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Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717
Nasal-associated lymphoid tissue (NALT), a mucosal inductive site
for the upper respiratory tract, is important for the development of
mucosal immunity locally and distally to intranasally introduced Ag. To
more fully understand the induction of nasal mucosal immunity, we
investigated the addressins that allow for lymphocyte trafficking to
this tissue. To investigate the addressins responsible for naive
lymphocyte binding, immunofluorescent and immunoperoxidase staining of
frozen NALT sections were performed using anti-mucosal addressin
cell adhesion molecule-1 (MAdCAM-1), anti-peripheral node addressin
(PNAd), and anti-VCAM-1 mAbs. All NALT high endothelial venules
(HEV) expressed PNAd, either associated with MAdCAM-1 or alone, whereas
NALT follicular dendritic cells expressed both MAdCAM-1 and VCAM-1.
These expression profiles were distinct from those of the gut mucosal
inductive site, Peyer’s patches (PP). The functionality of NALT HEV
was determined using a Stamper-Woodruff ex vivo assay. The
anti-L-selectin MEL-14 mAb blocked >90% of naive lymphocyte
binding to NALT HEV, whereas the anti-MAdCAM-1 mAb, which blocks
almost all naive lymphocyte binding to PP, minimally blocked binding to
NALT HEV. NALT lymphocytes exhibited a unique L-selectin expression
profile, differing from both PP and peripheral lymph nodes. Finally,
NALT HEV were found in increased amounts in the B cell zones, unlike PP
HEV. These results suggest that NALT is distinct from the intestinal
PP, that initial naive lymphocyte binding to NALT HEV involves
predominantly L-selectin and PNAd rather than
4
7-MAdCAM-1 interactions, and that
MAdCAM-1 and VCAM-1 expressed by NALT follicular dendritic cells may
play an important role in lymphocyte recruitment and
retention.
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