The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kubota, A.
Right arrow Articles by Takei, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kubota, A.
Right arrow Articles by Takei, F.
The Journal of Immunology, 1999, 163: 212-216.
Copyright © 1999 by The American Association of Immunologists

Diversity of NK Cell Receptor Repertoire in Adult and Neonatal Mice1

Akira Kubota2,*, Satoko Kubota2,*, Stefan Lohwasser*, Dixie L. Mager*,{dagger} and Fumio Takei3,*,{ddagger}

* Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, Canada; and Departments of {dagger} Medical Genetics and {ddagger} Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada

Murine NK cytotoxicity is regulated by two families of MHC class I-specific receptors, namely Ly49 and CD94/NKG2. We developed a single-cell RT-PCR method to analyze expression of all known Ly49 and NKG2A genes in individual NK cells and determined the receptor repertoires of NK cells from adult and neonatal (1-wk-old) C57BL/6 mice. In adult mouse NK cells, up to six different receptors were coexpressed in random combinations. Of 62 NK cells examined, 42 different patterns of receptor expression were observed. Most of them expressed at least one Ly49, whereas NKG2A was detected in 32% of the cells. Over 75% of them expressed Ly49C, I, or NKG2A, which are thought to recognize self-class I MHC (H-2b). Coexpression of multiple Ly49 receptors and NKG2A was stochastic. In contrast, very few neonatal NK cells expressed any Ly49, but almost 60% of them expressed NKG2A. These results demonstrate that adult NK cells are quite heterogeneous and have diverse receptor repertoires. They also suggest that the expression of NKG2A precedes Ly49 expression in NK cell ontogeny, and NKG2A is a major inhibitory receptor in neonatal NK cells.




This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
A. Rouhi, C. B. Lai, T. P. Cheng, F. Takei, W. M. Yokoyama, and D. L. Mager
Evidence for high bi-allelic expression of activating Ly49 receptors
Nucleic Acids Res., September 1, 2009; 37(16): 5331 - 5342.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. G. Aust, F. Gays, K. M. Mickiewicz, E. Buchanan, and C. G. Brooks
The Expression and Function of the NKRP1 Receptor Family in C57BL/6 Mice
J. Immunol., July 1, 2009; 183(1): 106 - 116.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. Brodin, T. Lakshmikanth, S. Johansson, K. Karre, and P. Hoglund
The strength of inhibitory input during education quantitatively tunes the functional responsiveness of individual natural killer cells
Blood, March 12, 2009; 113(11): 2434 - 2441.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
E. T. Durkin, K. A. Jones, D. Rajesh, and A. F. Shaaban
Early chimerism threshold predicts sustained engraftment and NK-cell tolerance in prenatal allogeneic chimeras
Blood, December 15, 2008; 112(13): 5245 - 5253.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Yawata, N. Yawata, M. Draghi, F. Partheniou, A.-M. Little, and P. Parham
MHC class I-specific inhibitory receptors and their ligands structure diverse human NK-cell repertoires toward a balance of missing self-response
Blood, September 15, 2008; 112(6): 2369 - 2380.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
A. Iannello, O. Debbeche, S. Samarani, and A. Ahmad
Antiviral NK cell responses in HIV infection: I. NK cell receptor genes as determinants of HIV resistance and progression to AIDS
J. Leukoc. Biol., July 1, 2008; 84(1): 1 - 26.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. W. MacFarlane IV, T. Yamazaki, M. Fang, L. J. Sigal, T. Kurosaki, and K. S. Campbell
Enhanced NK-cell development and function in BCAP-deficient mice
Blood, July 1, 2008; 112(1): 131 - 140.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
Y. Hayakawa, S. V. Watt, K. Takeda, and M. J. Smyth
Distinct receptor repertoire formation in mouse NK cell subsets regulated by MHC class I expression
J. Leukoc. Biol., January 1, 2008; 83(1): 106 - 111.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
V. Pascal, N. R. Nathan, E. Claudio, U. Siebenlist, and S. K. Anderson
NF-{kappa}B p50/p65 Affects the Frequency of Ly49 Gene Expression by NK Cells
J. Immunol., August 1, 2007; 179(3): 1751 - 1759.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Scarpellino, F. Oeschger, P. Guillaume, J. D. Coudert, F. Levy, G. Leclercq, and W. Held
Interactions of Ly49 Family Receptors with MHC Class I Ligands in trans and cis
J. Immunol., February 1, 2007; 178(3): 1277 - 1284.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
S. Iizuka, T. Kaifu, A. Nakamura, M. Obinata, and T. Takai
Establishment and Functional Characterization of Novel Natural Killer Cell Lines Derived from a Temperature-Sensitive SV40 Large T Antigen Transgenic Mouse
J. Biochem., August 1, 2006; 140(2): 255 - 265.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. L. Rogers, A. Rouhi, F. Takei, and D. L. Mager
A Role for DNA Hypomethylation and Histone Acetylation in Maintaining Allele-Specific Expression of Mouse NKG2A in Developing and Mature NK Cells
J. Immunol., July 1, 2006; 177(1): 414 - 421.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Kveberg, C. J. Back, K.-Z. Dai, M. Inngjerdingen, B. Rolstad, J. C. Ryan, J. T. Vaage, and C. Naper
The Novel Inhibitory NKR-P1C Receptor and Ly49s3 Identify Two Complementary, Functionally Distinct NK Cell Subsets in Rats
J. Immunol., April 1, 2006; 176(7): 4133 - 4140.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Rouhi, L. Gagnier, F. Takei, and D. L. Mager
Evidence for epigenetic maintenance of Ly49A monoallelic gene expression.
J. Immunol., March 1, 2006; 176(5): 2991 - 2999.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. Gays, K. Martin, R. Kenefeck, J. G. Aust, and C. G. Brooks
Multiple Cytokines Regulate the NK Gene Complex-Encoded Receptor Repertoire of Mature NK Cells and T Cells
J. Immunol., September 1, 2005; 175(5): 2938 - 2947.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
N. C. Fernandez, E. Treiner, R. E. Vance, A. M. Jamieson, S. Lemieux, and D. H. Raulet
A subset of natural killer cells achieves self-tolerance without expressing inhibitory receptors specific for self-MHC molecules
Blood, June 1, 2005; 105(11): 4416 - 4423.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. L. DeHart, M. J. Heikens, and S. Tsai
Jagged2 promotes the development of natural killer cells and the establishment of functional natural killer cell lines
Blood, May 1, 2005; 105(9): 3521 - 3527.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
S. Johansson, M. Johansson, E. Rosmaraki, G. Vahlne, R. Mehr, M. Salmon-Divon, F. Lemonnier, K. Karre, and P. Hoglund
Natural killer cell education in mice with single or multiple major histocompatibility complex class I molecules
J. Exp. Med., April 4, 2005; 201(7): 1145 - 1155.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. Regunathan, Y. Chen, D. Wang, and S. Malarkannan
NKG2D receptor-mediated NK cell function is regulated by inhibitory Ly49 receptors
Blood, January 1, 2005; 105(1): 233 - 240.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
A. Ahmad and F. Alvarez
Role of NK and NKT cells in the immunopathogenesis of HCV-induced hepatitis
J. Leukoc. Biol., October 1, 2004; 76(4): 743 - 759.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. Y. Setiady, P. Pramoonjago, and K. S. K. Tung
Requirements of NK Cells and Proinflammatory Cytokines in T Cell-Dependent Neonatal Autoimmune Ovarian Disease Triggered by Immune Complex
J. Immunol., July 15, 2004; 173(2): 1051 - 1058.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. P. Makrigiannis, E. Rousselle, and S. K. Anderson
Independent Control of Ly49g Alleles: Implications for NK Cell Repertoire Selection and Tumor Cell Killing
J. Immunol., February 1, 2004; 172(3): 1414 - 1425.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. M. Tanamachi, D. C. Moniot, D. Cado, S. D. Liu, J. K. Hsia, and D. H. Raulet
Genomic Ly49A Transgenes: Basis of Variegated Ly49A Gene Expression and Identification of a Critical Regulatory Element
J. Immunol., January 15, 2004; 172(2): 1074 - 1082.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Kawamura, R. Koka, A. Ma, and V. Kumar
Differential Roles for IL-15R {alpha}-Chain in NK Cell Development and Ly-49 Induction
J. Immunol., November 15, 2003; 171(10): 5085 - 5090.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. T. Wilhelm, J.-R. Landry, F. Takei, and D. L. Mager
Transcriptional Control of Murine CD94 Gene: Differential Usage of Dual Promoters by Lymphoid Cell Types
J. Immunol., October 15, 2003; 171(8): 4219 - 4226.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
C.-W. Lin, Y.-H. Chen, Y.-C. Chuang, T.-Y. Liu, and S.-M. Hsu
CD94 transcripts imply a better prognosis in nasal-type extranodal NK/T-cell lymphoma
Blood, October 1, 2003; 102(7): 2623 - 2631.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
F. Stevenaert, K. Van Beneden, A. De Creus, V. Debacker, J. Plum, and G. Leclercq
Ly49E expression points toward overlapping, but distinct, natural killer (NK) cell differentiation kinetics and potential of fetal versus adult lymphoid progenitors
J. Leukoc. Biol., June 1, 2003; 73(6): 731 - 738.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
S.-H. Lee, A. Zafer, Y. de Repentigny, R. Kothary, M. L. Tremblay, P. Gros, P. Duplay, J. R. Webb, and S. M. Vidal
Transgenic Expression of the Activating Natural Killer Receptor Ly49H Confers Resistance to Cytomegalovirus in Genetically Susceptible Mice
J. Exp. Med., February 17, 2003; 197(4): 515 - 526.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. H. Meyers, A. Ryu, L. Monney, K. Nguyen, E. A. Greenfield, G. J. Freeman, and V. K. Kuchroo
Cutting Edge: CD94/NKG2 Is Expressed on Th1 But Not Th2 Cells and Costimulates Th1 Effector Functions
J. Immunol., November 15, 2002; 169(10): 5382 - 5386.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. Kouro, V. Kumar, and P. W. Kincade
Relationships between early B- and NK-lineage lymphocyte precursors in bone marrow
Blood, November 15, 2002; 100(10): 3672 - 3680.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. H. Lian, M. Maeda, S. Lohwasser, M. Delcommenne, T. Nakano, R. E. Vance, D. H. Raulet, and F. Takei
Orderly and Nonstochastic Acquisition of CD94/NKG2 Receptors by Developing NK Cells Derived from Embryonic Stem Cells In Vitro
J. Immunol., May 15, 2002; 168(10): 4980 - 4987.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Saleh, A. P. Makrigiannis, D. L. Hodge, and S. K. Anderson
Identification of a Novel Ly49 Promoter That Is Active in Bone Marrow and Fetal Thymus
J. Immunol., May 15, 2002; 168(10): 5163 - 5169.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. Y. Chiang, M. Henson, and I. Stroynowski
The Nonclassical Major Histocompatibility Complex Molecule Qa-2 Protects Tumor Cells from NK Cell- and Lymphokine-Activated Killer Cell-Mediated Cytolysis
J. Immunol., March 1, 2002; 168(5): 2200 - 2211.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
V. Ioannidis, J. Zimmer, F. Beermann, and W. Held
Cre Recombinase-Mediated Inactivation of H-2Dd Transgene Expression: Evidence for Partial Missing Self-Recognition by Ly49A NK Cells
J. Immunol., December 1, 2001; 167(11): 6256 - 6262.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Maeda, S. Lohwasser, T. Yamamura, and F. Takei
Regulation of NKT Cells by Ly49: Analysis of Primary NKT Cells and Generation of NKT Cell Line
J. Immunol., October 15, 2001; 167(8): 4180 - 4186.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Fahlen, U. Lendahl, and C. L. Sentman
MHC Class I-Ly49 Interactions Shape the Ly49 Repertoire on Murine NK Cells
J. Immunol., June 1, 2001; 166(11): 6585 - 6592.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. Kunz and W. Held
Positive and Negative Roles of the Trans-Acting T Cell Factor-1 for the Acquisition of Distinct Ly-49 MHC Class I Receptors by NK Cells
J. Immunol., May 15, 2001; 166(10): 6181 - 6187.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Van Beneden, F. Stevenaert, A. De Creus, V. Debacker, J. De Boever, J. Plum, and G. Leclercq
Expression of Ly49E and CD94/NKG2 on Fetal and Adult NK Cells
J. Immunol., April 1, 2001; 166(7): 4302 - 4311.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
S. Lohwasser, A. Kubota, M. Salcedo, R. H. Lian, and F. Takei1
The non-classical MHC class I molecule Qa-1b inhibits classical MHC class I-restricted cytotoxicity of cytotoxic T lymphocytes
Int. Immunol., March 1, 2001; 13(3): 321 - 327.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
D. M. Tanamachi, T. Hanke, H. Takizawa, A. M. Jamieson, and D. H. Raulet
Expression of Natural Killer Receptor Alleles at Different Ly49 Loci Occurs Independently and Is Regulated by Major Histocompatibility Complex Class I Molecules
J. Exp. Med., February 5, 2001; 193(3): 307 - 316.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. T. Silver, D.-E. Gong, C. S. Chang, A. Amrani, P. Santamaria, and K. P. Kane
Ly-49P Activates NK-Mediated Lysis by Recognizing H-2Dd 1
J. Immunol., August 15, 2000; 165(4): 1771 - 1781.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. Lowin-Kropf and W. Held
Positive Impact of Inhibitory Ly49 Receptor-MHC Class I Interaction on NK Cell Development
J. Immunol., July 1, 2000; 165(1): 91 - 95.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
H. R.C. Smith, H. H. Chuang, L. L. Wang, M. Salcedo, J. W. Heusel, and W. M. Yokoyama
Nonstochastic Coexpression of Activation Receptors on Murine Natural Killer Cells
J. Exp. Med., April 17, 2000; 191(8): 1341 - 1354.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. E. Vance, A. M. Jamieson, D. Cado, and D. H. Raulet
Implications of CD94 deficiency and monoallelic NKG2A expression for natural killer cell development and repertoire formation
PNAS, January 22, 2002; 99(2): 868 - 873.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.