| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
,
*
Division of Rheumatology, Immunology and Allergy, Brigham and Women’s Hospital, and Harvard Medical School, Boston, MA 02115;
Procept, Cambridge, MA; and
Aquila Biopharmaceuticals, Framingham, MA 01702
CD1 is a family of cell-surface molecules capable of presenting microbial lipid Ags to specific T cells. Here we describe the CD1 gene family of the guinea pig (Cavia porcellus). Eight distinct cDNA clones corresponding to CD1 transcripts were isolated from a guinea pig thymocyte cDNA library and completely sequenced. The guinea pig CD1 proteins predicted by translation of the cDNAs included four that can be classified as homologues of human CD1b, three that were homologues of human CD1c, and a single CD1e homologue. These guinea pig CD1 protein sequences contain conserved amino acid residues and hydrophobic domains within the putative Ag binding pocket. A mAb specific for human CD1b cross-reacted with multiple guinea pig CD1 isoforms, thus allowing direct analysis of the structure and expression of at least a subset of guinea pig CD1 proteins. Cell-surface expression of CD1 was detected on cortical thymocytes, dermal dendritic cells in the skin, follicular dendritic cells of lymph nodes, and in the B cell regions within the lymph nodes and spleen. CD1 proteins were also detected on a subset of PBMCs consistent with expression on circulating B cells. This distribution of CD1 staining in guinea pig tissues was thus similar to that seen in other mammals. These data provide the foundation for the development of the guinea pig as an animal model to study the in vivo function of CD1.
This article has been cited by other articles:
|
|
 |
|
  I. Van Rhijn, A. P. Koets, J. S. Im, D. Piebes, F. Reddington, G. S. Besra, S. A. Porcelli, W. van Eden, and V. P. M. G. Rutten The Bovine CD1 Family Contains Group 1 CD1 Proteins, but No Functional CD1d. J. Immunol., April 15, 2006; 176(8): 4888 - 4893. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Miller, C. Wang, E. Parisini, R. D. Coletta, R. M. Goto, S. Y. Lee, D. C. Barral, M. Townes, C. Roura-Mir, H. L. Ford, et al. From The Cover: Characterization of two avian MHC-like genes reveals an ancient origin of the CD1 family PNAS, June 14, 2005; 102(24): 8674 - 8679. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Patton, T. C. McGuire, M. T. Hines, R. H. Mealey, and S. A. Hines Rhodococcus equi-Specific Cytotoxic T Lymphocytes in Immune Horses and Development in Asymptomatic Foals Infect. Immun., April 1, 2005; 73(4): 2083 - 2093. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Dascher, K. Hiromatsu, X. Xiong, C. Morehouse, G. Watts, G. Liu, D. N. McMurray, K. P. LeClair, S. A. Porcelli, and M. B. Brenner Immunization with a mycobacterial lipid vaccine improves pulmonary pathology in the guinea pig model of tuberculosis Int. Immunol., August 1, 2003; 15(8): 915 - 925. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Dascher, K. Hiromatsu, X. Xiong, M. Sugita, J. E. Buhlmann, I. L. Dodge, S. Y. Lee, C. Roura-Mir, G. F. Watts, C. J. Roy, et al. Conservation of CD1 Intracellular Trafficking Patterns Between Mammalian Species J. Immunol., December 15, 2002; 169(12): 6951 - 6958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kawahara, T. Nakasone, and M. Honda Dynamics of Gamma Interferon, Interleukin-12 (IL-12), IL-10, and Transforming Growth Factor {beta} mRNA Expression in Primary Mycobacterium bovis BCG Infection in Guinea Pigs Measured by a Real-Time Fluorogenic Reverse Transcription-PCR Assay Infect. Immun., December 1, 2002; 70(12): 6614 - 6620. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Lazarevic and J. Flynn CD8+ T Cells in Tuberculosis Am. J. Respir. Crit. Care Med., October 15, 2002; 166(8): 1116 - 1121. [Full Text] [PDF]
|
|
|
|
|
|
K. Hiromatsu, C. C. Dascher, K. P. LeClair, M. Sugita, S. T. Furlong, M. B. Brenner, and S. A. Porcelli Induction of CD1-Restricted Immune Responses in Guinea Pigs by Immunization with Mycobacterial Lipid Antigens J. Immunol., July 1, 2002; 169(1): 330 - 339. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.M. Rhind CD1--The Pathology Perspective Vet. Pathol., November 1, 2001; 38(6): 611 - 619. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. d. C. Salamone, A. K. Mendiguren, G. V. Salamone, and L. Fainboim Membrane trafficking of CD1c on activated T cells J. Leukoc. Biol., October 1, 2001; 70(4): 567 - 577. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-H. Park, A. Weiss, K. Benlagha, T. Kyin, L. Teyton, and A. Bendelac The Mouse CD1d-restricted Repertoire Is Dominated by a Few Autoreactive T Cell Receptor Families J. Exp. Med., April 9, 2001; 193(8): 893 - 904. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Hayes and K. L. Knight Group 1 CD1 Genes in Rabbit J. Immunol., January 1, 2001; 166(1): 403 - 410. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Angenieux, J. Salamero, D. Fricker, J.-P. Cazenave, B. Goud, D. Hanau, and H. de la Salle Characterization of CD1e, a Third Type of CD1 Molecule Expressed in Dendritic Cells J. Biol. Chem., November 22, 2000; 275(48): 37757 - 37764. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
