| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
*
Cell Biology, Neurobiology, and Anatomy and
Microbiology and Immunology, Loyola University Medical Center, Maywood, IL 60153
The mechanism by which the Th2 cell-dependent Ab response is
modulated by the sympathetic neurotransmitter norepinephrine (NE) was
investigated. Our model system used the severe combined immunodeficient
(scid) mouse that was depleted of NE with
6-hydroxydopamine before reconstitution with a clone of
ß2-adrenergic receptor (ß2AR)neg
KLH-specific Th2 cells and resting trinitrophenyl (TNP)-specific
ß2ARpos B cells enriched from the spleens of unimmunized
mice. Following challenge with TNP-keyhole limpet hemocyanin (KLH), Ab
production in these mice was hapten-, carrier-, and allotype-specific
as well as MHC restricted. Depletion of NE resulted in a 50–75%
suppression of the primary anti-TNP IgM response compared with that
of NE-intact controls, while the secondary IgM response returned to
control levels. In contrast, both the primary and secondary
anti-TNP IgG1 responses were suppressed by 85 and 40%,
respectively. Using NE-intact mice exposed to either a ßAR- or
AR-selective antagonist, the effect of NE on the Ab response was
shown to be mediated by the ßAR. In addition, administration of a
ß2AR-selective agonist to NE-depleted mice partially reversed the
suppressed Ab response that resulted from NE depletion. Expression of
the ß2AR on TNP-specific B cells was confirmed by radioligand
binding, immunofluorescence, and cAMP analysis. Also, while splenic
histology was comparable in NE-intact and NE-depleted mice before Ag
exposure, follicle expansion and germinal center formation were
suppressed in NE-depleted mice after Ag exposure. Taken together, these
results suggest that NE stimulation of the ß2AR expressed on B cells
is necessary for the maintenance of an optimal primary and secondary
Th2 cell-dependent Ab response in vivo.
This article has been cited by other articles:
|
|
 |
|
  E. K. Sloan, J. P. Capitanio, R. P. Tarara, S. P. Mendoza, W. A. Mason, and S. W. Cole Social Stress Enhances Sympathetic Innervation of Primate Lymph Nodes: Mechanisms and Implications for Viral Pathogenesis J. Neurosci., August 15, 2007; 27(33): 8857 - 8865. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Pongratz, J. W. McAlees, D. H. Conrad, R. S. Erbe, K. M. Haas, and V. M. Sanders The Level of IgE Produced by a B Cell Is Regulated by Norepinephrine in a p38 MAPK- and CD23-Dependent Manner. J. Immunol., September 1, 2006; 177(5): 2926 - 2938. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. K. Ganta, B. G. Helwig, F. Blecha, R. R. Ganta, R. Cober, S. Parimi, T. I. Musch, R. J. Fels, and M. J. Kenney Hypothermia-enhanced splenic cytokine gene expression is independent of the sympathetic nervous system Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2006; 291(3): R558 - R565. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. W. Kin and V. M. Sanders It takes nerve to tell T and B cells what to do J. Leukoc. Biol., June 1, 2006; 79(6): 1093 - 1104. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. de Salamanca, K. F. Siemasko, Y. Diebold, M. Calonge, J. Gao, M. Juarez-Campo, and M. E. Stern Expression of Muscarinic and Adrenergic Receptors in Normal Human Conjunctival Epithelium Invest. Ophthalmol. Vis. Sci., February 1, 2005; 46(2): 504 - 513. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Deng, K. Muthu, R. Gamelli, R. Shankar, and S. B. Jones Adrenergic modulation of splenic macrophage cytokine release in polymicrobial sepsis Am J Physiol Cell Physiol, September 1, 2004; 287(3): C730 - C736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Podojil, N. W. Kin, and V. M. Sanders CD86 and {beta}2-Adrenergic Receptor Signaling Pathways, Respectively, Increase Oct-2 and OCA-B Expression and Binding to the 3'-IgH Enhancer in B Cells J. Biol. Chem., May 28, 2004; 279(22): 23394 - 23404. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Kohut, J. R. Thompson, W. Lee, and J. E. Cunnick Exercise training-induced adaptations of immune response are mediated by {beta}-adrenergic receptors in aged but not young mice J Appl Physiol, April 1, 2004; 96(4): 1312 - 1322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Saint-Mezard, C. Chavagnac, S. Bosset, M. Ionescu, E. Peyron, D. Kaiserlian, J.-F. Nicolas, and F. Berard Psychological Stress Exerts an Adjuvant Effect on Skin Dendritic Cell Functions In Vivo J. Immunol., October 15, 2003; 171(8): 4073 - 4080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Podojil and V. M. Sanders Selective Regulation of Mature IgG1 Transcription by CD86 and {beta}2-Adrenergic Receptor Stimulation J. Immunol., May 15, 2003; 170(10): 5143 - 5151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-M. Hogerkorp, S. Bilke, T. Breslin, S. Ingvarsson, and C. A. K. Borrebaeck CD44-stimulated human B cells express transcripts specifically involved in immunomodulation and inflammation as analyzed by DNA microarrays Blood, March 15, 2003; 101(6): 2307 - 2313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Mace, M. Jaume, C. Blanpied, L. Stephan, J. D. Coudert, P. Druet, and G. Dietrich Anti-{micro}-opioid-receptor IgG antibodies are commonly present in serum from healthy blood donors: evidence for a role in apoptotic immune cell death Blood, October 16, 2002; 100(9): 3261 - 3268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Kohm, A. Mozaffarian, and V. M. Sanders B Cell Receptor- and {beta}2-Adrenergic Receptor-Induced Regulation of B7-2 (CD86) Expression in B Cells J. Immunol., June 15, 2002; 168(12): 6314 - 6322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Friedman and D. A. Lawrence Environmental Stress Mediates Changes in Neuroimmunological Interactions Toxicol. Sci., May 1, 2002; 67(1): 4 - 10. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Miura, T. Kudo, A. Matsuki, K. Sekikawa, Y.-I. Tagawa, Y. Iwakura, and A. Nakane Effect of 6-Hydroxydopamine on Host Resistance against Listeria monocytogenes Infection Infect. Immun., December 1, 2001; 69(12): 7234 - 7241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. P. Kohm and V. M. Sanders Norepinephrine and beta 2-Adrenergic Receptor Stimulation Regulate CD4+ T and B Lymphocyte Function in Vitro and in Vivo Pharmacol. Rev., December 1, 2001; 53(4): 487 - 525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Moraska and M. Fleshner Voluntary physical activity prevents stress-induced behavioral depression and anti-KLH antibody suppression Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2001; 281(2): R484 - R489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Kasprowicz, A. P. Kohm, M. T. Berton, A. J. Chruscinski, A. Sharpe, and V. M. Sanders Stimulation of the B Cell Receptor, CD86 (B7-2), and the {beta}2-Adrenergic Receptor Intrinsically Modulates the Level of IgG1 and IgE Produced per B Cell J. Immunol., July 15, 2000; 165(2): 680 - 690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Kohm, Y. Tang2, V. M. Sanders, and S. B. Jones3 Activation of Antigen-Specific CD4+ Th2 Cells and B Cells In Vivo Increases Norepinephrine Release in the Spleen and Bone Marrow J. Immunol., July 15, 2000; 165(2): 725 - 733. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Marriott and K. L. Bost IL-4 and IFN-{gamma} Up-Regulate Substance P Receptor Expression in Murine Peritoneal Macrophages J. Immunol., July 1, 2000; 165(1): 182 - 191. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
