The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Verdijk, R. M.
Right arrow Articles by Goulmy, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Verdijk, R. M.
Right arrow Articles by Goulmy, E.
The Journal of Immunology, 1999, 163: 57-61.
Copyright © 1999 by The American Association of Immunologists

Polyriboinosinic Polyribocytidylic Acid (Poly(I:C)) Induces Stable Maturation of Functionally Active Human Dendritic Cells1

Rob M. Verdijk2, Tuna Mutis, Ben Esendam, Janine Kamp, Cees J. M. Melief, Anneke Brand and Els Goulmy

Department of Immunohematology and Blood Bank, Leiden University Medical Center, Leiden, The Netherlands

For vaccination strategies and adoptive immunotherapy purposes, immature dendritic cells (DC) can be generated from adherent monocytes using GM-CSF and IL-4. Presently, the only clinically applicable method to induce stable maturation of DC is the use of supernatants of activated monocytes (monocyte-conditioned medium (MCM)). MCM contains an undefined mixture of cytokines and is difficult to standardize. Here we report that stable maturation of DC can be simply induced by the addition of polyriboinosinic polyribocytidylic acid (poly(I:C)), a synthetic dsRNA clinically applied as an immunomodulator. Poly(I:C)-treated DC show a mature phenotype with high expression levels of HLA-DR, CD86, and the DC maturation marker CD83. This mature phenotype is retained for 48 h after cytokine withdrawal. In contrast to untreated DC, poly(I:C)-treated DC down-regulate pinocytosis, produce high levels of IL-12 and low levels of IL-10, induce strong T cell proliferation in a primary allo MLR, and effectively present peptide Ags to HLA class I-restricted CTL. In conclusion, we present a simple methodology for the preparation of clinically applicable mature DC.




This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
X.-L. Huang, Z. Fan, L. Borowski, and C. R. Rinaldo
Maturation of dendritic cells for enhanced activation of anti-HIV-1 CD8+ T cell immunity
J. Leukoc. Biol., June 1, 2008; 83(6): 1530 - 1540.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. M. Lundberg, S. K. Drexler, C. Monaco, L. M. Williams, S. M. Sacre, M. Feldmann, and B. M. Foxwell
Key differences in TLR3/poly I:C signaling and cytokine induction by human primary cells: a phenomenon absent from murine cell systems
Blood, November 1, 2007; 110(9): 3245 - 3252.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. McBride, K. Hoebe, P. Georgel, and E. Janssen
Cell-Associated Double-Stranded RNA Enhances Antitumor Activity through the Production of Type I IFN
J. Immunol., November 1, 2006; 177(9): 6122 - 6128.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K.-M. Lee, J. P. Forman, M. E. McNerney, S. Stepp, S. Kuppireddi, D. Guzior, Y. E. Latchman, M. H. Sayegh, H. Yagita, C.-K. Park, et al.
Requirement of homotypic NK-cell interactions through 2B4(CD244)/CD48 in the generation of NK effector functions
Blood, April 15, 2006; 107(8): 3181 - 3188.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
N. Hirano, M. O. Butler, Z. Xia, S. Ansen, M. S. von Bergwelt-Baildon, D. Neuberg, G. J. Freeman, and L. M. Nadler
Engagement of CD83 ligand induces prolonged expansion of CD8+ T cells and preferential enrichment for antigen specificity
Blood, February 15, 2006; 107(4): 1528 - 1536.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
V. Triantis, D. E. Trancikova, M. W. G. Looman, F. C. Hartgers, R. A. J. Janssen, and G. J. Adema
Identification and Characterization of DC-SCRIPT, a Novel Dendritic Cell-Expressed Member of the Zinc Finger Family of Transcriptional Regulators
J. Immunol., January 15, 2006; 176(2): 1081 - 1089.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
N. Guriec, C. Daniel, K. Le Ster, E. Hardy, and C. Berthou
Cytokine-regulated expression and inhibitory function of Fc{gamma}RIIB1 and -B2 receptors in human dendritic cells
J. Leukoc. Biol., January 1, 2006; 79(1): 59 - 70.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
G. Freer, D. Matteucci, P. Mazzetti, L. Bozzacco, and M. Bendinelli
Generation of Feline Dendritic Cells Derived from Peripheral Blood Monocytes for In Vivo Use
Clin. Vaccine Immunol., October 1, 2005; 12(10): 1202 - 1208.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
K. Breckpot, J. Corthals, A. Bonehill, A. Michiels, S. Tuyaerts, C. Aerts, C. Heirman, and K. Thielemans
Dendritic cells differentiated in the presence of IFN-{beta} and IL-3 are potent inducers of an antigen-specific CD8+ T cell response
J. Leukoc. Biol., October 1, 2005; 78(4): 898 - 908.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Ma and A. C. Ross
The anti-tetanus immune response of neonatal mice is augmented by retinoic acid combined with polyriboinosinic:polyribocytidylic acid
PNAS, September 20, 2005; 102(38): 13556 - 13561.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S.-Y. Dai, R. Nakagawa, A. Itoh, H. Murakami, Y. Kashio, H. Abe, S. Katoh, K. Kontani, M. Kihara, S.-L. Zhang, et al.
Galectin-9 Induces Maturation of Human Monocyte-Derived Dendritic Cells
J. Immunol., September 1, 2005; 175(5): 2974 - 2981.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. Ma, Q. Chen, and A. C. Ross
Retinoic Acid and Polyriboinosinic:Polyribocytidylic Acid Stimulate Robust Anti-Tetanus Antibody Production while Differentially Regulating Type 1/Type 2 Cytokines and Lymphocyte Populations
J. Immunol., June 15, 2005; 174(12): 7961 - 7969.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
E. Barnes, M. Salio, V. Cerundolo, J. Medlin, S. Murphy, G. Dusheiko, and P. Klenerman
Impact of Alpha Interferon and Ribavirin on the Function of Maturing Dendritic Cells
Antimicrob. Agents Chemother., September 1, 2004; 48(9): 3382 - 3389.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Elsen, J. Doussiere, C. L. Villiers, M. Faure, R. Berthier, A. Papaioannou, N. Grandvaux, P. N. Marche, and P. V. Vignais
Cryptic O2--generating NADPH oxidase in dendritic cells
J. Cell Sci., May 1, 2004; 117(11): 2215 - 2226.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
R. Rouas, P. Lewalle, F. El Ouriaghli, B. Nowak, H. Duvillier, and P. Martiat
Poly(I:C) used for human dendritic cell maturation preserves their ability to secondarily secrete bioactive IL-12
Int. Immunol., May 1, 2004; 16(5): 767 - 773.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Wen, J. Peng, Z. Li, and F. S. Wong
The Effect of Innate Immunity on Autoimmune Diabetes and the Expression of Toll-Like Receptors on Pancreatic Islets
J. Immunol., March 1, 2004; 172(5): 3173 - 3180.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
C. Fujimoto, Y. Nakagawa, K. Ohara, and H. Takahashi
Polyriboinosinic polyribocytidylic acid [poly(I:C)]/TLR3 signaling allows class I processing of exogenous protein and induction of HIV-specific CD8+ cytotoxic T lymphocytes
Int. Immunol., January 1, 2004; 16(1): 55 - 63.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
S. Della Bella, S. Nicola, A. Riva, M. Biasin, M. Clerici, and M. L. Villa
Functional repertoire of dendritic cells generated in granulocyte macrophage-colony stimulating factor and interferon-{alpha}
J. Leukoc. Biol., January 1, 2004; 75(1): 106 - 116.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Matsumoto, K. Funami, M. Tanabe, H. Oshiumi, M. Shingai, Y. Seto, A. Yamamoto, and T. Seya
Subcellular Localization of Toll-Like Receptor 3 in Human Dendritic Cells
J. Immunol., September 15, 2003; 171(6): 3154 - 3162.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. K. Means, F. Hayashi, K. D. Smith, A. Aderem, and A. D. Luster
The Toll-Like Receptor 5 Stimulus Bacterial Flagellin Induces Maturation and Chemokine Production in Human Dendritic Cells
J. Immunol., May 15, 2003; 170(10): 5165 - 5175.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. Stober, I. Jomantaite, R. Schirmbeck, and J. Reimann
NKT Cells Provide Help for Dendritic Cell-Dependent Priming of MHC Class I-Restricted CD8+ T Cells In Vivo
J. Immunol., March 1, 2003; 170(5): 2540 - 2548.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
L. Shi, S. H. Fatemi, R. W. Sidwell, and P. H. Patterson
Maternal Influenza Infection Causes Marked Behavioral and Pharmacological Changes in the Offspring
J. Neurosci., January 1, 2003; 23(1): 297 - 302.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
K. Duperrier, A. Farre, J. Bienvenu, N. Bleyzac, J. Bernaud, L. Gebuhrer, D. Rigal, and A. Eljaafari
Cyclosporin A inhibits dendritic cell maturation promoted by TNF-{alpha} or LPS but not by double-stranded RNA or CD40L
J. Leukoc. Biol., November 1, 2002; 72(5): 953 - 961.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. Mommaas, J. Kamp, J.-W. Drijfhout, N. Beekman, F. Ossendorp, P. van Veelen, J. den Haan, E. Goulmy, and T. Mutis
Identification of a Novel HLA-B60-Restricted T Cell Epitope of the Minor Histocompatibility Antigen HA-1 Locus
J. Immunol., September 15, 2002; 169(6): 3131 - 3136.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. Ponsaerts, G. Van den Bosch, N. Cools, A. Van Driessche, G. Nijs, M. Lenjou, F. Lardon, C. Van Broeckhoven, D. R. Van Bockstaele, Z. N. Berneman, et al.
Messenger RNA Electroporation of Human Monocytes, Followed by Rapid In Vitro Differentiation, Leads to Highly Stimulatory Antigen-Loaded Mature Dendritic Cells
J. Immunol., August 15, 2002; 169(4): 1669 - 1675.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. Riedl, D. Stober, C. Oehninger, K. Melber, J. Reimann, and R. Schirmbeck
Priming Th1 Immunity to Viral Core Particles Is Facilitated by Trace Amounts of RNA Bound to Its Arginine-Rich Domain
J. Immunol., May 15, 2002; 168(10): 4951 - 4959.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
T. R. Gibb, D. A. Norwood Jr., N. Woollen, and E. A. Henchal
Viral Replication and Host Gene Expression in Alveolar Macrophages Infected with Ebola Virus (Zaire Strain)
Clin. Vaccine Immunol., January 1, 2002; 9(1): 19 - 27.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. A. Hobbs, S. Cho, T. J. Roberts, V. Sriram, J. Zhang, M. Xu, and R. R. Brutkiewicz
Selective Loss of Natural Killer T Cells by Apoptosis following Infection with Lymphocytic Choriomeningitis Virus
J. Virol., November 15, 2001; 75(22): 10746 - 10754.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Somersan, M. Larsson, J. F. Fonteneau, S. Basu, P. Srivastava, and N. Bhardwaj
Primary Tumor Tissue Lysates Are Enriched in Heat Shock Proteins and Induce the Maturation of Human Dendritic Cells
J. Immunol., November 1, 2001; 167(9): 4844 - 4852.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P.-O. Vidalain, O. Azocar, H. Yagita, C. Rabourdin-Combe, and C. Servet-Delprat
Cytotoxic Activity of Human Dendritic Cells Is Differentially Regulated by Double-Stranded RNA and CD40 Ligand
J. Immunol., October 1, 2001; 167(7): 3765 - 3772.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. R. Bleharski, K. R. Niazi, P. A. Sieling, G. Cheng, and R. L. Modlin
Signaling Lymphocytic Activation Molecule Is Expressed on CD40 Ligand-Activated Dendritic Cells and Directly Augments Production of Inflammatory Cytokines
J. Immunol., September 15, 2001; 167(6): 3174 - 3181.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Th. den Boer, L. Diehl, G. J. D. van Mierlo, E. I. H. van der Voort, M. F. Fransen, P. Krimpenfort, C. J. M. Melief, R. Offringa, and R. E. M. Toes
Longevity of Antigen Presentation and Activation Status of APC Are Decisive Factors in the Balance Between CTL Immunity Versus Tolerance
J. Immunol., September 1, 2001; 167(5): 2522 - 2528.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Bauer, V. Redecke, J. W. Ellwart, B. Scherer, J.-P. Kremer, H. Wagner, and G. B. Lipford
Bacterial CpG-DNA Triggers Activation and Maturation of Human CD11c-, CD123+ Dendritic Cells
J. Immunol., April 15, 2001; 166(8): 5000 - 5007.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. Rea, M. J. E. Havenga, M. van den Assem, R. P. M. Sutmuller, A. Lemckert, R. C. Hoeben, A. Bout, C. J. M. Melief, and R. Offringa
Highly Efficient Transduction of Human Monocyte-Derived Dendritic Cells with Subgroup B Fiber-Modified Adenovirus Vectors Enhances Transgene-Encoded Antigen Presentation to Cytotoxic T Cells
J. Immunol., April 15, 2001; 166(8): 5236 - 5244.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Kadowaki, S. Antonenko, and Y.-J. Liu
Distinct CpG DNA and Polyinosinic-Polycytidylic Acid Double-Stranded RNA, Respectively, Stimulate CD11c- Type 2 Dendritic Cell Precursors and CD11c+ Dendritic Cells to Produce Type I IFN
J. Immunol., February 15, 2001; 166(4): 2291 - 2295.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. Weissman, H. Ni, D. Scales, A. Dude, J. Capodici, K. McGibney, A. Abdool, S. N. Isaacs, G. Cannon, and K. Kariko
HIV Gag mRNA Transfection of Dendritic Cells (DC) Delivers Encoded Antigen to MHC Class I and II Molecules, Causes DC Maturation, and Induces a Potent Human In Vitro Primary Immune Response
J. Immunol., October 15, 2000; 165(8): 4710 - 4717.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. Yang, Q. Chen, S. Stoll, X. Chen, O. M. Z. Howard, and J. J. Oppenheim
Differential Regulation of Responsiveness to fMLP and C5a Upon Dendritic Cell Maturation: Correlation with Receptor Expression
J. Immunol., September 1, 2000; 165(5): 2694 - 2702.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Larsson, D. Messmer, S. Somersan, J.-F. Fonteneau, S. M. Donahoe, M. Lee, P. R. Dunbar, V. Cerundolo, I. Julkunen, D. F. Nixon, et al.
Requirement of Mature Dendritic Cells for Efficient Activation of Influenza A-Specific Memory CD8+ T Cells
J. Immunol., August 1, 2000; 165(3): 1182 - 1190.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
B. Sauter, M. L. Albert, L. Francisco, M. Larsson, S. Somersan, and N. Bhardwaj
Consequences of Cell Death: Exposure to Necrotic Tumor Cells, but Not Primary Tissue Cells or Apoptotic Cells, Induces the Maturation of Immunostimulatory Dendritic Cells
J. Exp. Med., February 7, 2000; 191(3): 423 - 434.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. Brossart, B. Spahlinger, F. Grunebach, G. Stuhler, V. L. Reichardt, L. Kanz, W. Brugger;, T. Mutis, and E. Goulmy
Induction of Minor Histocompatibility Antigen HA-1-Specific Cytotoxic T Cells for the Treatment of Leukemia After Allogeneic Stem Cell Transplantation
Blood, December 15, 1999; 94(12): 4374 - 4376.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.