The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stavnezer, J.
Right arrow Articles by Kenter, A. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stavnezer, J.
Right arrow Articles by Kenter, A. L.
The Journal of Immunology, 1999, 163: 2028-2040.
Copyright © 1999 by The American Association of Immunologists

Switch Recombination in a Transfected Plasmid Occurs Preferentially in a B Cell Line That Undergoes Switch Recombination of Its Chromosomal Ig Heavy Chain Genes1

Janet Stavnezer2,*, Sean P. Bradley*, Norman Rousseau*, Todd Pearson*, Ananth Shanmugam{dagger}, Debra J. Waite*, Paul R. Rogers* and Amy L. Kenter{dagger}

* Department of Molecular Genetics and Microbiology and Program in Immunology and Virology, University of Massachusetts Medical School, Worcester MA 01655; and {dagger} Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago IL 60612

Ab class switching is induced upon B cell activation in vivo by immunization or infection or in vitro by treatment with mitogens, e.g. LPS, and results in the expression of different heavy chain constant region (CH) genes without a change in the Ab variable region. This DNA recombination event allows Abs to alter their biological activity while maintaining their antigenic specificity. Little is known about the molecular mechanism of switch recombination. To attempt to develop an assay for enzymes, DNA binding proteins, and DNA sequences that mediate switch recombination, we have constructed a plasmid DNA substrate that will undergo switch recombination upon stable transfection into the surface IgM+ B cell line (I.29µ), a cell line capable of undergoing switch recombination of its endogenous genes. We demonstrate that recombination occurs between the two switch regions of the plasmid, as assayed by PCRs across the integrated plasmid switch regions, followed by Southern blot hybridization. Nucleotide sequence analysis of the PCR products confirmed the occurrence of Sµ-S{alpha} recombination in the plasmid. Recombination of the plasmid in I.29µ cells does not require treatment with inducers of switch recombination, suggesting that recombinase activity is constitutive in I.29µ cells. Recombination does not require high levels of transcription across the switch regions of the plasmid. Fewer recombination events are detected in four different B and T cell lines that do not undergo switch recombination of their endogenous genes.




This article has been cited by other articles:


Home page
 JEMHome page
A. A. Zarrin, P. H. Goff, K. Senger, and F. W. Alt
S{gamma}3 switch sequences function in place of endogenous S{gamma}1 to mediate antibody class switching
J. Exp. Med., July 7, 2008; 205(7): 1567 - 1572.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. P. Bradley, D. A. Kaminski, A. H. F. M. Peters, T. Jenuwein, and J. Stavnezer
The Histone Methyltransferase Suv39h1 Increases Class Switch Recombination Specifically to IgA
J. Immunol., July 15, 2006; 177(2): 1179 - 1188.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
A. L. Kenter, R. Wuerffel, C. Dominguez, A. Shanmugam, and H. Zhang
Mapping of a Functional Recombination Motif that Defines Isotype Specificity for {micro}->{gamma}3 Switch Recombination Implicates NF-{kappa}B p50 as the Isotype-specific Switching Factor
J. Exp. Med., March 1, 2004; 199(5): 617 - 627.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Ma, H. H. Wortis, and A. L. Kenter
Two New Isotype-Specific Switching Activities Detected for Ig Class Switching
J. Immunol., March 15, 2002; 168(6): 2835 - 2846.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. E. Drouin, C. E. Schrader, J. Stavnezer, and U. Hansen
The Ubiquitously Expressed DNA-Binding Protein Late SV40 Factor Binds Ig Switch Regions and Represses Class Switching to IgA
J. Immunol., March 15, 2002; 168(6): 2847 - 2856.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
T. M. Luby, C. E. Schrader, J. Stavnezer, and E. Selsing
The {micro} Switch Region Tandem Repeats Are Important, but Not Required, for Antibody Class Switch Recombination
J. Exp. Med., January 15, 2001; 193(2): 159 - 168.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
A. Shanmugam, M.-J. Shi, L. Yauch, J. Stavnezer, and A. L. Kenter
Evidence for Class-Specific Factors in Immunoglobulin Isotype Switching
J. Exp. Med., April 17, 2000; 191(8): 1365 - 1380.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M.-j. Li and N. Maizels
Activation and Targeting of Immunoglobulin Switch Recombination by Activities Induced by EBV Infection
J. Immunol., December 15, 1999; 163(12): 6659 - 6664.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.