as few as 3% CD8+ cells
during pulsing blocked in vivo priming by the CD8-
fraction. However, pretreatment of CD8- DC with IL-12
before cell mixing and peptide pulsing ablated the inhibitory effect of
the CD8+ fraction. CD8-, but not
CD8+, DC showed significant message expression for the ß1
and ß2 subunits of the IL-12 receptor. These data suggest that a
minority population of CD8+ DC, which appeared to secrete
IL-10 in vitro, negatively regulates the induction of T cell reactivity
by peptide-loaded CD8- DC in DBA/2 mice. However, the
CD8- fraction can be primed by IL-12 to overcome the
inhibitory effect of the CD8+
subtype.
This article has been cited by other articles:
|
|
 |
|
  F. v. Rhee Idiotype Vaccination Strategies in Myeloma: How to Overcome a Dysfunctional Immune System Clin. Cancer Res., March 1, 2007; 13(5): 1353 - 1355. [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Belladonna, U. Grohmann, P. Guidetti, C. Volpi, R. Bianchi, M. C. Fioretti, R. Schwarcz, F. Fallarino, and P. Puccetti Kynurenine Pathway Enzymes in Dendritic Cells Initiate Tolerogenesis in the Absence of Functional IDO J. Immunol., July 1, 2006; 177(1): 130 - 137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhang, H. Huang, J. Yuan, D. Sun, W.-S. Hou, J. Gordon, and J. Xiang CD4-8- Dendritic Cells Prime CD4+ T Regulatory 1 Cells to Suppress Antitumor Immunity J. Immunol., September 1, 2005; 175(5): 2931 - 2937. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Gordon, F. Li, A. Nayyar, J. Xiang, and X. Zhang CD8{alpha}+, but Not CD8{alpha}-, Dendritic Cells Tolerize Th2 Responses via Contact-Dependent and -Independent Mechanisms, and Reverse Airway Hyperresponsiveness, Th2, and Eosinophil Responses in a Mouse Model of Asthma J. Immunol., August 1, 2005; 175(3): 1516 - 1522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Orabona, M. L. Belladonna, C. Vacca, R. Bianchi, F. Fallarino, C. Volpi, S. Gizzi, M. C. Fioretti, U. Grohmann, and P. Puccetti Cutting Edge: Silencing Suppressor of Cytokine Signaling 3 Expression in Dendritic Cells Turns CD28-Ig from Immune Adjuvant to Suppressant J. Immunol., June 1, 2005; 174(11): 6582 - 6586. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Fallarino, R. Bianchi, C. Orabona, C. Vacca, M. L. Belladonna, M. C. Fioretti, D. V. Serreze, U. Grohmann, and P. Puccetti CTLA-4-Ig Activates Forkhead Transcription Factors and Protects Dendritic Cells from Oxidative Stress in Nonobese Diabetic Mice J. Exp. Med., October 18, 2004; 200(8): 1051 - 1062. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Peng, S. F. Hussain, and Y. Paterson The Ability of Two Listeria monocytogenes Vaccines Targeting Human Papillomavirus-16 E7 to Induce an Antitumor Response Correlates with Myeloid Dendritic Cell Function J. Immunol., May 15, 2004; 172(10): 6030 - 6038. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. L. Colvin, A. E. Morelli, A. J. Logar, A. H. Lau, and A. W. Thomson Comparative evaluation of CC chemokine-induced migration of murine CD8{alpha}+ and CD8{alpha}- dendritic cells and their in vivo trafficking J. Leukoc. Biol., February 1, 2004; 75(2): 275 - 285. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Grohmann, R. Bianchi, C. Orabona, F. Fallarino, C. Vacca, A. Micheletti, M. C. Fioretti, and P. Puccetti Functional Plasticity of Dendritic Cell Subsets as Mediated by CD40 Versus B7 Activation J. Immunol., September 1, 2003; 171(5): 2581 - 2587. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Tsung, J. P. Dolan, Y. L. Tsung, and J. A. Norton Macrophages as Effector Cells in Interleukin 12-induced T Cell-dependent Tumor Rejection Cancer Res., September 1, 2002; 62(17): 5069 - 5075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Fallarino, U. Grohmann, C. Vacca, R. Bianchi, M. C. Fioretti, and P. Puccetti CD40 Ligand and CTLA-4 Are Reciprocally Regulated in the Th1 Cell Proliferative Response Sustained by CD8+ Dendritic Cells J. Immunol., August 1, 2002; 169(3): 1182 - 1188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Belladonna, J.-C. Renauld, R. Bianchi, C. Vacca, F. Fallarino, C. Orabona, M. C. Fioretti, U. Grohmann, and P. Puccetti IL-23 and IL-12 Have Overlapping, but Distinct, Effects on Murine Dendritic Cells J. Immunol., June 1, 2002; 168(11): 5448 - 5454. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Teshima, P. Reddy, K. P. Lowler, M. A. KuKuruga, C. Liu, K. R. Cooke, and J. L. M. Ferrara Flt3 ligand therapy for recipients of allogeneic bone marrow transplants expands host CD8alpha + dendritic cells and reduces experimental acute graft-versus-host disease Blood, March 1, 2002; 99(5): 1825 - 1832. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Lipscomb and B. J. Masten Dendritic Cells: Immune Regulators in Health and Disease Physiol Rev, January 1, 2002; 82(1): 97 - 130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. O'Connell, W. Li, Z. Wang, S. M. Specht, A. J. Logar, and A. W. Thomson Immature and Mature CD8{alpha}+ Dendritic Cells Prolong the Survival of Vascularized Heart Allografts J. Immunol., January 1, 2002; 168(1): 143 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Lee, F. Wang, J. Kuniyoshi, V. Rubio, T. Stuges, S. Groshen, C. Gee, R. Lau, G. Jeffery, K. Margolin, et al. Effects of Interleukin-12 on the Immune Response to a Multipeptide Vaccine for Resected Metastatic Melanoma J. Clin. Oncol., September 15, 2001; 19(18): 3836 - 3847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Grohmann, F. Fallarino, R. Bianchi, M. L. Belladonna, C. Vacca, C. Orabona, C. Uyttenhove, M. C. Fioretti, and P. Puccetti IL-6 Inhibits the Tolerogenic Function of CD8{{alpha}}+ Dendritic Cells Expressing Indoleamine 2,3-Dioxygenase J. Immunol., July 15, 2001; 167(2): 708 - 714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Grohmann, M. L. Belladonna, C. Vacca, R. Bianchi, F. Fallarino, C. Orabona, M. C. Fioretti, and P. Puccetti Positive Regulatory Role of IL-12 in Macrophages and Modulation by IFN-{{gamma}} J. Immunol., July 1, 2001; 167(1): 221 - 227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hochrein, K. Shortman, D. Vremec, B. Scott, P. Hertzog, and M. O'Keeffe Differential Production of IL-12, IFN-{{alpha}}, and IFN-{{gamma}} by Mouse Dendritic Cell Subsets J. Immunol., May 1, 2001; 166(9): 5448 - 5455. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Grohmann, F. Fallarino, S. Silla, R. Bianchi, M. L. Belladonna, C. Vacca, A. Micheletti, M. C. Fioretti, and P. Puccetti CD40 Ligation Ablates the Tolerogenic Potential of Lymphoid Dendritic Cells J. Immunol., January 1, 2001; 166(1): 277 - 283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Fallarino, U. Grohmann, R. Bianchi, C. Vacca, M. C. Fioretti, and P. Puccetti Th1 and Th2 Cell Clones to a Poorly Immunogenic Tumor Antigen Initiate CD8+ T Cell-Dependent Tumor Eradication In Vivo J. Immunol., November 15, 2000; 165(10): 5495 - 5501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Martin, G. M. del Hoyo, F. Anjuere, S. R. Ruiz, C. F. Arias, A. R. Marin, and C. Ardavin Concept of lymphoid versus myeloid dendritic cell lineages revisited: both CD8alpha - and CD8alpha + dendritic cells are generated from CD4low lymphoid-committed precursors Blood, October 1, 2000; 96(7): 2511 - 2519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Grohmann, R. Bianchi, M. L. Belladonna, S. Silla, F. Fallarino, M. C. Fioretti, and P. Puccetti IFN-{gamma} Inhibits Presentation of a Tumor/Self Peptide by CD8{alpha}- Dendritic Cells Via Potentiation of the CD8{alpha}+ Subset J. Immunol., August 1, 2000; 165(3): 1357 - 1363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Nagayama, K. Sato, H. Kawasaki, M. Enomoto, C. Morimoto, K. Tadokoro, T. Juji, S. Asano, and T. A. Takahashi IL-12 Responsiveness and Expression of IL-12 Receptor in Human Peripheral Blood Monocyte-Derived Dendritic Cells J. Immunol., July 1, 2000; 165(1): 59 - 66. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. F. d'Ostiani, G. Del Sero, A. Bacci, C. Montagnoli, A. Spreca, A. Mencacci, P. Ricciardi-Castagnoli, and L. Romani Dendritic Cells Discriminate between Yeasts and Hyphae of the Fungus Candida albicans: Implications for Initiation of T Helper Cell Immunity In Vitro and In Vivo J. Exp. Med., May 8, 2000; 191(10): 1661 - 1674. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Grohmann, J. Van Snick, F. Campanile, S. Silla, A. Giampietri, C. Vacca, J.-C. Renauld, M. C. Fioretti, and P. Puccetti IL-9 Protects Mice from Gram-Negative Bacterial Shock: Suppression of TNF-{alpha}, IL-12, and IFN-{gamma}, and Induction of IL-10 J. Immunol., April 15, 2000; 164(8): 4197 - 4203. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
