| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Laboratory of Immunology, Istituto Dermopatico dell’Immacolata, Instituto di Ricovero e Cura a Carattere Scientifico, Rome, Italy;
Section of Microbiology, Department of Molecular Biology, University of Siena, Siena, Italy; and
Department of Biotechnology and Bioscience, University of Milan-Bicocca, Milan, Italy
Recombinant Streptococcus gordonii expressing on the
surface the C-fragment of tetanus toxin was tested as an Ag delivery
system for human monocyte-derived dendritic cells (DCs). DCs incubated
with recombinant S. gordonii were much more efficient
than DCs pulsed with soluble C-fragment of tetanus toxin at stimulating
specific CD4+ T cells as determined by cell proliferation
and IFN-
release. Compared with DCs treated with soluble Ag, DCs fed
with recombinant bacteria required 102- to
103-fold less Ag and were at least 102 times
more effective on a per-cell basis for activating specific T cells.
S. gordonii was internalized in DCs by conventional
phagocytosis, and cytochalasin D inhibited presentation of
bacteria-associated Ag, but not of soluble Ag, suggesting that
phagocytosis was required for proper delivery of recombinant Ag.
Bacteria were also very potent inducers of DC maturation, although they
enhanced the capacity of DCs to activate specific CD4+ T
cells at concentrations that did not stimulate DC maturation. In
particular, S. gordonii dose-dependently up-regulated
expression of membrane molecules (MHC I and II, CD80, CD86, CD54, CD40,
CD83) and reduced both phagocytic and endocytic activities.
Furthermore, bacteria promoted in a dose-dependent manner DC release of
cytokines (IL-6, TNF-
, IL-1ß, IL-12, TGF-ß, and IL-10) and of
the chemokines IL-8, RANTES, IFN--inducible protein-10, and monokine
induced by IFN-. Thus, recombinant Gram-positive bacteria appear a
powerful tool for vaccine design due to their extremely high capacity
to deliver Ags into DCs, as well as induce DC maturation and secretion
of T cell chemoattractans.
This article has been cited by other articles:
|
|
 |
|
  A. Ciabattini, E. Pettini, P. Andersen, G. Pozzi, and D. Medaglini Primary Activation of Antigen-Specific Naive CD4+ and CD8+ T Cells following Intranasal Vaccination with Recombinant Bacteria Infect. Immun., December 1, 2008; 76(12): 5817 - 5825. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Mariani, S. Gilles, T. Jakob, M. Thiel, M. J. Mueller, J. Ring, H. Behrendt, and C. Traidl-Hoffmann Immunomodulatory Mediators from Pollen Enhance the Migratory Capacity of Dendritic Cells and License Them for Th2 Attraction J. Immunol., June 15, 2007; 178(12): 7623 - 7631. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. G. Chan, M. Mayer, E. M. Davis, S. A. Halperin, T.-J. Lin, and S. F. Lee Role of D-Alanylation of Streptococcus gordonii Lipoteichoic Acid in Innate and Adaptive Immunity Infect. Immun., June 1, 2007; 75(6): 3033 - 3042. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ciabattini, A. M. Cuppone, R. Pulimeno, F. Iannelli, G. Pozzi, and D. Medaglini Stimulation of Human Monocytes with the Gram-Positive Vaccine Vector Streptococcus gordonii. Clin. Vaccine Immunol., September 1, 2006; 13(9): 1037 - 1043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Medaglini, A. Ciabattini, A. M. Cuppone, C. Costa, S. Ricci, M. Costalonga, and G. Pozzi In Vivo Activation of Naive CD4+ T Cells in Nasal Mucosa-Associated Lymphoid Tissue following Intranasal Immunization with Recombinant Streptococcus gordonii. Infect. Immun., May 1, 2006; 74(5): 2760 - 2766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-L. Hahn, H. A. Schenkein, and J. G. Tew Endocarditis-Associated Oral Streptococci Promote Rapid Differentiation of Monocytes into Mature Dendritic Cells Infect. Immun., August 1, 2005; 73(8): 5015 - 5021. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Grangette, H. Muller-Alouf, P. Hols, D. Goudercourt, J. Delcour, M. Turneer, and A. Mercenier Enhanced Mucosal Delivery of Antigen with Cell Wall Mutants of Lactic Acid Bacteria Infect. Immun., May 1, 2004; 72(5): 2731 - 2737. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Robinson, L. M. Chamberlain, M. C. Lopez, C. M. Rush, H. Marcotte, R. W. F. Le Page, and J. M. Wells Mucosal and Cellular Immune Responses Elicited by Recombinant Lactococcus lactis Strains Expressing Tetanus Toxin Fragment C Infect. Immun., May 1, 2004; 72(5): 2753 - 2761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Shimaoka, T. Nakayama, N. Kume, S. Takahashi, J. Yamaguchi, M. Minami, K. Hayashida, T. Kita, J. Ohsumi, O. Yoshie, et al. Cutting Edge: SR-PSOX/CXC Chemokine Ligand 16 Mediates Bacterial Phagocytosis by APCs Through its Chemokine Domain J. Immunol., August 15, 2003; 171(4): 1647 - 1651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nagl, L. Kacani, B. Mullauer, E.-M. Lemberger, H. Stoiber, G. M. Sprinzl, H. Schennach, and M. P. Dierich Phagocytosis and Killing of Bacteria by Professional Phagocytes and Dendritic Cells Clin. Vaccine Immunol., November 1, 2002; 9(6): 1165 - 1168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gorgun, K. B. Miller, and F. M. Foss Immunologic mechanisms of extracorporeal photochemotherapy in chronic graft-versus-host disease Blood, July 18, 2002; 100(3): 941 - 947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Unkmeir, U. Kammerer, A. Stade, C. Hubner, S. Haller, A. Kolb-Maurer, M. Frosch, and G. Dietrich Lipooligosaccharide and Polysaccharide Capsule: Virulence Factors of Neisseria meningitidis That Determine Meningococcal Interaction with Human Dendritic Cells Infect. Immun., May 1, 2002; 70(5): 2454 - 2462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Corinti, L. Chiarantini, S. Dominici, M. E. Laguardia, M. Magnani, and G. Girolomoni Erythrocytes deliver Tat to interferon-{gamma}-treated human dendritic cells for efficient initiation of specific type 1 immune responses in vitro J. Leukoc. Biol., April 1, 2002; 71(4): 652 - 658. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. C. Heystek, C. Moulon, A. M. Woltman, P. Garonne, and C. van Kooten Human Immature Dendritic Cells Efficiently Bind and Take up Secretory IgA Without the Induction of Maturation J. Immunol., January 1, 2002; 168(1): 102 - 107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. W. Cutler, R. Jotwani, and B. Pulendran Dendritic Cells: Immune Saviors or Achilles' Heel? Infect. Immun., August 1, 2001; 69(8): 4703 - 4708. [Full Text] [PDF]
|
|
|
|
|
|
A. Kolb-Maurer, S. Pilgrim, E. Kampgen, A. D. McLellan, E.-B. Brocker, W. Goebel, and I. Gentschev Antibodies against Listerial Protein 60 Act as an Opsonin for Phagocytosis of Listeria monocytogenes by Human Dendritic Cells Infect. Immun., May 1, 2001; 69(5): 3100 - 3109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Corinti, C. Albanesi, A. la Sala, S. Pastore, and G. Girolomoni Regulatory Activity of Autocrine IL-10 on Dendritic Cell Functions J. Immunol., April 1, 2001; 166(7): 4312 - 4318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Hertz, S. M. Kiertscher, P. J. Godowski, D. A. Bouis, M. V. Norgard, M. D. Roth, and R. L. Modlin Microbial Lipopeptides Stimulate Dendritic Cell Maturation Via Toll-Like Receptor 2 J. Immunol., February 15, 2001; 166(4): 2444 - 2450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Svensson, C. Johansson, and M. J. Wick Salmonella enterica Serovar Typhimurium-Induced Maturation of Bone Marrow-Derived Dendritic Cells Infect. Immun., November 1, 2000; 68(11): 6311 - 6320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. la Sala, S. Corinti, M. Federici, H. U. Saragovi, and G. Girolomoni Ligand activation of nerve growth factor receptor TrkA protects monocytes from apoptosis J. Leukoc. Biol., July 1, 2000; 68(1): 104 - 110. [Abstract] [Full Text]
|
|
|
|
|
|
A. Kolb-Maurer, I. Gentschev, H.-W. Fries, F. Fiedler, E.-B. Brocker, E. Kampgen, and W. Goebel Listeria monocytogenes-Infected Human Dendritic Cells: Uptake and Host Cell Response Infect. Immun., June 1, 2000; 68(6): 3680 - 3688. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Corinti, D. Medaglini, C. Prezzi, A. Cavani, G. Pozzi, and G. Girolomoni Human Dendritic Cells Are Superior to B Cells at Presenting a Major Histocompatibility Complex Class II-Restricted Heterologous Antigen Expressed on Recombinant Streptococcus gordonii Infect. Immun., April 1, 2000; 68(4): 1879 - 1883. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
