| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Division of Tumor Immunology, Dana-Farber Cancer Institute, and Department of Medicine, Harvard Medical School, Boston, MA 02115; and
Department of Clinical Immunology and AIDS Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan
ß1 integrins provide a costimulus for TCR/CD3-driven
T cell activation and IL-2 production in human peripheral T cells.
However, this ß1 integrin-mediated costimulation is
impaired in a human T lymphoblastic line, Jurkat. We studied the
molecular basis of this impaired costimulation and found that Cas-L, a
105-kDa docking protein, is marginally expressed in Jurkat T cells,
whereas Cas-L is well expressed in peripheral T cells. Cas-L is a
binding protein and a substrate for focal adhesion kinase and is
tyrosine phosphorylated by ß1 integrin stimulation. We
here show that the transfection of wild-type Cas-L in Jurkat T cells
restores ß1 integrin-mediated costimulation. However,
Cas-L transfection had no effect on CD28-mediated costimulation,
indicating that Cas-L is specifically involved in the ß1
integrin-mediated signaling pathway. Furthermore, transfection of the
Cas-L
SH3 mutant failed to restore ß1 integrin-mediated
costimulation in Jurkat cells. Cas-LSH3 mutant lacks the binding
site for focal adhesion kinase and is not tyrosine phosphorylated after
ß1 integrin stimulation. These findings strongly suggest
that the tyrosine phosphorylation of Cas-L plays a key role in the
signal transduction in the ß1 integrin-mediated T cell
costimulation.
This article has been cited by other articles:
|
|
 |
|
  T. Sasaki, S. Iwata, H. J. Okano, Y. Urasaki, J. Hamada, H. Tanaka, N. H. Dang, H. Okano, and C. Morimoto Nedd9 Protein, a Cas-L Homologue, Is Upregulated After Transient Global Ischemia in Rats: Possible Involvement of Nedd9 in the Differentiation of Neurons After Ischemia Stroke, November 1, 2005; 36(11): 2457 - 2462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Feng, S. Guedes, and T. Wang Atrophin-1-interacting Protein 4/Human Itch Is a Ubiquitin E3 Ligase for Human Enhancer of Filamentation 1 in Transforming Growth Factor-{beta} Signaling Pathways J. Biol. Chem., July 9, 2004; 279(28): 29681 - 29690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sakakibara, S. Hattori, S. Nakamura, and T. Katagiri A Novel Hematopoietic Adaptor Protein, Chat-H, Positively Regulates T Cell Receptor-mediated Interleukin-2 Production by Jurkat Cells J. Biol. Chem., February 14, 2003; 278(8): 6012 - 6017. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Li, M. J. Calzada, J. M. Sipes, J. A. Cashel, H. C. Krutzsch, D. S. Annis, D. F. Mosher, and D. D. Roberts Interactions of thrombospondins with {alpha}4{beta}1 integrin and CD47 differentially modulate T cell behavior J. Cell Biol., April 29, 2002; 157(3): 509 - 519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Suzuki, T. Nakamoto, S. Ogawa, S. Seo, T. Matsumura, K. Tachibana, C. Morimoto, and H. Hirai MICAL, a Novel CasL Interacting Molecule, Associates with Vimentin J. Biol. Chem., April 19, 2002; 277(17): 14933 - 14941. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Li, M. J. Calzada, J. M. Sipes, J. A. Cashel, H. C. Krutzsch, D. S. Annis, D. F. Mosher, and D. D. Roberts Interactions of thrombospondins with {alpha}4{beta}1 integrin and CD47 differentially modulate T cell behavior J. Cell Biol., April 29, 2002; 157(3): 509 - 519. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
