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Departments of
*
Otolaryngology and
Immunology and Parasitology, Yokohama City University School of Medicine, Yokohama, Japan
This study examined the adhesive interaction of peripheral blood
monocytes with VCAM-1 and analyzed the effect of P-selectin binding to
monocytes on the adhesive interaction with VCAM-1 under flow
conditions. P-selectin glycoprotein ligand-1 is expressed on most
monocytes. Furthermore, most monocytes bind soluble P-selectin derived
from platelets. P-selectin binding to monocytes did not alter the
amount of expression of 
4 integrin on monocytes.
However, the mean channel fluorescence value for binding Cy2-conjugated
soluble VCAM-1 to P-selectin-bound monocytes was slightly more than
that for binding Cy2-conjugated soluble VCAM-1 to untreated monocytes.
Under flow conditions, the number of P-selectin-bound monocytes bound
to VCAM-1 was much higher than that of untreated monocytes bound to
VCAM-1. These bindings were abolished by pretreatment of untreated
monocytes and P-selectin-bound monocytes with anti-VCAM-1 mAb or
anti-4 integrin mAb. Furthermore, P-selectin binding
to monocytes increased shear resistance and thus increased the adhesive
strength of monocytes to VCAM-1. These findings indicate that
P-selectin binding to monocytes enhances the adhesive interaction of
monocytes with VCAM-1. It is suggested that P-selectin glycoprotein
ligand-1/P-selectin interaction and 4 integrin/VCAM-1
interaction can act sequentially in the adhesion cascade that regulates
monocyte trafficking to inflammatory and atherosclerotic
lesion.
This article has been cited by other articles:
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T. Inoue, Y. Tsuzuki, K. Matsuzaki, H. Matsunaga, J. Miyazaki, R. Hokari, Y. Okada, A. Kawaguchi, S. Nagao, K. Itoh, et al. Blockade of PSGL-1 attenuates CD14+ monocytic cell recruitment in intestinal mucosa and ameliorates ileitis in SAMP1/Yit mice J. Leukoc. Biol., March 1, 2005; 77(3): 287 - 295. [Abstract] [Full Text] [PDF]
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 C. K. D. Ng, S. S. Deshpande, K. Irani, and B. R. Alevriadou Adhesion of flowing monocytes to hypoxia-reoxygenation-exposed endothelial cells: role of Rac1, ROS, and VCAM-1 Am J Physiol Cell Physiol, July 1, 2002; 283(1): C93 - C102. [Abstract] [Full Text] [PDF]
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A. Stucki, A.-S. Rivier, M. Gikic, N. Monai, M. Schapira, and O. Spertini Endothelial cell activation by myeloblasts: molecular mechanisms of leukostasis and leukemic cell dissemination Blood, April 1, 2001; 97(7): 2121 - 2129. [Abstract] [Full Text] [PDF]
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