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Role of TGF-ß1 on the IgE-Dependent Anaphylaxis Reaction¹

Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Chonbuk, South Korea

TGF-ß1 is a member of a family of polypeptide factors that control proliferation, differentiation, chemotaxis, and other functions in many cell types. TGF-ß1 has been shown to inhibit many immunologic functions. However, here we report that TGF-ß1 has an important role in the elicitation of IgE-dependent allergic reactions. The synthetic antisense TGF-ß1 oligonucleotides dose-dependently inhibit passive cutaneous anaphylaxis (PCA) reaction and histamine release from the mast cells activated by anti-DNP IgE in rats. The level of cAMP in mast cells, when antisense TGF-ß1 oligonucleotides was added, significantly increased 7-fold compared with that of basal cells. The antisense TGF-ß1 oligonucleotides also had a significant inhibitory effect on anti-DNP IgE-induced TNF- release from mast cells. In situ hybridization analysis showed that the PCA reaction sites treated with antisense TGF-ß1 oligonucleotides exhibited no detectable levels of TGF-ß1 and L-histidine decarboxylase mRNA after anti-DNP IgE stimulation, whereas the PCA reaction sites treated with sense TGF-ß1 oligonucleotides possessed significant amounts of their mRNA. Additionally, neutralizing Ab to TGF-ß1 blocked the PCA reaction significantly, but its Ab did not inhibit peritoneal mast cell-released histamine upon treatment with anti-DNP IgE. Our results suggest that TGF-ß1 is critical to the development of IgE-dependent anaphylaxis reactions.

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