HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by He, X. Articles by Stuart, J. M. Search for Related Content PubMed PubMed Citation Articles by He, X. Articles by Stuart, J. M.

Prostaglandin E₂ Selectively Inhibits Human CD4⁺ T Cells Secreting Low Amounts of Both IL-2 and IL-4¹

Xiaowen He^2, and John M. Stuart^*,

^* Research Service 151, Veterans Administration Medical Center, and Department of Medicine, University of Tennessee, Memphis, TN 38104

PGE₂ is a potent inflammatory mediator with profound immune regulatory actions. The present study examined the effects of PGE₂ on the activation/proliferation of CD4⁺ T cells using 37 cloned CD4⁺ T cell lines. Ten T cell clones sensitive to PGE₂ and 10 T cell clones resistant to PGE_2, as measured by proliferation in response to anti-CD3 Ab, were selected for comparison. It was found that the PGE₂-sensitive T cells were characterized by low production (<200 pg/ml) of both IL-2 and IL-4, while PGE₂-resistant T cells secreted high levels (>1000 pg/ml) of IL-2, IL-4, or both. The roles of IL-2 and IL-4 were confirmed by the finding that addition of exogenous lymphokines could restore PGE₂-inhibited proliferation, and PGE₂-resistant Th1-, Th2-, and Th0-like clones became PGE₂ sensitive when IL-2, IL-4, or both were removed using Abs specific for the respective lymphokines. In addition, we showed that the CD45RA expression in PGE₂-sensitive T cells was significantly lower than that in PGE₂-resistant cells (mean intensity, 1.2 ± 0.6 vs 7.8 ± 5.7; p = 0.001). In contrast, CD45RO expression in PGE₂-sensitive T cells was significantly higher that that in PGE₂-resistant cells (mean intensity, 55.7 ± 15.1 vs 33.4 ± 12.9; p = 0.02). In summary, PGE₂ predominantly suppressed CD45RA^-RO⁺ CD4⁺ T cells with low secretion of both IL-2 and IL-4.

This article has been cited by other articles:

				C. Chizzolini, R. Chicheportiche, M. Alvarez, C. de Rham, P. Roux-Lombard, S. Ferrari-Lacraz, and J.-M. Dayer Prostaglandin E2 synergistically with interleukin-23 favors human Th17 expansion Blood, November 1, 2008; 112(9): 3696 - 3703. [Abstract] [Full Text] [PDF]

				P. Comoli, F. Ginevri, R. Maccario, M. A. Avanzini, M. Marconi, A. Groff, A. Cometa, M. Cioni, L. Porretti, W. Barberi, et al. Human mesenchymal stem cells inhibit antibody production induced in vitro by allostimulation Nephrol. Dial. Transplant., April 1, 2008; 23(4): 1196 - 1202. [Abstract] [Full Text] [PDF]

				R. Kim, M. Emi, K. Tanabe, and K. Arihiro Tumor-Driven Evolution of Immunosuppressive Networks during Malignant Progression Cancer Res., June 1, 2006; 66(11): 5527 - 5536. [Abstract] [Full Text] [PDF]

				A. M. Holm, P. Aukrust, E. M. Aandahl, F. Muller, K. Tasken, and S. S. Froland Impaired Secretion of IL-10 by T Cells from Patients with Common Variable Immunodeficiency-Involvement of Protein Kinase A Type I J. Immunol., June 1, 2003; 170(11): 5772 - 5777. [Abstract] [Full Text] [PDF]

				H. Yoneda, K. Miura, H. Matsushima, K. Sugi, T. Murakami, K. Ouchi, K. Yamashita, H. Itoh, T. Nakazawa, M. Suzuki, et al. Aspirin inhibits Chlamydia pneumoniae-induced NF-{kappa}B activation, cyclo-oxygenase-2 expression and prostaglandin E2 synthesis and attenuates chlamydial growth J. Med. Microbiol., May 1, 2003; 52(5): 409 - 415. [Abstract] [Full Text] [PDF]

				A. Suarez, L. Mozo, and C. Gutierrez Generation of CD4+CD45RA+ Effector T Cells by Stimulation in the Presence of Cyclic Adenosine 5'-Monophosphate- Elevating Agents J. Immunol., August 1, 2002; 169(3): 1159 - 1167. [Abstract] [Full Text] [PDF]

				X. Duhant, L. Schandene, C. Bruyns, N. S. Gonzalez, M. Goldman, J.-M. Boeynaems, and D. Communi Extracellular Adenine Nucleotides Inhibit the Activation of Human CD4+ T Lymphocytes J. Immunol., July 1, 2002; 169(1): 15 - 21. [Abstract] [Full Text] [PDF]

				X. He, C. M. Weyand, J. J. Goronzy, W. Zhong, and J. M. Stuart Bi-directional modulation of T cell-dependent antibody production by prostaglandin E2 Int. Immunol., January 1, 2002; 14(1): 69 - 77. [Abstract] [Full Text] [PDF]

				K. L. Clark, Z. Zeng, A. L. Langford, S. M. Bowen, and S. C. Todd PGRL Is a Major CD81-Associated Protein on Lymphocytes and Distinguishes a New Family of Cell Surface Proteins J. Immunol., November 1, 2001; 167(9): 5115 - 5121. [Abstract] [Full Text] [PDF]

				W. H. Loomis, S. Namiki, D. B. Hoyt, and W. G. Junger Hypertonicity rescues T cells from suppression by trauma-induced anti-inflammatory mediators Am J Physiol Cell Physiol, September 1, 2001; 281(3): C840 - C848. [Abstract] [Full Text] [PDF]