| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115; and
Department of Otorhinolaryngology, Okayama University Medical School, Okayama, Japan
Infection with the helminth parasite Schistosoma mansoni induces a pronounced Th2-type response that is associated with significant IgE production. To better understand how the parasite drives these responses, we investigated the relative roles of proteins and carbohydrates in driving Th2-type and/or IgE responses using a murine model of intranasal sensitization with soluble egg Ags (SEA) of Schistosoma mansoni. We found that repeated intranasal sensitization with soluble egg Ags led to the induction of both total and specific IgE production and nasal eosinophilia. By comparing the responses of mice sensitized with SEA or metaperiodate-treated SEA we were able to demonstrate that carbohydrates on SEA are the major inducers of IgE production and nasal recruitment of eosinophils. Mice sensitized with periodate-treated SEA displayed a significant decrease in both total and specific IgE levels in comparison to mice sensitized with native SEA. Furthermore, sensitization of mice with periodate-treated SEA significantly reduced levels of Ag-specific IgG1, but had no effect on IgG2a production. Nasal lymphocytes from mice sensitized with native SEA, but not with periodate-treated SEA, produced IL-4, IL-5, and IL-10 when restimulated with native SEA in vitro. On the other hand, lymphocytes from mice sensitized with periodate-treated SEA did not produce any of these same cytokines following in vitro restimulation, suggesting that carbohydrates were required for in vivo induction of Th2 response and for that of associated cytokine responses in this model. Lastly, competitive inhibition ELISA showed that although carbohydrates are required for SEA-specific IgE induction, they are not targets of the induced IgE response.
This article has been cited by other articles:
|
|
 |
|
  F. A. Marshall and E. J. Pearce Uncoupling of Induced Protein Processing from Maturation in Dendritic Cells Exposed to a Highly Antigenic Preparation from a Helminth Parasite J. Immunol., December 1, 2008; 181(11): 7562 - 7570. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Jenkins, G. Perona-Wright, and A. S. MacDonald Full Development of Th2 Immunity Requires Both Innate and Adaptive Sources of CD154 J. Immunol., June 15, 2008; 180(12): 8083 - 8092. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Meyer, B. Tefsen, A. Imberty, R. Geyer, and I. van Die The C-type lectin L-SIGN differentially recognizes glycan antigens on egg glycosphingolipids and soluble egg glycoproteins from Schistosoma mansoni Glycobiology, October 1, 2007; 17(10): 1104 - 1119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Jang-Lee, R. S. Curwen, P. D. Ashton, B. Tissot, W. Mathieson, M. Panico, A. Dell, R. A. Wilson, and S. M. Haslam Glycomics Analysis of Schistosoma mansoni Egg and Cercarial Secretions Mol. Cell. Proteomics, September 1, 2007; 6(9): 1485 - 1499. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sun, C. M. Celluzzi, M. Marovich, H. Subramanian, M. Eller, S. Widjaja, D. Palmer, K. Porter, W. Sun, and T. Burgess CD40 Ligand Enhances Dengue Viral Infection of Dendritic Cells: A Possible Mechanism for T Cell-Mediated Immunopathology J. Immunol., November 1, 2006; 177(9): 6497 - 6503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Wuhrer, C. A. M. Koeleman, J. M. Fitzpatrick, K. F. Hoffmann, A. M. Deelder, and C. H. Hokke Gender-specific expression of complex-type N-glycans in schistosomes Glycobiology, October 1, 2006; 16(10): 991 - 1006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. G. Shreffler, R. R. Castro, Z. Y. Kucuk, Z. Charlop-Powers, G. Grishina, S. Yoo, A. W. Burks, and H. A. Sampson The Major Glycoprotein Allergen from Arachis hypogaea, Ara h 1, Is a Ligand of Dendritic Cell-Specific ICAM-Grabbing Nonintegrin and Acts as a Th2 Adjuvant In Vitro J. Immunol., September 15, 2006; 177(6): 3677 - 3685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Hassan, Y. Zhang, C. R. Engwerda, P. M. Kaye, H. Sharp, and Q. D. Bickle The Schistosoma mansoni Hepatic Egg Granuloma Provides a Favorable Microenvironment for Sustained Growth of Leishmania donovani Am. J. Pathol., September 1, 2006; 169(3): 943 - 953. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Martinez-Maza Overview of the Immune System and Use of Immunologic Biomarkers to Interrogate Cancer Etiology Am. Assoc. Cancer Res. Educ. Book, April 1, 2006; 2006(1): 31 - 34. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Meyer, E. van Liempt, A. Imberty, Y. van Kooyk, H. Geyer, R. Geyer, and I. van Die DC-SIGN Mediates Binding of Dendritic Cells to Authentic Pseudo-LewisY Glycolipids of Schistosoma mansoni Cercariae, the First Parasite-specific Ligand of DC-SIGN J. Biol. Chem., November 11, 2005; 280(45): 37349 - 37359. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Jenkins, J. P. Hewitson, S. Ferret-Bernard, and A. P. Mountford Schistosome larvae stimulate macrophage cytokine production through TLR4-dependent and -independent pathways Int. Immunol., November 1, 2005; 17(11): 1409 - 1418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Atochina and D. Harn LNFPIII/LeX-Stimulated Macrophages Activate Natural Killer Cells via CD40-CD40L Interaction Clin. Vaccine Immunol., September 1, 2005; 12(9): 1041 - 1049. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Raes, L. Brys, B. K. Dahal, J. Brandt, J. Grooten, F. Brombacher, G. Vanham, W. Noel, P. Bogaert, T. Boonefaes, et al. Macrophage galactose-type C-type lectins as novel markers for alternatively activated macrophages elicited by parasitic infections and allergic airway inflammation J. Leukoc. Biol., March 1, 2005; 77(3): 321 - 327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Donnelly, S. M. O'Neill, M. Sekiya, G. Mulcahy, and J. P. Dalton Thioredoxin Peroxidase Secreted by Fasciola hepatica Induces the Alternative Activation of Macrophages Infect. Immun., January 1, 2005; 73(1): 166 - 173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tawill, L. Le Goff, F. Ali, M. Blaxter, and J. E. Allen Both Free-Living and Parasitic Nematodes Induce a Characteristic Th2 Response That Is Dependent on the Presence of Intact Glycans Infect. Immun., January 1, 2004; 72(1): 398 - 407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Gyetko, S. Sud, and S. W. Chensue Urokinase-Deficient Mice Fail To Generate a Type 2 Immune Response following Schistosomal Antigen Challenge Infect. Immun., January 1, 2004; 72(1): 461 - 467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Thomas, M. R. Carter, O. Atochina, A. A. Da'Dara, D. Piskorska, E. McGuire, and D. A. Harn Maturation of Dendritic Cell 2 Phenotype by a Helminth Glycan Uses a Toll-Like Receptor 4-Dependent Mechanism J. Immunol., December 1, 2003; 171(11): 5837 - 5841. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Vervelde, N. Bakker, F. N.J. Kooyman, A. W.C.A. Cornelissen, C. M.C. Bank, A. K. Nyame, R. D. Cummings, and I. van Die Vaccination-induced protection of lambs against the parasitic nematode Haemonchus contortus correlates with high IgG antibody responses to the LDNF glycan antigen Glycobiology, November 1, 2003; 13(11): 795 - 804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Schramm, F. H. Falcone, A. Gronow, K. Haisch, U. Mamat, M. J. Doenhoff, G. Oliveira, J. Galle, C. A. Dahinden, and H. Haas Molecular Characterization of an Interleukin-4-inducing Factor from Schistosoma mansoni Eggs J. Biol. Chem., May 9, 2003; 278(20): 18384 - 18392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Appelmelk, I. van Die, S. J. van Vliet, C. M. J. E. Vandenbroucke-Grauls, T. B. H. Geijtenbeek, and Y. van Kooyk Cutting Edge: Carbohydrate Profiling Identifies New Pathogens That Interact with Dendritic Cell-Specific ICAM-3-Grabbing Nonintegrin on Dendritic Cells J. Immunol., February 15, 2003; 170(4): 1635 - 1639. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Straw, A. S. MacDonald, E. Y. Denkers, and E. J. Pearce CD154 Plays a Central Role in Regulating Dendritic Cell Activation During Infections That Induce Th1 or Th2 Responses J. Immunol., January 15, 2003; 170(2): 727 - 734. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Liu, Q. Liu, J. Pesce, J. Whitmire, M. J. Ekkens, A. Foster, J. VanNoy, A. H. Sharpe, J. F. Urban Jr., and W. C. Gause Nippostrongylus brasiliensis Can Induce B7-Independent Antigen-Specific Development of IL-4-Producing T Cells from Naive CD4 T Cells In Vivo J. Immunol., December 15, 2002; 169(12): 6959 - 6968. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Faveeuw, V. Angeli, J. Fontaine, C. Maliszewski, A. Capron, L. Van Kaer, M. Moser, M. Capron, and F. Trottein Antigen Presentation by CD1d Contributes to the Amplification of Th2 Responses to Schistosoma mansoni Glycoconjugates in Mice J. Immunol., July 15, 2002; 169(2): 906 - 912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rodriguez-Sosa, A. R. Satoskar, R. Calderon, L. Gomez-Garcia, R. Saavedra, R. Bojalil, and L. I. Terrazas Chronic Helminth Infection Induces Alternatively Activated Macrophages Expressing High Levels of CCR5 with Low Interleukin-12 Production and Th2-Biasing Ability Infect. Immun., July 1, 2002; 70(7): 3656 - 3664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Korten, L. Volkmann, M. Saeftel, K. Fischer, M. Taniguchi, B. Fleischer, and A. Hoerauf Expansion of NK Cells with Reduction of Their Inhibitory Ly-49A, Ly-49C, and Ly-49G2 Receptor-Expressing Subsets in a Murine Helminth Infection: Contribution to Parasite Control J. Immunol., May 15, 2002; 168(10): 5199 - 5206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. MacDonald and E. J. Pearce Cutting Edge: Polarized Th Cell Response Induction by Transferred Antigen-Pulsed Dendritic Cells Is Dependent on IL-4 or IL-12 Production by Recipient Cells J. Immunol., April 1, 2002; 168(7): 3127 - 3130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. MacDonald, M. I. Araujo, and E. J. Pearce Immunology of Parasitic Helminth Infections Infect. Immun., February 1, 2002; 70(2): 427 - 433. [Full Text] [PDF]
|
|
|
|
|
|
L. I. Terrazas, K. L. Walsh, D. Piskorska, E. McGuire, and D. A. Harn Jr. The Schistosome Oligosaccharide Lacto-N-neotetraose Expands Gr1+ Cells That Secrete Anti-inflammatory Cytokines and Inhibit Proliferation of Naive CD4+ Cells: A Potential Mechanism for Immune Polarization in Helminth Infections J. Immunol., November 1, 2001; 167(9): 5294 - 5303. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. OKANO, M. AZUMA, T. YOSHINO, H. HATTORI, M. NAKADA, A. R. SATOSKAR, D. A. HARN Jr, E. NAKAYAMA, T. AKAGI, and K. NISHIZAKI Differential Role of CD80 and CD86 Molecules in the Induction and the Effector Phases of Allergic Rhinitis in Mice Am. J. Respir. Crit. Care Med., October 15, 2001; 164(8): 1501 - 1507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. MacDonald, A. D. Straw, B. Bauman, and E. J. Pearce CD8- Dendritic Cell Activation Status Plays an Integral Role in Influencing Th2 Response Development J. Immunol., August 15, 2001; 167(4): 1982 - 1988. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. King, M. Connelly, M. P. Alpers, M. Bockarie, and J. W. Kazura Transmission Intensity Determines Lymphocyte Responsiveness and Cytokine Bias in Human Lymphatic Filariasis J. Immunol., June 15, 2001; 166(12): 7427 - 7436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E.T.A. Marques Jr., Y. Ichikawa, M. Strand, J.T. August, G.W. Hart, and R.L. Schnaar Fucosyltransferases in Schistosoma mansoni development Glycobiology, March 1, 2001; 11(3): 249 - 259. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
