HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dennis, G. Articles by Cooper, M. D. Search for Related Content PubMed PubMed Citation Articles by Dennis, G., Jr. Articles by Cooper, M. D.

Characterization of Paired Ig-Like Receptors in Rats^{1 ,2}

Glynn Dennis, Jr.^*,, Robert P. Stephan^*, Hiromi Kubagawa^*, and Max D. Cooper^{3,*,,§,¶,||}

^* Division of Developmental and Clinical Immunology and Departments of Microbiology, Pathology, ^§ Pediatrics, and ^¶ Medicine and ^|| Howard Hughes Medical Institute, University of Alabama, Birmingham, AL 35294

To explore the phylogenetic history of the murine paired Ig-like receptors of activating (PIR-A) and inhibitory (PIR-B) types, we isolated PIR homologues from a rat splenocyte cDNA library. The rat (ra) PIR-A and raPIR-B cDNA sequences predict transmembrane proteins with six highly conserved extracellular Ig-like domains and distinctive membrane proximal, transmembrane, and cytoplasmic regions. The raPIR-B cytoplasmic region contains prototypic inhibitory motifs, whereas raPIR-A features a charged transmembrane region and a short cytoplasmic tail. Southern blot analysis predicts the presence of multiple Pira genes and a single Pirb gene in the rat genome. Although raPIR-A and raPIR-B are coordinately expressed by myeloid cells, analysis of mRNA detected unpaired expression of raPIR-A by B cells and raPIR-B by NK cells. Collectively, these findings indicate that the structural hallmarks of the Pir gene family are conserved in rats and mice, yet suggest divergence of PIR regulatory elements during rodent speciation.

This article has been cited by other articles:

				S. Kollnberger, L. A. Bird, M. Roddis, C. Hacquard-Bouder, H. Kubagawa, H. C. Bodmer, M. Breban, A. J. McMichael, and P. Bowness HLA-B27 Heavy Chain Homodimers Are Expressed in HLA-B27 Transgenic Rodent Models of Spondyloarthritis and Are Ligands for Paired Ig-Like Receptors J. Immunol., August 1, 2004; 173(3): 1699 - 1710. [Abstract] [Full Text] [PDF]

				G. Dennis Jr., H. Kubagawa, and M. D. Cooper Paired Ig-like receptor homologs in birds and mammals share a common ancestor with mammalian Fc receptors PNAS, November 21, 2000; 97(24): 13245 - 13250. [Abstract] [Full Text] [PDF]