| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Internal Medicine, Division of Pulmonary and Critical Care, and The Heart and Lung Institute, Ohio State University, Columbus, OH 43210
Apoptosis is an important mechanism for regulating the numbers of monocytes and macrophages. Caspases (cysteine-aspartate-specific proteases) are key molecules in apoptosis and require proteolytic removal of prodomains for activity. Caspase-1 and caspase-3 have both been connected to apoptosis in other model systems. The present study attempted to delineate what role these caspases play in spontaneous monocyte apoptosis. In serum-free conditions, monocytes showed a commitment to apoptosis as early as 4 h in culture, as evidenced by caspase-3-like activity. Apoptosis, as defined by oligonucleosomal DNA fragmentation, was prevented by a generalized caspase inhibitor, z-VAD-FMK, and the more specific caspase inhibitor, z-DEVD-FMK. The caspase activity was specifically attributable to caspase-3 by the identification of cleavage of procaspase-3 to active forms by immunoblots and by cleavage of the fluorogenic substrate DEVD-AFC. In contrast, a caspase-1 family inhibitor, YVAD-CMK, did not protect monocytes from apoptosis, and the fluorogenic substrate YVAD-AFC failed to show an increase in activity in apoptotic monocytes. When cultured with LPS (1 µg/ml), monocyte apoptosis was prevented, as was the activation of caspase-3. Unexpectedly, LPS did not change baseline caspase-1 activity. These findings link spontaneous monocyte apoptosis to the proteolytic activation of caspase-3.
This article has been cited by other articles:
|
|
 |
|
  C. Nicholas, S. Batra, M. A. Vargo, O. H. Voss, M. A. Gavrilin, M. D. Wewers, D. C. Guttridge, E. Grotewold, and A. I. Doseff Apigenin Blocks Lipopolysaccharide-Induced Lethality In Vivo and Proinflammatory Cytokines Expression by Inactivating NF-{kappa}B through the Suppression of p65 Phosphorylation J. Immunol., November 15, 2007; 179(10): 7121 - 7127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. H. Voss, S. Batra, S. J. Kolattukudy, M. E. Gonzalez-Mejia, J. B. Smith, and A. I. Doseff Binding of Caspase-3 Prodomain to Heat Shock Protein 27 Regulates Monocyte Apoptosis by Inhibiting Caspase-3 Proteolytic Activation J. Biol. Chem., August 24, 2007; 282(34): 25088 - 25099. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wang, M. M. Zeigler, G. K. Lam, M. G. Hunter, T. D. Eubank, V. V. Khramtsov, S. Tridandapani, C. K. Sen, and C. B. Marsh The Role of the NADPH Oxidase Complex, p38 MAPK, and Akt in Regulating Human Monocyte/Macrophage Survival Am. J. Respir. Cell Mol. Biol., January 1, 2007; 36(1): 68 - 77. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Sarkar, M. W. Hall, M. Exline, J. Hart, N. Knatz, N. T. Gatson, and M. D. Wewers Caspase-1 Regulates Escherichia coli Sepsis and Splenic B Cell Apoptosis Independently of Interleukin-1beta and Interleukin-18 Am. J. Respir. Crit. Care Med., November 1, 2006; 174(9): 1003 - 1010. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Oksjoki, P. T. Kovanen, K. A. Lindstedt, B. Jansson, and M. O. Pentikainen OxLDL-IgG Immune Complexes Induce Survival of Human Monocytes Arterioscler. Thromb. Vasc. Biol., March 1, 2006; 26(3): 576 - 583. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. H. Voss, S. Kim, M. D. Wewers, and A. I. Doseff Regulation of Monocyte Apoptosis by the Protein Kinase C{delta}-dependent Phosphorylation of Caspase-3 J. Biol. Chem., April 29, 2005; 280(17): 17371 - 17379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C. Denlinger, G. Angelini, K. Schell, D. N. Green, A. G. Guadarrama, U. Prabhu, D. B. Coursin, P. J. Bertics, and K. Hogan Detection of Human P2X7 Nucleotide Receptor Polymorphisms by a Novel Monocyte Pore Assay Predictive of Alterations in Lipopolysaccharide-Induced Cytokine Production J. Immunol., April 1, 2005; 174(7): 4424 - 4431. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Wu, R. D. Mosteller, and D. Broek Sphingosine Kinase Protects Lipopolysaccharide-Activated Macrophages from Apoptosis Mol. Cell. Biol., September 1, 2004; 24(17): 7359 - 7369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Wei, J. Guo, A. I. Doseff, D. F. Kusewitt, A. K. Man, R. G. Oshima, and M. C. Ostrowski Activated Ets2 Is Required for Persistent Inflammatory Responses in the Motheaten Viable Model J. Immunol., July 15, 2004; 173(2): 1374 - 1379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Wewers IL-1{beta}: An endosomal exit PNAS, July 13, 2004; 101(28): 10241 - 10242. [Full Text] [PDF]
|
|
|
|
|
|
A. Elssner, A. I. Doseff, M. Duncan, M. Kotur, and M. D. Wewers IL-16 Is Constitutively Present in Peripheral Blood Monocytes and Spontaneously Released During Apoptosis J. Immunol., June 15, 2004; 172(12): 7721 - 7725. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. I. Doseff, J. H. Baker Jr., T. A. Bourgeois, and M. D. Wewers Interleukin-4-Induced Apoptosis Entails Caspase Activation and Suppression of Extracellular Signal-Regulated Kinase Phosphorylation Am. J. Respir. Cell Mol. Biol., September 1, 2003; 29(3): 367 - 374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Park, A. R. Thomsen, C. W. Frevert, U. Pham, S. J. Skerrett, P. A. Kiener, and W. C. Liles Fas (CD95) Induces Proinflammatory Cytokine Responses by Human Monocytes and Monocyte-Derived Macrophages J. Immunol., June 15, 2003; 170(12): 6209 - 6216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Zeigler, A. I. Doseff, M. F. Galloway, J. M. Opalek, P. T. Nowicki, J. L. Zweier, C. K. Sen, and C. B. Marsh Presentation of Nitric Oxide Regulates Monocyte Survival through Effects on Caspase-9 and Caspase-3 Activation J. Biol. Chem., April 4, 2003; 278(15): 12894 - 12902. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Carracedo, R. Ramirez, S. Soriano, A. Martin-Malo, M. Rodriguez, and P. Aljama Caspase-3-dependent pathway mediates apoptosis of human mononuclear cells induced by cellulosic haemodialysis membranes Nephrol. Dial. Transplant., November 1, 2002; 17(11): 1971 - 1977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. J. Hathaway, G. E. Griffin, P. J. Sansonetti, and J. D. Edgeworth Human Monocytes Kill Shigella flexneri but Then Die by Apoptosis Associated with Suppression of Proinflammatory Cytokine Production Infect. Immun., July 1, 2002; 70(7): 3833 - 3842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Lang, F. Dohle, M. Terstesse, P. Bangen, C. August, H.-G. Pauels, and S. Heidenreich Down-Regulation of Monocyte Apoptosis by Phagocytosis of Platelets: Involvement of a Caspase-9, Caspase-3, and Heat Shock Protein 70-Dependent Pathway J. Immunol., June 15, 2002; 168(12): 6152 - 6158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-S. Bae, Y. Kim, J. C. Park, P.-G. Suh, and S. H. Ryu The synthetic chemoattractant peptide, Trp-Lys-Tyr-Met-Val-D-Met, enhances monocyte survival via PKC-dependent Akt activation J. Leukoc. Biol., February 1, 2002; 71(2): 329 - 338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Goyal, Y. Wang, M. M. Graham, A. I. Doseff, N. Y. Bhatt, and C. B. Marsh Monocyte Survival Factors Induce Akt Activation and Suppress Caspase-3 Am. J. Respir. Cell Mol. Biol., February 1, 2002; 26(2): 224 - 230. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. O. Kim, K. Ono, and J. Han Apoptosis by pan-caspase inhibitors in lipopolysaccharide-activated macrophages Am J Physiol Lung Cell Mol Physiol, November 1, 2001; 281(5): L1095 - L1105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ozaki and S. Hanazawa Porphyromonas gingivalis Fimbriae Inhibit Caspase-3-Mediated Apoptosis of Monocytic THP-1 Cells under Growth Factor Deprivation via Extracellular Signal-Regulated Kinase-Dependent Expression of p21 Cip/WAF1 Infect. Immun., August 1, 2001; 69(8): 4944 - 4950. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Sung Ouk Kim and Jiahuai Han Pan-caspase inhibitor zVAD enhances cell death in RAW246.7 macrophages Innate Immunity, August 1, 2001; 7(4): 292 - 296. [Abstract] [PDF]
|
|
|
|
|
|
M. Takahashi, M. Takahashi, F. Shinohara, H. Takada, and H. Rikiishi Effects of Superantigen and Lipopolysaccharide on Induction of CD80 through Apoptosis of Human Monocytes Infect. Immun., June 1, 2001; 69(6): 3652 - 3657. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kuhlmann, A. Bitsch, C. Stadelmann, H. Siebert, and W. Bruck Macrophages Are Eliminated from the Injured Peripheral Nerve via Local Apoptosis and Circulation to Regional Lymph Nodes and the Spleen J. Neurosci., May 15, 2001; 21(10): 3401 - 3408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-J. Ho, J.-J. Wang, M.-F. Shaio, C.-L. Kao, D.-M. Chang, S.-W. Han, and J.-H. Lai Infection of Human Dendritic Cells by Dengue Virus Causes Cell Maturation and Cytokine Production J. Immunol., February 1, 2001; 166(3): 1499 - 1506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Schmidt, N. Lugering, A. Lugering, H.-G. Pauels, K. Schulze-Osthoff, W. Domschke, and T. Kucharzik Role of the CD95/CD95 Ligand System in Glucocorticoid-Induced Monocyte Apoptosis J. Immunol., January 15, 2001; 166(2): 1344 - 1351. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
