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-Chain Dependent Recruitment of Tyrosine Kinases to Membrane Rafts by the Human IgA Receptor Fc
R1
Department of Microbiology, Dartmouth Medical School, Lebanon, NH 03756
We show that the human IgA receptor, Fc
R, redistributes to
plasma membrane rafts after cross-linking and that tyrosine kinases are
relocated to these sites following FcR capping. We demonstrate by
confocal microscopy that FcR caps in membrane rafts by a
-chain-independent mechanism but that -chain expression is
necessary for Lyn redistribution. Immunoblotting of rafts isolated by
sucrose density gradient centrifugation demonstrated recruitment of
-chain and phosphorylated tyrosine kinases Lyn and Bruton’s
tyrosine kinase to membrane rafts after FcR cross-linking.
Time-dependent differences in Lyn phosphorylation and Bruton’s
tyrosine kinase distribution were observed between cells expressing
FcR plus -chain and cells expressing FcR only. This study
defines early FcR-triggered membrane dynamics that take place before
FcR internalization.
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