The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by DeLeo, F. R.
Right arrow Articles by Nauseef, W. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by DeLeo, F. R.
Right arrow Articles by Nauseef, W. M.
The Journal of Immunology, 1999, 163: 6732-6740.
Copyright © 1999 by The American Association of Immunologists

NADPH Oxidase Activation and Assembly During Phagocytosis1

Frank R. DeLeo*,{dagger}, Lee-Ann H. Allen*,{dagger}, Michael Apicella{ddagger} and William M. Nauseef2,*,{dagger}

* Inflammation Program and Departments of {dagger} Medicine and {ddagger} Microbiology, Veterans Administration Medical Center and University of Iowa, Iowa City, IA 52246

Generation of superoxide (O2-) by the NADPH-dependent oxidase of polymorphonuclear leukocytes is an essential component of the innate immune response to invading microorganisms. To examine NADPH oxidase function during phagocytosis, we evaluated its activation and assembly following ingestion of serum-opsonized Neisseria meningitidis, serogroup B (NMB), and compared it with that elicited by serum-opsonized zymosan (OPZ). Opsonized N. meningitidis- and OPZ-dependent generation of reactive oxygen species by polymorphonuclear leukocytes peaked early and then terminated. Phosphorylation of p47phox coincided with peak generation of reactive oxygen species by either stimulus, consistent with a role for p47phox phosphorylation during NADPH oxidase activation, and correlated with phagosomal colocalization of flavocytochrome b558 (flavocytochrome b) and p47phox and p67phox (p47/67phox). Termination of respiratory burst activity did not reflect dephosphorylation of plasma membrane- and/or phagosome-associated p47phox; in contrast, the specific activity of phosphorylated p47phox at the phagosomal membrane increased. Most significantly, termination of oxidase activity paralleled the loss of p47/67phox from both NMB and OPZ phagosomes despite the continued presence of flavocytochrome b. These data suggest that 1) the onset of respiratory burst activity during phagocytosis is linked to the phosphorylation of p47phox and its translocation to the phagosome; and 2) termination of oxidase activity correlates with loss of p47/67phox from flavocytochrome b-enriched phagosomes and additional phosphorylation of membrane-associated p47phox.




This article has been cited by other articles:


Home page
 Infect. Immun.Home page
B. W. Buchan, R. L. McCaffrey, S. R. Lindemann, L.-A. H. Allen, and B. D. Jones
Identification of migR, a Regulatory Element of the Francisella tularensis Live Vaccine Strain iglABCD Virulence Operon Required for Normal Replication and Trafficking in Macrophages
Infect. Immun., June 1, 2009; 77(6): 2517 - 2529.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
G. S. Schulert, R. L. McCaffrey, B. W. Buchan, S. R. Lindemann, C. Hollenback, B. D. Jones, and L.-A. H. Allen
Francisella tularensis Genes Required for Inhibition of the Neutrophil Respiratory Burst and Intramacrophage Growth Identified by Random Transposon Mutagenesis of Strain LVS
Infect. Immun., April 1, 2009; 77(4): 1324 - 1336.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
X. J. Li, W. Tian, N. D. Stull, S. Grinstein, S. Atkinson, and M. C. Dinauer
A Fluorescently Tagged C-Terminal Fragment of p47phox Detects NADPH Oxidase Dynamics during Phagocytosis
Mol. Biol. Cell, March 1, 2009; 20(5): 1520 - 1532.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
W. Tian, X. J. Li, N. D. Stull, W. Ming, C.-I. Suh, S. A. Bissonnette, M. B. Yaffe, S. Grinstein, S. J. Atkinson, and M. C. Dinauer
Fc{gamma}R-stimulated activation of the NADPH oxidase: phosphoinositide-binding protein p40phox regulates NADPH oxidase activity after enzyme assembly on the phagosome
Blood, November 1, 2008; 112(9): 3867 - 3877.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
P. Sumby, S. Zhang, A. R. Whitney, F. Falugi, G. Grandi, E. A. Graviss, F. R. DeLeo, and J. M. Musser
A Chemokine-Degrading Extracellular Protease Made by Group A Streptococcus Alters Pathogenesis by Enhancing Evasion of the Innate Immune Response
Infect. Immun., March 1, 2008; 76(3): 978 - 985.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. A. Bissonnette, C. M. Glazier, M. Q. Stewart, G. E. Brown, C. D. Ellson, and M. B. Yaffe
Phosphatidylinositol 3-Phosphate-dependent and -independent Functions of p40phox in Activation of the Neutrophil NADPH Oxidase
J. Biol. Chem., January 25, 2008; 283(4): 2108 - 2119.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
T. L. Herrmann, R. S. Agrawal, S. F. Connolly, R. L. McCaffrey, J. Schlomann, and D. J. Kusner
MHC Class II levels and intracellular localization in human dendritic cells are regulated by calmodulin kinase II
J. Leukoc. Biol., September 1, 2007; 82(3): 686 - 699.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
J. S. Lee, W. M. Nauseef, A. Moeenrezakhanlou, L. M. Sly, S. Noubir, K. G. Leidal, J. M. Schlomann, G. Krystal, and N. E. Reiner
Monocyte p110{alpha} phosphatidylinositol 3-kinase regulates phagocytosis, the phagocyte oxidase, and cytokine production
J. Leukoc. Biol., June 1, 2007; 81(6): 1548 - 1561.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
C.-S. Yang, D.-S. Lee, C.-H. Song, S.-J. An, S. Li, J.-M. Kim, C. S. Kim, D. G. Yoo, B. H. Jeon, H.-Y. Yang, et al.
Roles of peroxiredoxin II in the regulation of proinflammatory responses to LPS and protection against endotoxin-induced lethal shock
J. Exp. Med., March 19, 2007; 204(3): 583 - 594.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. M. Lavigne, J. E. Albina, and J. S. Reichner
beta-Glucan Is a Fungal Determinant for Adhesion-Dependent Human Neutrophil Functions
J. Immunol., December 15, 2006; 177(12): 8667 - 8675.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
R. L. McCaffrey and L.-A. H. Allen
Francisella tularensis LVS evades killing by human neutrophils via inhibition of the respiratory burst and phagosome escape
J. Leukoc. Biol., December 1, 2006; 80(6): 1224 - 1230.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. S. Daniel, G. Dai, C. R. Singh, D. R. Lindsey, A. K. Smith, S. Dhandayuthapani, R. L. Hunter Jr, and C. Jagannath
The Reduced Bactericidal Function of Complement C5-Deficient Murine Macrophages Is Associated with Defects in the Synthesis and Delivery of Reactive Oxygen Radicals to Mycobacterial Phagosomes
J. Immunol., October 1, 2006; 177(7): 4688 - 4698.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
P. M.-C. Dang, C. Elbim, J.-C. Marie, M. Chiandotto, M.-A. Gougerot-Pocidalo, and J. El-Benna
Anti-inflammatory effect of interleukin-10 on human neutrophil respiratory burst involves inhibition of GM-CSF-induced p47PHOX phosphorylation through a decrease in ERK1/2 activity
FASEB J, July 1, 2006; 20(9): 1504 - 1506.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. G. Moreland, A. P. Davis, G. Bailey, W. M. Nauseef, and Fred. S. Lamb
Anion Channels, Including ClC-3, Are Required for Normal Neutrophil Oxidative Function, Phagocytosis, and Transendothelial Migration
J. Biol. Chem., May 5, 2006; 281(18): 12277 - 12288.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
C. Burlak, A. R. Whitney, D. J. Mead, T. Hackstadt, and F. R. DeLeo
Maturation of Human Neutrophil Phagosomes Includes Incorporation of Molecular Chaperones and Endoplasmic Reticulum Quality Control Machinery
Mol. Cell. Proteomics, April 1, 2006; 5(4): 620 - 634.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Balestrieri, V. W. Hsu, H. Gilbert, C. C. Leslie, W. K. Han, J. V. Bonventre, and J. P. Arm
Group V Secretory Phospholipase A2 Translocates to the Phagosome after Zymosan Stimulation of Mouse Peritoneal Macrophages and Regulates Phagocytosis
J. Biol. Chem., March 10, 2006; 281(10): 6691 - 6698.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
S.-K. Heo, S.-A Ju, S.-C. Lee, S.-M. Park, S.-Y. Choe, B. Kwon, B. S. Kwon, and B.-S. Kim
LIGHT enhances the bactericidal activity of human monocytes and neutrophils via HVEM
J. Leukoc. Biol., February 1, 2006; 79(2): 330 - 338.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
C. Kim and M. C. Dinauer
Impaired NADPH oxidase activity in Rac2-deficient murine neutrophils does not result from defective translocation of p47phox and p67phox and can be rescued by exogenous arachidonic acid
J. Leukoc. Biol., January 1, 2006; 79(1): 223 - 234.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
F. A. Khanday, T. Yamamori, I. Mattagajasingh, Z. Zhang, A. Bugayenko, A. Naqvi, L. Santhanam, N. Nabi, K. Kasuno, B. W. Day, et al.
Rac1 Leads to Phosphorylation-dependent Increase in Stability of the p66shc Adaptor Protein: Role in Rac1-induced Oxidative Stress
Mol. Biol. Cell, January 1, 2006; 17(1): 122 - 129.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
S. D. Kobayashi, J. M. Voyich, A. R. Whitney, and F. R. DeLeo
Spontaneous neutrophil apoptosis and regulation of cell survival by granulocyte macrophage-colony stimulating factor
J. Leukoc. Biol., December 1, 2005; 78(6): 1408 - 1418.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. M. Voyich, K. R. Braughton, D. E. Sturdevant, A. R. Whitney, B. Said-Salim, S. F. Porcella, R. D. Long, D. W. Dorward, D. J. Gardner, B. N. Kreiswirth, et al.
Insights into Mechanisms Used by Staphylococcus aureus to Avoid Destruction by Human Neutrophils
J. Immunol., September 15, 2005; 175(6): 3907 - 3919.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. L. Borjesson, S. D. Kobayashi, A. R. Whitney, J. M. Voyich, C. M. Argue, and F. R. DeLeo
Insights into Pathogen Immune Evasion Mechanisms: Anaplasma phagocytophilum Fails to Induce an Apoptosis Differentiation Program in Human Neutrophils
J. Immunol., May 15, 2005; 174(10): 6364 - 6372.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L.-A. H. Allen, B. R. Beecher, J. T. Lynch, O. V. Rohner, and L. M. Wittine
Helicobacter pylori Disrupts NADPH Oxidase Targeting in Human Neutrophils to Induce Extracellular Superoxide Release
J. Immunol., March 15, 2005; 174(6): 3658 - 3667.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
C. Elbim, C. Guichard, P. M. C. Dang, M. Fay, E. Pedruzzi, H. Demur, C. Pouzet, J. El Benna, and M.-A. Gougerot-Pocidalo
Interleukin-18 Primes the Oxidative Burst of Neutrophils in Response to Formyl-Peptides: Role of Cytochrome b558 Translocation and N-Formyl Peptide Receptor Endocytosis
Clin. Vaccine Immunol., March 1, 2005; 12(3): 436 - 446.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Ueyama, M. R. Lennartz, Y. Noda, T. Kobayashi, Y. Shirai, K. Rikitake, T. Yamasaki, S. Hayashi, N. Sakai, H. Seguchi, et al.
Superoxide Production at Phagosomal Cup/Phagosome through {beta}I Protein Kinase C during Fc{gamma}R-Mediated Phagocytosis in Microglia
J. Immunol., October 1, 2004; 173(7): 4582 - 4589.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. W. IJdo and A. C. Mueller
Neutrophil NADPH Oxidase Is Reduced at the Anaplasma phagocytophilum Phagosome
Infect. Immun., September 1, 2004; 72(9): 5392 - 5401.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. A. Carlyon, D. A. Latif, M. Pypaert, P. Lacy, and E. Fikrig
Anaplasma phagocytophilum Utilizes Multiple Host Evasion Mechanisms To Thwart NADPH Oxidase-Mediated Killing during Neutrophil Infection
Infect. Immun., August 1, 2004; 72(8): 4772 - 4783.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. E. Brown, M. Q. Stewart, S. A. Bissonnette, A. E. H. Elia, E. Wilker, and M. B. Yaffe
Distinct Ligand-dependent Roles for p38 MAPK in Priming and Activation of the Neutrophil NADPH Oxidase
J. Biol. Chem., June 25, 2004; 279(26): 27059 - 27068.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. E. Crowther, V. K. Kutala, P. Kuppusamy, J. S. Ferguson, A. A. Beharka, J. L. Zweier, F. X. McCormack, and L. S. Schlesinger
Pulmonary Surfactant Protein A Inhibits Macrophage Reactive Oxygen Intermediate Production in Response to Stimuli by Reducing NADPH Oxidase Activity
J. Immunol., June 1, 2004; 172(11): 6866 - 6874.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-S. Kim, B. A. Diebold, J.-I. Kim, J. Kim, J.-Y. Lee, and J.-B. Park
Rho Is Involved in Superoxide Formation during Phagocytosis of Opsonized Zymosans
J. Biol. Chem., May 14, 2004; 279(20): 21589 - 21597.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. van Bruggen, E. Anthony, M. Fernandez-Borja, and D. Roos
Continuous Translocation of Rac2 and the NADPH Oxidase Component p67phox during Phagocytosis
J. Biol. Chem., March 5, 2004; 279(10): 9097 - 9102.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
F. A. J. van de Loo, M. B. Bennink, O. J. Arntz, R. L. Smeets, E. Lubberts, L. A. B. Joosten, P. L. E. M. van Lent, C. J. J. Coenen-de Roo, S. Cuzzocrea, B. H. Segal, et al.
Deficiency of NADPH Oxidase Components p47phox and gp91phox Caused Granulomatous Synovitis and Increased Connective Tissue Destruction in Experimental Arthritis Models
Am. J. Pathol., October 1, 2003; 163(4): 1525 - 1537.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
T. E. Decoursey
Voltage-Gated Proton Channels and Other Proton Transfer Pathways
Physiol Rev, April 1, 2003; 83(2): 475 - 579.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. M. Voyich, D. E. Sturdevant, K. R. Braughton, S. D. Kobayashi, B. Lei, K. Virtaneva, D. W. Dorward, J. M. Musser, and F. R. DeLeo
Genome-wide protective response used by group A Streptococcus to evade destruction by human polymorphonuclear leukocytes
PNAS, February 18, 2003; 100(4): 1996 - 2001.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. S. Huber-Lang, E. M. Younkin, J. V. Sarma, S. R. McGuire, K. T. Lu, R. F. Guo, V. A. Padgaonkar, J. T. Curnutte, R. Erickson, and P. A. Ward
Complement-Induced Impairment of Innate Immunity During Sepsis
J. Immunol., September 15, 2002; 169(6): 3223 - 3231.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. P. Hoe, R. M. Ireland, F. R. DeLeo, B. B. Gowen, D. W. Dorward, J. M. Voyich, M. Liu, E. H. Burns Jr., D. M. Culnan, A. Bretscher, et al.
Insight into the molecular basis of pathogen abundance: Group A Streptococcus inhibitor of complement inhibits bacterial adherence and internalization into human cells
PNAS, May 28, 2002; 99(11): 7646 - 7651.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. D. Kobayashi, J. M. Voyich, C. L. Buhl, R. M. Stahl, and F. R. DeLeo
Global changes in gene expression by human polymorphonuclear leukocytes during receptor-mediated phagocytosis: Cell fate is regulated at the level of gene expression
PNAS, May 14, 2002; 99(10): 6901 - 6906.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Mott, Y. Rikihisa, and S. Tsunawaki
Effects of Anaplasma phagocytophila on NADPH Oxidase Components in Human Neutrophils and HL-60 Cells
Infect. Immun., March 1, 2002; 70(3): 1359 - 1366.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. J. Mansfield, V. Hinkovska-Galcheva, S. S. Carey, J. A. Shayman, and L. A. Boxer
Regulation of polymorphonuclear leukocyte degranulation and oxidant production by ceramide through inhibition of phospholipase D
Blood, February 15, 2002; 99(4): 1434 - 1441.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
T. Persson, P. Andersson, M. Bodelsson, M. Laurell, J. Malm, and A. Egesten
Bactericidal Activity of Human Eosinophilic Granulocytes against Escherichia coli
Infect. Immun., June 1, 2001; 69(6): 3591 - 3596.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Gallois, J. R. Klein, L.-A. H. Allen, B. D. Jones, and W. M. Nauseef
Salmonella Pathogenicity Island 2-Encoded Type III Secretion System Mediates Exclusion of NADPH Oxidase Assembly from the Phagosomal Membrane
J. Immunol., May 1, 2001; 166(9): 5741 - 5748.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. R. Smith, L. R. Klei, and A. Barchowsky
Arsenite stimulates plasma membrane NADPH oxidase in vascular endothelial cells
Am J Physiol Lung Cell Mol Physiol, March 1, 2001; 280(3): L442 - L449.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Mott and Y. Rikihisa
Human Granulocytic Ehrlichiosis Agent Inhibits Superoxide Anion Generation by Human Neutrophils
Infect. Immun., December 1, 2000; 68(12): 6697 - 6703.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
V. Le Cabec, C. Cols, and I. Maridonneau-Parini
Nonopsonic Phagocytosis of Zymosan and Mycobacterium kansasii by CR3 (CD11b/CD18) Involves Distinct Molecular Determinants and Is or Is Not Coupled with NADPH Oxidase Activation
Infect. Immun., August 1, 2000; 68(8): 4736 - 4745.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
L.-A. H. Allen
Modulating Phagocyte Activation: The Pros and Cons of Helicobacter pylori Virulence Factors
J. Exp. Med., May 1, 2000; 191(9): 1451 - 1454.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. M. Sly, M. Lopez, W. M. Nauseef, and N. E. Reiner
1alpha ,25-Dihydroxyvitamin D3-induced Monocyte Antimycobacterial Activity Is Regulated by Phosphatidylinositol 3-Kinase and Mediated by the NADPH-dependent Phagocyte Oxidase
J. Biol. Chem., September 14, 2001; 276(38): 35482 - 35493.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.