The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nath, N.
Right arrow Articles by Chellappan, S. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nath, N.
Right arrow Articles by Chellappan, S. P.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
The Journal of Immunology, 1999, 162: 5351-5358.
Copyright © 1999 by The American Association of Immunologists

HLA Class I-Mediated Induction of Cell Proliferation Involves Cyclin E-Mediated Inactivation of Rb Function and Induction of E2F Activity1

Niharika Nath, Hong Bian, Elaine F. Reed2 and Srikumar P. Chellappan2,3

Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, NY 10032

Chronic rejection of transplanted organs is manifested as atherosclerosis of the blood vessels of the allograft. HLA class I Ags have been implicated to play a major role in this process, since signaling via HLA class I molecules can induce the proliferation of aortic endothelial as well as smooth muscle cells. In this study, we show that HLA class I-mediated induction of cell proliferation correlates with inactivation of the Rb protein in the T cell line Jurkat as well as human aortic endothelial cells. HLA class I-mediated inactivation of Rb can be inhibited specifically by neutralizing Abs to basic fibroblast growth factor (bFGF), suggesting a role for FGF receptors in the signaling process. Signaling through HLA class I molecules induced cyclin E-associated kinase activity within 4 h in quiescent endothelial cells, and appeared to mediate the inactivation of Rb. A cdk2 inhibitor, Olomoucine, as well as a dominant-negative cdk2 construct prevented HLA class I-mediated inactivation of Rb; in contrast, dominant-negative cdk4 and cdk6 constructs had no effect. Furthermore, there was no increase in cyclin D-associated kinase activity upon HLA class I ligation, suggesting that cyclin E-dependent kinase activity mediates Rb inactivation, leading to E2F activation and cell proliferation.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
Y.-P. Jin, Y. Korin, X. Zhang, P. T. Jindra, E. Rozengurt, and E. F. Reed
RNA Interference Elucidates the Role of Focal Adhesion Kinase in HLA Class I-Mediated Focal Adhesion Complex Formation and Proliferation in Human Endothelial Cells
J. Immunol., June 15, 2007; 178(12): 7911 - 7922.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
V. Solodushko and B. Fouty
Proproliferative phenotype of pulmonary microvascular endothelial cells
Am J Physiol Lung Cell Mol Physiol, March 1, 2007; 292(3): L671 - L677.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
P. Dasgupta, J. Sun, S. Wang, G. Fusaro, V. Betts, J. Padmanabhan, S. M. Sebti, and S. P. Chellappan
Disruption of the Rb-Raf-1 Interaction Inhibits Tumor Growth and Angiogenesis
Mol. Cell. Biol., November 1, 2004; 24(21): 9527 - 9541.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Dasgupta, V. Betts, S. Rastogi, B. Joshi, M. Morris, B. Brennan, D. Ordonez-Ercan, and S. Chellappan
Direct Binding of Apoptosis Signal-regulating Kinase 1 to Retinoblastoma Protein: NOVEL LINKS BETWEEN APOPTOTIC SIGNALING AND CELL CYCLE MACHINERY
J. Biol. Chem., September 10, 2004; 279(37): 38762 - 38769.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
S. Coupel, F. Leboeuf, G. Boulday, J.-P. Soulillou, and B. Charreau
RhoA Activation Mediates Phosphatidylinositol 3-Kinase-Dependent Proliferation of Human Vascular Endothelial Cells: An Alloimmune Mechanism of Chronic Allograft Nephropathy
J. Am. Soc. Nephrol., September 1, 2004; 15(9): 2429 - 2439.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y.-P. Jin, R. P. Singh, Z.-Y. Du, A. K. Rajasekaran, E. Rozengurt, and E. F. Reed
Ligation of HLA Class I Molecules on Endothelial Cells Induces Phosphorylation of Src, Paxillin, and Focal Adhesion Kinase in an Actin-Dependent Manner
J. Immunol., June 1, 2002; 168(11): 5415 - 5423.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. A. Ezhevsky, A. Ho, M. Becker-Hapak, P. K. Davis, and S. F. Dowdy
Differential Regulation of Retinoblastoma Tumor Suppressor Protein by G1 Cyclin-Dependent Kinase Complexes In Vivo
Mol. Cell. Biol., July 15, 2001; 21(14): 4773 - 4784.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. P. Colgan, R. M. Hershberg, G. T. Furuta, and R. S. Blumberg
Ligation of intestinal epithelial CD1d induces bioactive IL-10: Critical role of the cytoplasmic tail in autocrine signaling
PNAS, November 23, 1999; 96(24): 13938 - 13943.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Bian and E. F. Reed
Alloantibody-Mediated Class I Signal Transduction in Endothelial Cells and Smooth Muscle Cells: Enhancement by IFN-{gamma} and TNF-{alpha}
J. Immunol., July 15, 1999; 163(2): 1010 - 1018.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.