HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Poljak, L. Articles by Siebenlist, U. Search for Related Content PubMed PubMed Citation Articles by Poljak, L. Articles by Siebenlist, U.

Distinct Activities of p52/NF-B Required for Proper Secondary Lymphoid Organ Microarchitecture: Functions Enhanced by Bcl-3

Ljiljana Poljak^*, Louise Carlson^*, Kirk Cunningham^*, Marie H. Kosco-Vilbois and Ulrich Siebenlist^1,*

^* Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; and Serono Pharmaceutical Research Institute, Plan-les-Ouates, Geneva, Switzerland

Mice rendered deficient in p52, a subunit of NF-B, or in Bcl-3, an IB-related regulator that associates with p52 homodimers, share defects in the microarchitecture of secondary lymphoid organs. The mutant mice are impaired in formation of B cell follicles and are unable to form proper follicular dendritic cell (FDC) networks upon antigenic challenge. The defects in formation of B cell follicles may be attributed, at least in part, to impaired production of the B lymphocyte chemoattractant (BLC) chemokine, possibly a result of defective FDCs. The p52- and Bcl-3-deficient mice exhibit additional defects within the splenic marginal zone, including reduced numbers of metallophilic macrophages, reduced deposition of the laminin-ß2 chain and impaired expression of a mucosal addressin marker on sinus-lining cells. Whereas p52-deficient mice are severely defective in all of these aspects, Bcl-3-deficient mice are only partially defective. We determined that FDCs or other non-hemopoietic cells that underlie FDCs are intrinsically impaired in p52-deficient mice. Adoptive transfers of wild-type bone marrow into p52-deficient mice failed to restore FDC networks or follicles. The transfers did restore metallophilic macrophages to the marginal zone, however. Together, the results suggest that p52 carries out functions essential for a proper splenic microarchitecture in both hemopoietic and non-hemopoietic cells and that Bcl-3 is important in enhancing these essential activities of p52.

This article has been cited by other articles:

				Y. Chen, T. Pikkarainen, O. Elomaa, R. Soininen, T. Kodama, G. Kraal, and K. Tryggvason Defective Microarchitecture of the Spleen Marginal Zone and Impaired Response to a Thymus-Independent Type 2 Antigen in Mice Lacking Scavenger Receptors MARCO and SR-A J. Immunol., December 15, 2005; 175(12): 8173 - 8180. [Abstract] [Full Text] [PDF]

				L. A. Lyakh, M. Sanford, S. Chekol, H. A. Young, and A. B. Roberts TGF-{beta} and Vitamin D3 Utilize Distinct Pathways to Suppress IL-12 Production and Modulate Rapid Differentiation of Human Monocytes into CD83+ Dendritic Cells J. Immunol., February 15, 2005; 174(4): 2061 - 2070. [Abstract] [Full Text] [PDF]

				D. Carragher, R. Johal, A. Button, A. White, A. Eliopoulos, E. Jenkinson, G. Anderson, and J. Caamano A Stroma-Derived Defect in NF-{kappa}B2-/- Mice Causes Impaired Lymph Node Development and Lymphocyte Recruitment J. Immunol., August 15, 2004; 173(4): 2271 - 2279. [Abstract] [Full Text] [PDF]

				B. Ge, O. Li, P. Wilder, A. Rizzino, and T. W. McKeithan NF-{kappa}B Regulates BCL3 Transcription in T Lymphocytes Through an Intronic Enhancer J. Immunol., October 15, 2003; 171(8): 4210 - 4218. [Abstract] [Full Text] [PDF]

				C. M. Witt, W.-J. Won, V. Hurez, and C. A. Klug Notch2 Haploinsufficiency Results in Diminished B1 B Cells and a Severe Reduction in Marginal Zone B Cells J. Immunol., September 15, 2003; 171(6): 2783 - 2788. [Abstract] [Full Text] [PDF]

				N. Watanabe, S. Wachi, and T. Fujita Identification and Characterization of BCL-3-binding Protein: IMPLICATIONS FOR TRANSCRIPTION AND DNA REPAIR OR RECOMBINATION J. Biol. Chem., July 3, 2003; 278(28): 26102 - 26110. [Abstract] [Full Text] [PDF]

				V. Sivakumar, K. J.L. Hammond, N. Howells, K. Pfeffer, and F. Weih Differential Requirement for Rel/Nuclear Factor {kappa}B Family Members in Natural Killer T Cell Development J. Exp. Med., June 16, 2003; 197(12): 1613 - 1621. [Abstract] [Full Text] [PDF]

				J. R. Muller and U. Siebenlist Lymphotoxin beta Receptor Induces Sequential Activation of Distinct NF-kappa B Factors via Separate Signaling Pathways J. Biol. Chem., March 28, 2003; 278(14): 12006 - 12012. [Abstract] [Full Text] [PDF]

				A. Nijnik, R. Mott, D. P. Kwiatkowski, and I. A. Udalova Comparing the fine specificity of DNA binding by NF-{kappa}B p50 and p52 using principal coordinates analysis Nucleic Acids Res., March 1, 2003; 31(5): 1497 - 1501. [Abstract] [Full Text] [PDF]

				L. A. Miosge, J. Blasioli, M. Blery, and C. C. Goodnow Analysis of an Ethylnitrosourea-generated Mouse Mutation Defines a Cell Intrinsic Role of Nuclear Factor {kappa}B2 in Regulating Circulating B Cell Numbers J. Exp. Med., October 21, 2002; 196(8): 1113 - 1119. [Abstract] [Full Text] [PDF]

				C. R. Engwerda, M. Ato, S. E. J. Cotterell, T. L. Mynott, A. Tschannerl, P. M. A. Gorak-Stolinska, and P. M. Kaye A Role for Tumor Necrosis Factor-{alpha} in Remodeling the Splenic Marginal Zone during Leishmania donovani Infection Am. J. Pathol., August 1, 2002; 161(2): 429 - 437. [Abstract] [Full Text] [PDF]

				D. S. Weih, Z. B. Yilmaz, and F. Weih Essential Role of RelB in Germinal Center and Marginal Zone Formation and Proper Expression of Homing Chemokines J. Immunol., August 15, 2001; 167(4): 1909 - 1919. [Abstract] [Full Text] [PDF]

				M. Wysocka, S. Robertson, H. Riemann, J. Caamano, C. Hunter, A. Mackiewicz, L. J. Montaner, G. Trinchieri, and C. L. Karp IL-12 Suppression During Experimental Endotoxin Tolerance: Dendritic Cell Loss and Macrophage Hyporesponsiveness J. Immunol., June 15, 2001; 166(12): 7504 - 7513. [Abstract] [Full Text] [PDF]

				P. Hjelmström Lymphoid neogenesis: de novo formation of lymphoid tissue in chronic inflammation through expression of homing chemokines J. Leukoc. Biol., March 1, 2001; 69(3): 331 - 339. [Abstract] [Full Text]

				J. Caamano, C. Tato, G. Cai, E. N. Villegas, K. Speirs, L. Craig, J. Alexander, and C. A. Hunter Identification of a Role for NF-{kappa}B2 in the Regulation of Apoptosis and in Maintenance of T Cell-Mediated Immunity to Toxoplasma gondii J. Immunol., November 15, 2000; 165(10): 5720 - 5728. [Abstract] [Full Text] [PDF]