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*
Department of Medicine, School of Medicine, University of California at San Diego, La Jolla, CA 92093; and
Department of Medicine, School of Medicine, University of Pittsburgh, Pittsburgh, PA
We developed a murine IgG1 mAb, 5G9, following immunization
of a BALB/c mouse with Daudi cells. By immunoprecipitation, 5G9 reacted
with a 220-kDa Ag on Daudi cells, which reduced to four subunits (55,
65, 80, and 85 kDa). mAb 5G9 bound to 40–60% of peripheral blood B
cells, weakly reacted with monocytes and granulocytes, and did not bind
to erythrocytes, platelets, T cells, or NK cells. mAb 5G9 brightly
stained scattered cells in human tonsil sections, which appeared to be
dendritic cells (DC) by morphology. mAb 5G9 also stained scattered
cells in cytospin slides of monocyte-derived DC with long, thin, beaded
membrane processes, morphologically distinct from other
monocyte-derived DC. Positive selection of blood mononuclear cells with
mAb 5G9 and sheep anti-mouse IgG Dynabeads demonstrated an enriched
population of DC. By flow cytometry analysis, these cells were CD19,
CD20, CD22, CD40, CD44, CD83, CD86, IgD, and HLA-Dr positive and either
- or 
-L chain positive. They did not express CD3, CD4, CD5, CD10,
CD11b, CD13, CD25, CD56, CD14, CD33, or CD64. Isolated 5G9+
cells were potent APCs in allogeneic MLR, compared with
5G9- PBMC, 5G9- B cells, monocytes, and
monocytes cultured in IL-4 and GM-CSF for 24 h. mAb 5G9 defines a
novel peripheral blood cell with B cell phenotype and DC morphology and
function: DC-like B cells. The significance of this cell in immune
responses requires further study.
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