| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Institut National de la Santé et de la Recherche Médicale, Unit 429, Hôpital Necker-Enfants Malades, Paris, France;
National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom; and
Institute Pasteur, Paris, France
2 Current address: Babraham Institute, Babraham, Cambridge, U.K.
Syk and ZAP-70 subserve nonredundant functions in B and T
lymphopoiesis. In the absence of Syk, B cell development is blocked,
while T cell development is arrested in the absence of ZAP-70. The
receptors and the signaling molecules required for differentiation of
NK cells are poorly characterized. Here we investigate the role of the
Syk protein tyrosine kinase in NK cell differentiation. Hemopoietic
chimeras were generated by reconstituting alymphoid (B-,
T-, NK-) recombinase-activating gene-2
x common cytokine receptor 
-chain double-mutant mice with
Syk-/- fetal liver cells. The
phenotypically mature Syk-/- NK cells that
developed in this context were fully competent in natural cytotoxicity
and in calibrating functional inhibitory receptors for MHC molecules.
Syk-deficient NK cells demonstrated reduced levels of Ab-dependent
cellular cytotoxicity. Nevertheless,
Syk-/- NK cells could signal
through NK1.1 and 2B4 activating receptors and expressed ZAP-70
protein. We conclude that the Syk protein tyrosine kinase is not
essential for murine NK cell development, and that compensatory
signaling pathways (including those mediated through ZAP-70) may
sustain most NK cell functions in the absence of
Syk.
This article has been cited by other articles:
|
|
 |
|
  M. F. R. Vondenhoff, G. E. Desanti, T. Cupedo, J. Y. Bertrand, A. Cumano, G. Kraal, R. E. Mebius, and R. Golub Separation of splenic red and white pulp occurs before birth in a LT{alpha}{beta}-independent manner J. Leukoc. Biol., July 1, 2008; 84(1): 152 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. E. Desanti, A. Cumano, and R. Golub Identification of CD4int progenitors in mouse fetal spleen, a source of resident lymphoid cells J. Leukoc. Biol., May 1, 2008; 83(5): 1145 - 1154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Y. Bertrand, G. E. Desanti, R. Lo-Man, C. Leclerc, A. Cumano, and R. Golub Fetal spleen stroma drives macrophage commitment Development, September 15, 2006; 133(18): 3619 - 3628. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. H. Mason, J. Willette-Brown, L. S. Taylor, and D. W. McVicar Regulation of Ly49D/DAP12 Signal Transduction by Src-Family Kinases and CD45. J. Immunol., June 1, 2006; 176(11): 6615 - 6623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Saborit-Villarroya, J. M. Del Valle, X. Romero, E. Esplugues, P. Lauzurica, P. Engel, and M. Martin The Adaptor Protein 3BP2 Binds Human CD244 and Links this Receptor to Vav Signaling, ERK Activation, and NK Cell Killing J. Immunol., October 1, 2005; 175(7): 4226 - 4235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. D. Huntington, Y. Xu, S. L. Nutt, and D. M. Tarlinton A requirement for CD45 distinguishes Ly49D-mediated cytokine and chemokine production from killing in primary natural killer cells J. Exp. Med., May 2, 2005; 201(9): 1421 - 1433. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Herre, A. S. J. Marshall, E. Caron, A. D. Edwards, D. L. Williams, E. Schweighoffer, V. Tybulewicz, C. R. e Sousa, S. Gordon, and G. D. Brown Dectin-1 uses novel mechanisms for yeast phagocytosis in macrophages Blood, December 15, 2004; 104(13): 4038 - 4045. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. D. Perez, D. Mitchell, G. C. Jager, and G. P. Nolan LFA-1 signaling through p44/42 is coupled to perforin degranulation in CD56+CD8+ natural killer cells Blood, August 15, 2004; 104(4): 1083 - 1093. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sedlik, D. Orbach, P. Veron, E. Schweighoffer, F. Colucci, R. Gamberale, A. Ioan-Facsinay, S. Verbeek, P. Ricciardi-Castagnoli, C. Bonnerot, et al. A Critical Role for Syk Protein Tyrosine Kinase in Fc Receptor-Mediated Antigen Presentation and Induction of Dendritic Cell Maturation J. Immunol., January 15, 2003; 170(2): 846 - 852. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Jiang, B. Zhong, C. Ritchey, D. L. Gilvary, E. Hong-Geller, S. Wei, and J. Y. Djeu Regulation of Akt-dependent cell survival by Syk and Rac Blood, January 1, 2003; 101(1): 236 - 244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. W. McVicar, R. Winkler-Pickett, L. S. Taylor, A. Makrigiannis, M. Bennett, S. K. Anderson, and J. R. Ortaldo Aberrant DAP12 Signaling in the 129 Strain of Mice: Implications for the Analysis of Gene-Targeted Mice J. Immunol., August 15, 2002; 169(4): 1721 - 1728. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Muller, L. Hunziker, S. Enzler, M. Buhler-Jungo, J. P. Di Santo, R. M. Zinkernagel, and C. Mueller Role of an Intact Splenic Microarchitecture in Early Lymphocytic Choriomeningitis Virus Production J. Virol., March 1, 2002; 76(5): 2375 - 2383. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Y. Djeu, K. Jiang, and S. Wei A View to a Kill: Signals Triggering Cytotoxicity Clin. Cancer Res., March 1, 2002; 8(3): 636 - 640. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Colucci, E. Rosmaraki, S. Bregenholt, S. I. Samson, V. Di Bartolo, M. Turner, L. Vanes, V. Tybulewicz, and J. P. Di Santo Functional Dichotomy in Natural Killer Cell Signaling: Vav1-Dependent and -Independent Mechanisms J. Exp. Med., June 18, 2001; 193(12): 1413 - 1424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Colucci, S. I. Samson, R. P. DeKoter, O. Lantz, H. Singh, and J. P. Di Santo Differential requirement for the transcription factor PU.1 in the generation of natural killer cells versus B and T cells Blood, May 1, 2001; 97(9): 2625 - 2632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Colucci, D. Guy-Grand, A. Wilson, M. Turner, E. Schweighoffer, V. L. J. Tybulewicz, and J. P. Di Santo A New Look at Syk in {alpha}{beta} and {gamma}{delta} T Cell Development Using Chimeric Mice with a Low Competitive Hematopoietic Environment J. Immunol., May 15, 2000; 164(10): 5140 - 5145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. L. Lanier Turning on Natural Killer Cells J. Exp. Med., April 17, 2000; 191(8): 1259 - 1262. [Full Text] [PDF]
|
|
|
|
|
|
F. Colucci and J. P. Di Santo The receptor tyrosine kinase c-kit provides a critical signal for survival, expansion, and maturation of mouse natural killer cells Blood, February 1, 2000; 95(3): 984 - 991. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
