HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Korten, S. Articles by Schmidt, R. E. Search for Related Content PubMed PubMed Citation Articles by Korten, S. Articles by Schmidt, R. E.

Altered Donor and Recipient Ly49⁺ NK Cell Subsets in Allogeneic H-2^d H-2^b and H-2^b H-2^d Bone Marrow Chimeras¹

Department of Clinical Immunology, Medical School Hannover, Hannover, Germany

NK cells reject non-self hematopoietic bone marrow (BM) grafts via Ly49 receptor-mediated MHC class I-specific recognition and calibration of receptor expression levels. In this paper we investigated how Ly49⁺ subset frequencies were regulated dependent on MHC class I expression. The development of donor and host Ly49A⁺ (recognizes H-2D^d and H-2D^k ligands) and Ly49C/I⁺ (Ly49C^BALB/c recognizes H-2K^b, H-2K^d, and H-2D^d, and Ly49C^B6 recognizes only H-2K^b) NK cell frequencies were monitored for 120 days in murine-mixed allogeneic BM chimeras. C57BL/6 (H-2^b) BM was transplanted into BALB/c (H-2^d) mice and vice versa. Peripheral NK cell populations were examined every 5 days. Chimerism was found to be stable with 80–90% donor NK cells. In contrast to syngeneic controls reexpressing pretransplant patterns, donor and host NK cells revealed new and mainly reduced subset frequencies 55 days after allogeneic transplantation. Recipient NK cells acquired these later than donor NK cells. In H-2^d H-2^b chimeras Ly49A⁺, Ly49C/I⁺, and Ly49A⁺/Ly49C/I⁺ proportions were mainly diminished upon interaction with cognate ligands. Also in H-2^b H-2^d chimeras, Ly49A⁺ and Ly49A⁺/Ly49C/I⁺ subsets were reduced, but there was a transient normalization of Ly49C/I⁺ proportions in the noncognate host. After 120 days all subsets were reduced. Therefore, down-regulation of developing Ly49A⁺ and Ly49C/I⁺ chimeric NK cell frequencies by cognate ligands within 7–8 wk after BM transplantation may be important for successful engraftment.

This article has been cited by other articles:

				K. Wendt, E. Wilk, S. Buyny, J. Buer, R. E. Schmidt, and R. Jacobs Gene and protein characteristics reflect functional diversity of CD56dim and CD56bright NK cells J. Leukoc. Biol., December 1, 2006; 80(6): 1529 - 1541. [Abstract] [Full Text] [PDF]

				Y. Huang, F. Rezzoug, H. Xu, P. M. Chilton, C. L. Schanie, I. Fugier-Vivier, and S. T. Ildstad NK Cells Play a Critical Role in the Regulation of Class I-Deficient Hemopoietic Stem Cell Engraftment: Evidence for NK Tolerance Correlates with Receptor Editing J. Immunol., September 15, 2005; 175(6): 3753 - 3761. [Abstract] [Full Text] [PDF]

				S. Korten, L. Volkmann, M. Saeftel, K. Fischer, M. Taniguchi, B. Fleischer, and A. Hoerauf Expansion of NK Cells with Reduction of Their Inhibitory Ly-49A, Ly-49C, and Ly-49G2 Receptor-Expressing Subsets in a Murine Helminth Infection: Contribution to Parasite Control J. Immunol., May 15, 2002; 168(10): 5199 - 5206. [Abstract] [Full Text] [PDF]

				F. Heusohn, G. Wirries, R. E. Schmidt, and J. E. Gessner The Pmed1 Gene Promoter of Human Fc{gamma}RIIIA Can Function as a NK/T Cell-Specific Restriction Element, Which Involves Binding of Sp1 Transcription Factor J. Immunol., March 15, 2002; 168(6): 2857 - 2864. [Abstract] [Full Text] [PDF]