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Meakins-Christie Laboratories, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada
To test the hypothesis that CD8+ T cells may suppress
the allergen-induced late airway response (LAR) and airway
eosinophilia, we examined the effect of administration of Ag-primed
CD8+ T cells on allergic airway responses, bronchoalveolar
lavage (BAL) leukocytes, and mRNA expression for cytokines (IL-4, IL-5,
and IFN-
) in OVA-sensitized Brown Norway rats. On day 12
postsensitization to OVA, test rats were administered 2 million
CD8+ T cells i.p. isolated from either the cervical lymph
nodes (LN group; n = 8) or the spleen (Spl group;
n = 6) of sensitized donors. On day 14, test rats
were challenged with aerosolized OVA. Control rats were administered
PBS i.p. on day 12, and challenged with OVA (n =
10) or BSA (n = 6) on day 14. The lung resistance
was measured for 8 h after challenge. BAL was performed at 8 h.
Cytospin slides of BAL were analyzed for major basic protein by
immunostaining and for cytokine mRNA by in situ hybridization. The LAR
was significantly less in the LN group (1.8 ± 0.5 U;
p < 0.01) and BSA controls (1.4 ± 0.7;
p < 0.01), but not in the Spl group (6.7 ±
2.2), compared with that in OVA controls (8.1 ± 1.8). In BAL, the
number of major basic protein-positive cells was lower in the LN and
Spl groups compared with OVA controls (p < 0.05
and p < 0.01). IL-4- and IL-5-positive cells were
decreased in the LN group compared with the OVA controls
(p < 0.01). INF--positive cells were increased
in the LN and Spl groups compared with the OVA controls
(p < 0.01). Serum OVA-specific IgE levels were
unaffected by CD8+ T cell transfers. These results indicate
that Ag-primed CD8+ T cells have a potent suppressive
effect on LAR.
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