The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kammula, U. S.
Right arrow Articles by Marincola, F. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kammula, U. S.
Right arrow Articles by Marincola, F. M.
The Journal of Immunology, 1999, 163: 6867-6875.
Copyright © 1999 by The American Association of Immunologists

Functional Analysis of Antigen-Specific T Lymphocytes by Serial Measurement of Gene Expression in Peripheral Blood Mononuclear Cells and Tumor Specimens

Udai S. Kammula*, Kang-Hun Lee{dagger}, Adam I Riker*, Ena Wang*, Galen A. Ohnmacht*, Steven A. Rosenberg* and Francesco M. Marincola1,{dagger}

* Surgery Branch and {dagger} Department of Transfusion Medicine, Clinical Center, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892

The cloning of cancer Ags recognized by T cells has provided potentially new tools to enhance immunity against metastatic cancer. The biological monitoring of effective immunization has, however, remained a dilemma. We describe here a sensitive molecular quantitation methodology that allows analysis of in vivo immune response to vaccination. Metastatic melanoma patients were immunized with a synthetically modified peptide epitope (209-2M) from the melanoma self-Ag gp100. Using serial gene expression analysis, we report functional evidence of vaccine-induced CTL reactivity in fresh cells obtained directly from the peripheral blood of postimmunized patients. Further, we demonstrate in vivo localization of vaccine-induced immune response within the tumor microenvironment. The results of these molecular assays provide direct evidence that peptide immunization in humans can result in tumor-specific CTL that localize to metastatic sites.




This article has been cited by other articles:


Home page
 Clin. Cancer Res.Home page
F. O. Smith, S. G. Downey, J. A. Klapper, J. C. Yang, R. M. Sherry, R. E. Royal, U. S. Kammula, M. S. Hughes, N. P. Restifo, C. L. Levy, et al.
Treatment of Metastatic Melanoma Using Interleukin-2 Alone or in Conjunction with Vaccines
Clin. Cancer Res., September 1, 2008; 14(17): 5610 - 5618.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C. J. Wheeler, K. L. Black, G. Liu, M. Mazer, X.-x. Zhang, S. Pepkowitz, D. Goldfinger, H. Ng, D. Irvin, and J. S. Yu
Vaccination Elicits Correlated Immune and Clinical Responses in Glioblastoma Multiforme Patients
Cancer Res., July 15, 2008; 68(14): 5955 - 5964.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
V. Revaz, A. Debonneville, M. Bobst, and D. Nardelli-Haefliger
Monitoring of Vaccine-Specific Gamma Interferon Induction in Genital Mucosa of Mice by Real-Time Reverse Transcription-PCR
Clin. Vaccine Immunol., May 1, 2008; 15(5): 757 - 764.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
E. Fonsatti, H. J.M. Nicolay, L. Sigalotti, L. Calabro, L. Pezzani, F. Colizzi, M. Altomonte, M. Guidoboni, F. M. Marincola, and M. Maio
Functional Up-regulation of Human Leukocyte Antigen Class I Antigens Expression by 5-aza-2'-deoxycytidine in Cutaneous Melanoma: Immunotherapeutic Implications
Clin. Cancer Res., June 1, 2007; 13(11): 3333 - 3338.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
Z. Liu, M. Jiang, J. Zhao, and H. Ju
Circulating Tumor Cells in Perioperative Esophageal Cancer Patients: Quantitative Assay System and Potential Clinical Utility
Clin. Cancer Res., May 15, 2007; 13(10): 2992 - 2997.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. Grube, S. Moritz, E. C. Obermann, K. Rezvani, A. Mackensen, R. Andreesen, and E. Holler
CD8+ T cells Reactive to Survivin Antigen in Patients with Multiple Myeloma
Clin. Cancer Res., February 1, 2007; 13(3): 1053 - 1060.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. E. Nair, M. O. Kilinc, S. A. Jones, and N. K. Egilmez
Chronic Immune Therapy Induces a Progressive Increase in Intratumoral T Suppressor Activity and a Concurrent Loss of Tumor-Specific CD8+ T Effectors in her-2/neu Transgenic Mice Bearing Advanced Spontaneous Tumors.
J. Immunol., June 15, 2006; 176(12): 7325 - 7334.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
K. Rezvani, J. M. Brenchley, D. A. Price, Y. Kilical, E. Gostick, A. K. Sewell, J. Li, S. Mielke, D. C. Douek, and A. J. Barrett
T-Cell Responses Directed against Multiple HLA-A*0201-Restricted Epitopes Derived from Wilms' Tumor 1 Protein in Patients with Leukemia and Healthy Donors: Identification, Quantification, and Characterization
Clin. Cancer Res., December 15, 2005; 11(24): 8799 - 8807.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
C. J. Wheeler, A. Das, G. Liu, J. S. Yu, and K. L. Black
Clinical Responsiveness of Glioblastoma Multiforme to Chemotherapy after Vaccination
Clin. Cancer Res., August 15, 2004; 10(16): 5316 - 5326.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. S. Yu, G. Liu, H. Ying, W. H. Yong, K. L. Black, and C. J. Wheeler
Vaccination with Tumor Lysate-Pulsed Dendritic Cells Elicits Antigen-Specific, Cytotoxic T-Cells in Patients with Malignant Glioma
Cancer Res., July 15, 2004; 64(14): 4973 - 4979.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. Grube, K. Rezvani, A. Wiestner, H. Fujiwara, G. Sconocchia, J. J. Melenhorst, N. Hensel, G. E. Marti, L. W. Kwak, W. Wilson, et al.
Autoreactive, Cytotoxic T Lymphocytes Specific for Peptides Derived from Normal B-Cell Differentiation Antigens in Healthy Individuals and Patients with B-Cell Malignancies
Clin. Cancer Res., February 1, 2004; 10(3): 1047 - 1056.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. J. Wheeler, K. L. Black, G. Liu, H. Ying, J. S. Yu, W. Zhang, and P. K. Lee
Thymic CD8+ T Cell Production Strongly Influences Tumor Antigen Recognition and Age-Dependent Glioma Mortality
J. Immunol., November 1, 2003; 171(9): 4927 - 4933.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K. Rezvani, M. Grube, J. M. Brenchley, G. Sconocchia, H. Fujiwara, D. A. Price, E. Gostick, K. Yamada, J. Melenhorst, R. Childs, et al.
Functional leukemia-associated antigen-specific memory CD8+ T cells exist in healthy individuals and in patients with chronic myelogenous leukemia before and after stem cell transplantation
Blood, October 15, 2003; 102(8): 2892 - 2900.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
C. Rentzsch, S. Kayser, S. Stumm, I. Watermann, S. Walter, S. Stevanovic, D. Wallwiener, and B. Guckel
Evaluation of Pre-existent Immunity in Patients with Primary Breast Cancer: Molecular and Cellular Assays to Quantify Antigen-Specific T Lymphocytes in Peripheral Blood Mononuclear Cells
Clin. Cancer Res., October 1, 2003; 9(12): 4376 - 4386.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. Q. Phan, J. C. Yang, R. M. Sherry, P. Hwu, S. L. Topalian, D. J. Schwartzentruber, N. P. Restifo, L. R. Haworth, C. A. Seipp, L. J. Freezer, et al.
Cancer regression and autoimmunity induced by cytotoxic T lymphocyte-associated antigen 4 blockade in patients with metastatic melanoma
PNAS, July 8, 2003; 100(14): 8372 - 8377.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
G. J. Ullenhag, J.-E. Frodin, S. Mosolits, S. Kiaii, M. Hassan, M. C. Bonnet, P. Moingeon, H. Mellstedt, and H. Rabbani
Immunization of Colorectal Carcinoma Patients with a Recombinant Canarypox Virus Expressing the Tumor Antigen Ep-CAM/KSA (ALVAC-KSA) and Granulocyte Macrophage Colony- stimulating Factor Induced a Tumor-specific Cellular Immune Response
Clin. Cancer Res., July 1, 2003; 9(7): 2447 - 2456.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
C. Palena, J. Schlom, and K.-Y. Tsang
Differential Gene Expression Profiles in a Human T-cell Line Stimulated with a Tumor-associated Self-peptide versus an Enhancer Agonist Peptide
Clin. Cancer Res., May 1, 2003; 9(5): 1616 - 1627.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. G. Carrabba, C. Castelli, M. J. Maeurer, P. Squarcina, A. Cova, L. Pilla, N. Renkvist, G. Parmiani, and L. Rivoltini
Suboptimal Activation of CD8+ T Cells by Melanoma-derived Altered Peptide Ligands: Role of Melan-A/MART-1 Optimized Analogues
Cancer Res., April 1, 2003; 63(7): 1560 - 1567.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
T. Weinschenk, C. Gouttefangeas, M. Schirle, F. Obermayr, S. Walter, O. Schoor, R. Kurek, W. Loeser, K.-H. Bichler, D. Wernet, et al.
Integrated Functional Genomics Approach for the Design of Patient-individual Antitumor Vaccines
Cancer Res., October 15, 2002; 62(20): 5818 - 5827.
[Abstract] [Full Text] [PDF]

Home page
 JNCI J Natl Cancer InstHome page
G. Parmiani, C. Castelli, P. Dalerba, R. Mortarini, L. Rivoltini, F. M. Marincola, and A. Anichini
Cancer Immunotherapy With Peptide-Based Vaccines: What Have We Achieved? Where Are We Going?
J Natl Cancer Inst, June 5, 2002; 94(11): 805 - 818.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
V. Monsurro, D. Nagorsen, E. Wang, M. Provenzano, M. E. Dudley, S. A. Rosenberg, and F. M. Marincola
Functional Heterogeneity of Vaccine-Induced CD8+ T Cells
J. Immunol., June 1, 2002; 168(11): 5933 - 5942.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
I. C. Le Poole, A. I. Riker, M. E. Quevedo, L. S. Stennett, E. Wang, F. M. Marincola, W. M. Kast, J. K. Robinson, and B. J. Nickoloff
Interferon-{gamma} Reduces Melanosomal Antigen Expression and Recognition of Melanoma Cells by Cytotoxic T Cells
Am. J. Pathol., February 1, 2002; 160(2): 521 - 528.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. T. Khong and S. A. Rosenberg
Pre-Existing Immunity to Tyrosinase-Related Protein (TRP)-2, a New TRP-2 Isoform, and the NY-ESO-1 Melanoma Antigen in a Patient with a Dramatic Response to Immunotherapy
J. Immunol., January 15, 2002; 168(2): 951 - 956.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Perez-Diez, P. J. Spiess, N. P. Restifo, P. Matzinger, and F. M. Marincola
Intensity of the Vaccine-Elicited Immune Response Determines Tumor Clearance
J. Immunol., January 1, 2002; 168(1): 338 - 347.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. A. Ohnmacht, E. Wang, S. Mocellin, A. Abati, A. Filie, P. Fetsch, A. I. Riker, U. S. Kammula, S. A. Rosenberg, and F. M. Marincola
Short-Term Kinetics of Tumor Antigen Expression in Response to Vaccination
J. Immunol., August 1, 2001; 167(3): 1809 - 1820.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
K. Schumacher, W. Haensch, C. Röefzaad, and P. M. Schlag
Prognostic Significance of Activated CD8+ T Cell Infiltrations within Esophageal Carcinomas
Cancer Res., May 1, 2001; 61(10): 3932 - 3936.
[Abstract] [Full Text]

Home page
 Clin. Cancer Res.Home page
T. M. Clay, A. C. Hobeika, P. J. Mosca, H. K. Lyerly, and M. A. Morse
Assays for Monitoring Cellular Immune Responses to Active Immunotherapy of Cancer
Clin. Cancer Res., May 1, 2001; 7(5): 1127 - 1135.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
V. Monsurro, M.-B. Nielsen, A. Perez-Diez, M. E. Dudley, E. Wang, S. A. Rosenberg, and F. M. Marincola
Kinetics of TCR Use in Response to Repeated Epitope-Specific Immunization
J. Immunol., May 1, 2001; 166(9): 5817 - 5825.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
K. Shimizu, E. K. Thomas, M. Giedlin, and J. J. Mulé
Enhancement of Tumor Lysate- and Peptide-pulsed Dendritic Cell-based Vaccines by the Addition of Foreign Helper Protein
Cancer Res., March 1, 2001; 61(6): 2618 - 2624.
[Abstract] [Full Text]

Home page
 Ann. Thorac. Surg.Home page
T. S. Weiser, G. A. Ohnmacht, Z. S. Guo, M. R. Fischette, G. A. Chen, J. A. Hong, D. M. Nguyen, and D. S. Schrump
Induction of MAGE-3 expression in lung and esophageal cancer cells
Ann. Thorac. Surg., January 1, 2001; 71(1): 295 - 302.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. V. Dhodapkar, J. W. Young, P. B. Chapman, W. I. Cox, J. F. Fonteneau, S. Amigorena, A. N. Houghton, R. M. Steinman, and N. Bhardwaj
Paucity of Functional T-Cell Memory to Melanoma Antigens in Healthy Donors and Melanoma Patients
Clin. Cancer Res., December 1, 2000; 6(12): 4831 - 4838.
[Abstract] [Full Text]

Home page
 JNCI J Natl Cancer InstHome page
U. S. Kammula, F. M. Marincola, and S. A. Rosenberg
Real-Time Quantitative Polymerase Chain Reaction Assessment of Immune Reactivity in Melanoma Patients After Tumor Peptide Vaccination
J Natl Cancer Inst, August 16, 2000; 92(16): 1336 - 1344.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M.-B. Nielsen, V. Monsurro, S. A. Migueles, E. Wang, A. Perez-Diez, K.-H. Lee, U. Kammula, S. A. Rosenberg, and F. M. Marincola
Status of Activation of Circulating Vaccine-Elicited CD8+ T Cells
J. Immunol., August 15, 2000; 165(4): 2287 - 2296.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. C. Panelli, M. P. Bettinotti, K. Lally, G. A. Ohnmacht, Y. Li, P. Robbins, A. Riker, S. A. Rosenberg, and F. M. Marincola
A Tumor-Infiltrating Lymphocyte from a Melanoma Metastasis with Decreased Expression of Melanoma Differentiation Antigens Recognizes MAGE-12
J. Immunol., April 15, 2000; 164(8): 4382 - 4392.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.