HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hauser, C. Articles by Hügin, A. W. Search for Related Content PubMed PubMed Citation Articles by Hauser, C. Articles by Hügin, A. W. Pubmed/NCBI databases Substance via MeSH

Protective Immunity from Naive CD8⁺ T Cells Activated In Vitro with MHC Class I Binding Immunogenic Peptides and IL-2 in the Absence of Specialized APCs¹

Conrad Hauser^1,*,, Frank Zipprich^3,*, Isabelle Leblond^*, Susanne Wirth^* and Ambros W. Hügin

^* Allergy Unit, Division of Immunology and Allergy, and Department of Dermatology, Hôpital Cantonal Universitaire, Geneva, Switzerland

Ag-specific CTL can protect against tumors and some viral infections and may be useful for adoptive immunotherapy. Here, we show that purified CD8⁺ T cells from naive C57BL/6 mice can be primed in vitro with different immunogenic peptides, which bind to MHC class I gene products, and IL-2 to exhibit specific and MHC-restricted effector function in vitro and in vivo protection against lymphocytic choriomeningitis virus infection and B16.F10 melanoma lung metastases. Limiting dilution assays in the absence of feeder cells with highly purified CD8⁺ T cells from two transgenic mice strains, each expressing a different MHC class I-restricted TCR, indicated that only peptide and IL-2, but not TCR^- cells, were required for the growth of naive CD8⁺ T cells. These alternative minimal requirements for the activation and expansion of specific CD8⁺ T lymphocytes, without the need for professional APC, may be exploited for adoptive immunotherapy.

This article has been cited by other articles:

				A. Kelso, E. O. Costelloe, B. J. Johnson, P. Groves, K. Buttigieg, and D. R. Fitzpatrick The genes for perforin, granzymes A-C and IFN-{gamma} are differentially expressed in single CD8+ T cells during primary activation Int. Immunol., June 1, 2002; 14(6): 605 - 613. [Abstract] [Full Text] [PDF]

				J.-I. Sin, J. Kim, C. Patchuk, and D. B. Weiner Interleukin 7 Can Enhance Antigen-Specific Cytotoxic-T-Lymphocyte and/or Th2-Type Immune Responses In Vivo Clin. Vaccine Immunol., September 1, 2000; 7(5): 751 - 758. [Abstract] [Full Text] [PDF]