| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan;
National Institute of Health Sciences, Tokyo, Japan; and
Intestinal Disease Research Program, McMaster University, Hamilton, Canada
Communication between nerves and mast cells is a prototypic demonstration of neuroimmune interaction. However, whether mast cell activation occurs as a direct response to neuronal activation or requires an intermediary cell is unclear. Addressing this issue, we used an in vitro coculture approach comprising cultured murine superior cervical ganglia and rat leukemia basophilic cells (RBLs; possesses properties of mucosal-type mast cells). Following loading with the calcium fluorophore, Fluo-3, neurite-RBL units (separated by <50 nm) were examined by confocal laser scanning microscopy. Addition of bradykinin, or scorpion venom, dose-dependently elicited neurite activation (i.e., Ca2+ mobilization) and, after a lag period, RBL Ca2+ mobilization. Neither bradykinin nor scorpion venom had any direct effect on the RBLs in the absence of neurites. Addition of a neutralizing substance P Ab or a neurokinin (NK)-1 receptor antagonist, but not an NK-2 receptor antagonist, dose-dependently prevented the RBL activation that resulted as a consequence of neural activation by either bradykinin or scorpion venom. These data illustrate that nerve-mast cell cross-talk can occur in the absence of an intermediary transducing cell and that the neuropeptide substance P, operating via NK-1 receptors, is an important mediator of this communication. Our findings have implications for the neuroimmune signaling cascades that are likely to occur during airways inflammation, intestinal hypersensitivity, and other conditions in which mast cells feature.
This article has been cited by other articles:
|
|
 |
|
  M. Tagen, A. Elorza, D. Kempuraj, W. Boucher, C. L. Kepley, O. S. Shirihai, and T. C. Theoharides Mitochondrial Uncoupling Protein 2 Inhibits Mast Cell Activation and Reduces Histamine Content J. Immunol., November 15, 2009; 183(10): 6313 - 6319. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Xu, F. Xu, and E. Barrett Metalloelastase in lungs and alveolar macrophages is modulated by extracellular substance P in mice Am J Physiol Lung Cell Mol Physiol, July 1, 2008; 295(1): L162 - L170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhang, C.-L. Cang, Y. Kawasaki, L.-L. Liang, Y.-Q. Zhang, R.-R. Ji, and Z.-Q. Zhao Neurokinin-1 Receptor Enhances TRPV1 Activity in Primary Sensory Neurons via PKC{varepsilon}: A Novel Pathway for Heat Hyperalgesia J. Neurosci., October 31, 2007; 27(44): 12067 - 12077. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nowicki, D. Ostalska-Nowicka, B. Kondraciuk, and B. Miskowiak The significance of substance P in physiological and malignant haematopoiesis J. Clin. Pathol., July 1, 2007; 60(7): 749 - 755. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wang, X. Qiu, A. Kulkarni, and M. Hauer-Jensen Calcitonin Gene-Related Peptide and Substance P Regulate the Intestinal Radiation Response. Clin. Cancer Res., July 1, 2006; 12(13): 4112 - 4118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Furuno, A. Ito, Y.-i. Koma, K. Watabe, H. Yokozaki, J. Bienenstock, M. Nakanishi, and Y. Kitamura The Spermatogenic Ig Superfamily/Synaptic Cell Adhesion Molecule Mast-Cell Adhesion Molecule Promotes Interaction with Nerves J. Immunol., June 1, 2005; 174(11): 6934 - 6942. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S M Collins and G Barbara East meets West: infection, nerves, and mast cells in the irritable bowel syndrome Gut, August 1, 2004; 53(8): 1068 - 1069. [Full Text] [PDF]
|
|
|
|
 |
|
 F. De Jonge, A. De Laet, L. Van Nassauw, J. K. Brown, H. R. P. Miller, P.-P. van Bogaert, J.-P. Timmermans, and A. B. A. Kroese In vitro activation of murine DRG neurons by CGRP-mediated mucosal mast cell degranulation Am J Physiol Gastrointest Liver Physiol, July 1, 2004; 287(1): G178 - G191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Mori, R. Suzuki, T. Furuno, D. M. McKay, M. Wada, R. Teshima, J. Bienenstock, and M. Nakanishi Nerve-mast cell (RBL) interaction: RBL membrane ruffling occurs at the contact site with an activated neurite Am J Physiol Cell Physiol, December 1, 2002; 283(6): C1738 - C1744. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Mace, M. Jaume, C. Blanpied, L. Stephan, J. D. Coudert, P. Druet, and G. Dietrich Anti-{micro}-opioid-receptor IgG antibodies are commonly present in serum from healthy blood donors: evidence for a role in apoptotic immune cell death Blood, October 16, 2002; 100(9): 3261 - 3268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Path, A. Braun, N. Meents, S. Kerzel, D. Quarcoo, U. Raap, G. W. Hoyle, W. A. Nockher, and H. Renz Augmentation of Allergic Early-Phase Reaction by Nerve Growth Factor Am. J. Respir. Crit. Care Med., September 15, 2002; 166(6): 818 - 826. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Dianzani, G. Lombardi, M. Collino, C. Ferrara, M. C. Cassone, and R. Fantozzi Priming effects of substance P on calcium changes evoked by interleukin-8 in human neutrophils J. Leukoc. Biol., June 1, 2001; 69(6): 1013 - 1018. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Meredith Human Vomeronasal Organ Function: A Critical Review of Best and Worst Cases Chem Senses, May 1, 2001; 26(4): 433 - 445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. T. Green, N. W. Bunnett, A. Kulkarni-Narla, M. Steinhoff, and D. R. Brown Intestinal Type 2 Proteinase-Activated Receptors: Expression in Opioid-Sensitive Secretomotor Neural Circuits That Mediate Epithelial Ion Transport J. Pharmacol. Exp. Ther., October 1, 2000; 295(1): 410 - 416. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. Saban, M. R. Saban, N.-B. Nguyen, B. Lu, C. Gerard, N. P. Gerard, and T. G. Hammond Neurokinin-1 (NK-1) Receptor Is Required in Antigen-Induced Cystitis Am. J. Pathol., March 1, 2000; 156(3): 775 - 780. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
