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*
Department of Internal Medicine, Justus-Liebig-University, Giessen, Germany;
Institute of Medical Microbiology, Justus-Liebig-University, Giessen, Germany;
Institute of Microbiology, Heinrich-Heine-University, Düsseldorf, Germany; and
§
Charite, Department of Internal Medicine, Humboldt-University, Berlin, Germany
Chlamydia pneumoniae is an important respiratory
pathogen. Recently, its presence has been demonstrated in
atherosclerotic lesions. In this study, we characterized C.
pneumoniae-mediated activation of endothelial cells and
demonstrated an enhanced expression of endothelial adhesion molecules
followed by subsequent rolling, adhesion, and transmigration of
leukocytes (monocytes, granulocytes). These effects were blocked by
mAbs against endothelial and/or leukocyte adhesion molecules
(ß1 and ß2 integrins). Additionally,
activation of different signal transduction pathways in C.
pneumoniae-infected endothelial cells was shown: protein
tyrosine phosphorylation, up-regulation of phosphorylated p42/p44
mitogen-activated protein kinase, and NF-
B activation/translocation
occurred within 10–15 min. Increased mRNA and surface expression of
E-selectin, ICAM-1, and VCAM-1 were noted within hours. Thus, C.
pneumoniae triggers a cascade of events that could lead to
endothelial activation, inflammation, and thrombosis, which in turn may
result in or may promote atherosclerosis.
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S. Montigiani, F. Falugi, M. Scarselli, O. Finco, R. Petracca, G. Galli, M. Mariani, R. Manetti, M. Agnusdei, R. Cevenini, et al. Genomic Approach for Analysis of Surface Proteins in Chlamydia pneumoniae Infect. Immun., January 1, 2002; 70(1): 368 - 379. [Abstract] [Full Text] [PDF]
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K. Rajalingam, H. Al-Younes, A. Muller, T. F. Meyer, A. J. Szczepek, and T. Rudel Epithelial Cells Infected with Chlamydophila pneumoniae (Chlamydia pneumoniae) Are Resistant to Apoptosis Infect. Immun., December 1, 2001; 69(12): 7880 - 7888. [Abstract] [Full Text] [PDF]
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C. Wahl, F. Oswald, U. Simnacher, S. Weiss, R. Marre, and A. Essig Survival of Chlamydia pneumoniae-Infected Mono Mac 6 Cells Is Dependent on NF-kappa B Binding Activity Infect. Immun., November 1, 2001; 69(11): 7039 - 7045. [Abstract] [Full Text] [PDF]
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 Y.-C. Shen, C.-J. Chou, W.-F. Chiou, and C.-F. Chen Anti-Inflammatory Effects of the Partially Purified Extract of Radix Stephaniae tetrandrae: Comparative Studies of Its Active Principles Tetrandrine and Fangchinoline on Human Polymorphonuclear Leukocyte Functions Mol. Pharmacol., November 1, 2001; 60(5): 1083 - 1090. [Abstract] [Full Text]
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O. Fuhrmann, M. Arvand, A. Gohler, M. Schmid, M. Krull, S. Hippenstiel, J. Seybold, C. Dehio, and N. Suttorp Bartonella henselae Induces NF-{kappa}B-Dependent Upregulation of Adhesion Molecules in Cultured Human Endothelial Cells: Possible Role of Outer Membrane Proteins as Pathogenic Factors Infect. Immun., August 1, 2001; 69(8): 5088 - 5097. [Abstract] [Full Text] [PDF]
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B. K. Coombes and J. B. Mahony cDNA Array Analysis of Altered Gene Expression in Human Endothelial Cells in Response to Chlamydia pneumoniae Infection Infect. Immun., March 1, 2001; 69(3): 1420 - 1427. [Abstract] [Full Text] [PDF]
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N. Mozaffarian, A. Casadevall, and J. W. Berman Inhibition of Human Endothelial Cell Chemokine Production by the Opportunistic Fungal Pathogen Cryptococcus neoformans J. Immunol., August 1, 2000; 165(3): 1541 - 1547. [Abstract] [Full Text] [PDF]
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R. E. Molestina, R. D. Miller, A. B. Lentsch, J. A. Ramirez, and J. T. Summersgill Requirement for NF-kappa B in Transcriptional Activation of Monocyte Chemotactic Protein 1 by Chlamydia pneumoniae in Human Endothelial Cells Infect. Immun., July 1, 2000; 68(7): 4282 - 4288. [Abstract] [Full Text] [PDF]
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 S. Hippenstiel, S. Soeth, B. Kellas, O. Fuhrmann, J. Seybold, M. Krull, C. v. Eichel-Streiber, M. Goebeler, S. Ludwig, and N. Suttorp Rho proteins and the p38-MAPK pathway are important mediators for LPS-induced interleukin-8 expression in human endothelial cells Blood, May 15, 2000; 95(10): 3044 - 3051. [Abstract] [Full Text] [PDF]
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S. Sasu, D. LaVerda, N. Qureshi, D. T. Golenbock, and D. Beasley Chlamydia pneumoniae and Chlamydial Heat Shock Protein 60 Stimulate Proliferation of Human Vascular Smooth Muscle Cells via Toll-Like Receptor 4 and p44/p42 Mitogen-Activated Protein Kinase Activation Circ. Res., August 3, 2001; 89(3): 244 - 250. [Abstract] [Full Text] [PDF]
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