| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
*
Microbiology and Immunology,
Medicine, and
Anatomy, Division of Immunobiology, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, VA 23298
The observation that follicular dendritic cells (FDC) reduce
apoptosis in B cells prompted the hypothesis that FDC might enhance
tumor cell survival by protecting malignant B cells from apoptotic
death. To test this notion, apoptosis was induced in B cell lymphomas
by anti-Fas or various antineoplastic agents in the presence and
absence of FDC. Apoptosis was detected and quantified by TUNEL
analysis. Induction of apoptosis with anti-Fas, etoposide,
cyclophosphamide, and busulfan was markedly antagonized by FDC at FDC
to B cell ratios of 
1:16. For example, treatment with 10 ng/ml
anti-Fas caused 60–90% of A20 cells to undergo apoptosis in
6 h, whereas addition of FDC reduced apoptosis to background
levels (3–15%). Similarly, treatment with busulfan induced apoptosis
in 55–80% of A20 cells, whereas addition of FDC reduced B cell death
to 
15%; moreover, depletion of FDC abrogated the protective actions.
In contrast, the apoptosis-inducing effect of Adriamycin was not
reversed by FDC. The ability to block apoptosis induced by anti-Fas
or busulfan was not limited to A20 but was observed in four other
malignant pre-B cell or B cell lines. The mechanism by which FDC spare
malignant B cells from apoptosis did not involve alterations in levels
of Bcl-2, Bcl-XL, or Bax. Collectively, these data raise
the possibility that FDC may enhance tumor cell survival by protecting
malignant B cells against apoptosis induced by anti-Fas and some
but not all chemotherapeutic agents.
This article has been cited by other articles:
|
|
 |
|
  M. E. M. El Shikh, R. M. El Sayed, A. K. Szakal, and J. G. Tew T-Independent Antibody Responses to T-Dependent Antigens: A Novel Follicular Dendritic Cell-Dependent Activity J. Immunol., March 15, 2009; 182(6): 3482 - 3491. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Nohara, K. Ao, Y. Miyamoto, T. Suzuki, S. Imaizumi, Y. Tateishi, S. Omura, C. Tohyama, and T. Kobayashi Arsenite-Induced Thymus Atrophy is Mediated by Cell Cycle Arrest: A Characteristic Downregulation of E2F-Related Genes Revealed by a Microarray Approach Toxicol. Sci., February 1, 2008; 101(2): 226 - 238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. M. El Shikh, R. M. El Sayed, Y. Wu, A. K. Szakal, and J. G. Tew TLR4 on Follicular Dendritic Cells: An Activation Pathway That Promotes Accessory Activity J. Immunol., October 1, 2007; 179(7): 4444 - 4450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Probin, Y. Wang, A. Bai, and D. Zhou Busulfan Selectively Induces Cellular Senescence but Not Apoptosis in WI38 Fibroblasts via a p53-Independent but Extracellular Signal-Regulated Kinase-p38 Mitogen-Activated Protein Kinase-Dependent Mechanism J. Pharmacol. Exp. Ther., November 1, 2006; 319(2): 551 - 560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wen, S. A. Shinton, R. R. Hardy, and K. Hayakawa Association of B-1 B Cells with Follicular Dendritic Cells in Spleen J. Immunol., June 1, 2005; 174(11): 6918 - 6926. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Aydar, P. Balogh, J. G. Tew, and A. K. Szakal Altered Regulation of Fc{gamma}RII on Aged Follicular Dendritic Cells Correlates with Immunoreceptor Tyrosine-Based Inhibition Motif Signaling in B Cells and Reduced Germinal Center Formation J. Immunol., December 1, 2003; 171(11): 5975 - 5987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Meng, Y. Wang, G. Van Zant, and D. Zhou Ionizing Radiation and Busulfan Induce Premature Senescence in Murine Bone Marrow Hematopoietic Cells Cancer Res., September 1, 2003; 63(17): 5414 - 5419. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Muscarella and S. E. Bloom Cross-linking of Surface IgM in the Burkitt's Lymphoma Cell Line ST486 Provides Protection against Arsenite- and Stress-induced Apoptosis That Is Mediated by ERK and Phosphoinositide 3-Kinase Signaling Pathways J. Biol. Chem., January 31, 2003; 278(6): 4358 - 4367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Estes, B. F. Keele, K. Tenner-Racz, P. Racz, M. A. Redd, T. C. Thacker, Y. Jiang, M. J. Lloyd, S. Gartner, and G. F. Burton Follicular Dendritic Cell-Mediated Up-Regulation of CXCR4 Expression on CD4 T Cells and HIV Pathogenesis J. Immunol., September 1, 2002; 169(5): 2313 - 2322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Payet-Jamroz, S. L. T. Helm, J. Wu, M. Kilmon, M. Fakher, A. Basalp, J. G. Tew, A. K. Szakal, N. Noben-Trauth, and D. H. Conrad Suppression of IgE Responses in CD23-Transgenic Animals Is Due to Expression of CD23 on Nonlymphoid Cells J. Immunol., April 15, 2001; 166(8): 4863 - 4869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. T. Eichhorst, S. Müerköster, M. A. Weigand, and P. H. Krammer The Chemotherapeutic Drug 5-Fluorouracil Induces Apoptosis in Mouse Thymocytes in Vivo via Activation of the CD95(APO-1/Fas) System Cancer Res., January 1, 2001; 61(1): 243 - 248. [Abstract] [Full Text]
|
|
|
|
|
|
F. Zhang, M. Rincon, R. A. Flavell, and T. M. Aune Defective Th Function Induced by a Dominant-Negative cAMP Response Element Binding Protein Mutation Is Reversed by Bcl-2 J. Immunol., August 15, 2000; 165(4): 1762 - 1770. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
