| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
-Induced Production of IL-6 and IL-8 from Cultured Synovial Fibroblasts1
Departments of
*
Molecular Genetics and
Orthopedics, Nagoya City University Medical School, Nagoya, Japan; and
Louis Pasteur Center for Medical Research, Kyoto, Japan
Thioredoxin (TRX) is a cellular reducing catalyst induced by
oxidative stress and is involved in the redox regulation of
transcription factors such as NF-
B. We found that the serum TRX
concentration was elevated in patients with rheumatoid arthritis (RA)
as compared with values from healthy individuals and patients with
osteoarthritis (33.6 ± 35.1 vs 11.8 ± 6.6 ng/ml,
p < 0.01). Moreover, the TRX concentration in the
synovial fluid (SF) was much more elevated in RA patients than in
osteoarthritis patients (103.4 ± 53.3 vs 24.6 ± 17.4 ng/ml,
p < 0.001). Multiple regression analysis revealed
that the serum C-reactive protein value was better correlated with the
linear combination of SF TNF-
and SF TRX values than with SF TNF-
alone, suggesting that TRX might play a subsidiary role in the
rheumatoid inflammation. We thus examined the effect of TRX on the
TNF--induced IL-6 and IL-8 production using rheumatoid synovial
fibroblast cultures. The extents of IL-6 and IL-8 production in
response to TNF- were greatly augmented by TRX as compared with
TNF- alone. TRX alone did not have such effects. We also found that
TRX appeared to accelerate the nuclear translocation of NF-B, a
major transcriptional regulator for production of IL-6 and IL-8 on
stimulation with TNF-. Consistent with these findings, the IB
phosphorylation at Ser32 and its subsequent degradation in
response to TNF- was facilitated by TRX. These findings indicate
that the elevated TRX concentration in SF of RA patients might be
involved in the aggravation of rheumatoid inflammation by augmenting
the NF-B activation pathway.
This article has been cited by other articles:
|
|
 |
|
  B. Henderson, E. Allan, and A. R. M. Coates Stress Wars: the Direct Role of Host and Bacterial Molecular Chaperones in Bacterial Infection Infect. Immun., July 1, 2006; 74(7): 3693 - 3706. [Full Text] [PDF]
|
|
|
|
 |
|
 L. Billiet, C. Furman, G. Larigauderie, C. Copin, K. Brand, J.-C. Fruchart, and M. Rouis Extracellular Human Thioredoxin-1 Inhibits Lipopolysaccharide-induced Interleukin-1{beta} Expression in Human Monocyte-derived Macrophages J. Biol. Chem., December 2, 2005; 280(48): 40310 - 40318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Pekkari, M. T. Goodarzi, A. Scheynius, A. Holmgren, and J. Avila-Carino Truncated thioredoxin (Trx80) induces differentiation of human CD14+ monocytes into a novel cell type (TAMs) via activation of the MAP kinases p38, ERK, and JNK Blood, February 15, 2005; 105(4): 1598 - 1605. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Porubsky, H. Schmid, M. Bonrouhi, M. Kretzler, E. Malle, P. J. Nelson, and H.-J. Grone Influence of Native and Hypochlorite-Modified Low-Density Lipoprotein on Gene Expression in Human Proximal Tubular Epithelium Am. J. Pathol., June 1, 2004; 164(6): 2175 - 2187. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Oslund, L. A. Miller, J. L. Usachenko, N. K. Tyler, R. Wu, and D. M. Hyde Oxidant-Injured Airway Epithelial Cells Upregulate Thioredoxin but Do Not Produce Interleukin-8 Am. J. Respir. Cell Mol. Biol., May 1, 2004; 30(5): 597 - 604. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Felix, S. Gerstmeier, A. Kyriakopoulos, O. M. Z. Howard, H.-F. Dong, M. Eckhaus, D. Behne, G. W. Bornkamm, and S. Janz Selenium Deficiency Abrogates Inflammation-Dependent Plasma Cell Tumors in Mice Cancer Res., April 15, 2004; 64(8): 2910 - 2917. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yamawaki, J. Haendeler, and B. C. Berk Thioredoxin: A Key Regulator of Cardiovascular Homeostasis Circ. Res., November 28, 2003; 93(11): 1029 - 1033. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kunchithapautham, B. Padmavathi, R. B. Narayanan, P. Kaliraj, and A. L. Scott Thioredoxin from Brugia malayi: Defining a 16-Kilodalton Class of Thioredoxins from Nematodes Infect. Immun., July 1, 2003; 71(7): 4119 - 4126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Liu and W. Min Thioredoxin Promotes ASK1 Ubiquitination and Degradation to Inhibit ASK1-Mediated Apoptosis in a Redox Activity-Independent Manner Circ. Res., June 28, 2002; 90(12): 1259 - 1266. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Companjen, L. I. v. d. Wel, L. Boon, E. P. Prens, and J. D. Laman CD40 ligation-induced cytokine production in human skin explants is partly mediated via IL-1 Int. Immunol., June 1, 2002; 14(6): 669 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S Drinda, S Franke, C C Canet, P Petrow, R Brauer, C Huttich, G Stein, and G Hein Identification of the advanced glycation end products N{varepsilon}-carboxymethyllysine in the synovial tissue of patients with rheumatoid arthritis Ann Rheum Dis, June 1, 2002; 61(6): 488 - 492. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. I. Gringhuis, E. A. M. Papendrecht-van der Voort, A. Leow, E. W. N. Levarht, F. C. Breedveld, and C. L. Verweij Effect of Redox Balance Alterations on Cellular Localization of LAT and Downstream T-Cell Receptor Signaling Pathways Mol. Cell. Biol., January 15, 2002; 22(2): 400 - 411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Pekkari, J. Avila-Carino, A. Bengtsson, R. Gurunath, A. Scheynius, and A. Holmgren Truncated thioredoxin (Trx80) induces production of interleukin-12 and enhances CD14 expression in human monocytes Blood, May 15, 2001; 97(10): 3184 - 3190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Henderson, P. Tabona, S. Poole, and S. P. Nair Cloning and Expression of the Actinobacillus actinomycetemcomitans Thioredoxin (trx) Gene and Assessment of Cytokine Inhibitory Activity Infect. Immun., January 1, 2001; 69(1): 154 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Georganas, H. Liu, H. Perlman, A. Hoffmann, B. Thimmapaya, and R. M. Pope Regulation of IL-6 and IL-8 Expression in Rheumatoid Arthritis Synovial Fibroblasts: the Dominant Role for NF-{kappa}B But Not C/EBP{beta} or c-Jun J. Immunol., December 15, 2000; 165(12): 7199 - 7206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Schoonbroodt, V. Ferreira, M. Best-Belpomme, J. R. Boelaert, S. Legrand-Poels, M. Korner, and J. Piette Crucial Role of the Amino-Terminal Tyrosine Residue 42 and the Carboxyl-Terminal PEST Domain of I{kappa}B{alpha} in NF-{kappa}B Activation by an Oxidative Stress J. Immunol., April 15, 2000; 164(8): 4292 - 4300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. I. Gringhuis, A. Leow, E. A. M. Papendrecht-van der Voort, P. H. J. Remans, F. C. Breedveld, and C. L. Verweij Displacement of Linker for Activation of T Cells from the Plasma Membrane Due to Redox Balance Alterations Results in Hyporesponsiveness of Synovial Fluid T Lymphocytes in Rheumatoid Arthritis J. Immunol., February 15, 2000; 164(4): 2170 - 2179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tetsuka, H. Uranishi, H. Imai, T. Ono, S.-i. Sonta, N. Takahashi, K. Asamitsu, and T. Okamoto Inhibition of Nuclear Factor-kappa B-mediated Transcription by Association with the Amino-terminal Enhancer of Split, a Groucho-related Protein Lacking WD40 Repeats J. Biol. Chem., February 11, 2000; 275(6): 4383 - 4390. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
