HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Iwasaki, H. Articles by Niho, Y. Search for Related Content PubMed PubMed Citation Articles by Iwasaki, H. Articles by Niho, Y.

Production of Soluble Granulocyte Colony-Stimulating Factor Receptors from Myelomonocytic Cells¹

Hiromi Iwasaki^2,*, Kazuya Shimoda^*, Seiichi Okamura^*, Teruhisa Otsuka^*, Koji Nagafuji^*, Naoki Harada^*, Yuju Ohno^*, Toshihiro Miyamoto^*, Koichi Akashi^3,*, Mine Harada and Yoshiyuki Niho^*

^* First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan; and Second Department of Internal Medicine, Okayama University School of Medicine, Okayama, Japan

It has been speculated that a soluble form of G-CSFR might be physiologically present in humans, since G-CSFR mRNA that lacks a transmembrane domain has been identified from a human myelomonocytic cell line. Here, we demonstrate human soluble G-CSFR (sG-CSFR) of two different molecular sizes (80 and 85 kDa) on an immunoblot analysis using Abs generated against the amino-terminal, extracellular domain of the full-length G-CSFR. Both isoforms of sG-CSFR were able to bind recombinant human G-CSF (rhG-CSF). RT-PCR analysis with primers targeted outside of the transmenbrane region revealed that membrane-anchored G-CSFR is expressed at all maturation stages of purified myeloid cells, including CD34⁺CD13⁺ cells (blasts), CD11b^-CD15⁺ cells (promyelocytes or myelocytes), CD11b⁺CD15⁺ cells (metamyelocytes and mature neutrophils), and CD14⁺ cells (monocytes). On the other hand, sG-CSFR mRNA was detectable in CD11b^-CD15⁺, CD11b⁺CD15⁺, and CD14⁺ cells, but not in the CD34⁺CD13⁺ blast population. The serum concentration of both isoforms of sG-CSFR appeared to be correlated with the numbers of neutrophils/monocytes before and after rhG-CSF treatment in normal individuals. Thus, two isoforms of sG-CSFR are physiologically secreted from relatively mature myeloid cells and might play an important role in myelopoiesis through their binding to serum G-CSF.

This article has been cited by other articles:

				M. Fukuda, M. Oka, Y. Ishida, H. Kinoshita, K. Terashi, M. Fukuda, S. Kawabata, A. Kinoshita, H. Soda, and S. Kohno Effects of Renal Function on Pharmacokinetics of Recombinant Human Granulocyte Colony-Stimulating Factor in Lung Cancer Patients Antimicrob. Agents Chemother., July 1, 2001; 45(7): 1947 - 1951. [Abstract] [Full Text] [PDF]