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CUTTING EDGE |
Regulated Germline Transcripts Are Expressed from 2a Transgenes Independently of the Heavy Chain 3' Enhancers1
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109
Several results indicate that transcriptional enhancers lying 3'
of the C
gene regulate RNA expression and switch recombination of
heavy chain genes. To investigate this regulation we prepared
transgenic mice with a 10.5-kb transgene that included the germline
form of the murine 
2 gene, including promoter, I, S, and C
regions. RNA was expressed from these 2a transgenes with correct
IFN- regulation, in spite of the fact that they lacked the 3'
enhancers. This RNA expression was independent of insertion site and
dependent on copy number, indicating that the 2a gene includes locus
control region-like elements. Addition of either a cassette containing
3' enhancer DNase I hypersensitive sites 1, 2, 3B, and 4 or the
intronic µ enhancer increased transcription from the 2a transgene
by 
75-fold in B cells. However, this increased transcription was not
responsive to IFN- treatment of the transgenic B
cells.
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