HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ma, N. Articles by Streilein, J. W. Search for Related Content PubMed PubMed Citation Articles by Ma, N. Articles by Streilein, J. W.

T Cell Immunity Induced by Allogeneic Microglia in Relation to Neuronal Retina Transplantation¹

Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA, 02114

Microglia share a lineage relationship with bone marrow-derived monocytes/macrophages and dendritic cells, and their inclusion in retinal and brain transplants may function as "passenger leukocytes." In other solid allografts, passenger leukocytes are the primary sources of immunogenicity, triggering alloimmune rejection. We have conducted a series of in vitro and in vivo studies examining the capacity of microglia cultured from forebrain to activate alloreactive T cells and to induce and elicit alloimmunity. Cultured microglia expressed class II MHC molecules and costimulatory molecules (B7-1, B7-2, and CD40), and they secreted IL-12. Cultured microglia injected s.c. into naive recipients induced allospecific delayed hypersensitivity and elicited delayed hypersensitivity directed at alloantigens. Cultured microglia differed from conventional APCs by secreting significant amounts of mature TGF-ß2, but smaller amounts of IL-12. Moreover, while both cultured microglia and conventional APC stimulated T cell proliferation in vitro, microglia directed the responding T cells toward the Th2 pathway in which IL-4, but not IL-2 and IFN-, was secreted. The abilities of microglia to secrete TGF-ß2, to stimulate alloreactive Th2 cells, and to induce anterior chamber associated immune deviation when injected into the eye of naive allogeneic mice suggest that they are not typical passenger leukocytes. The unique functional properties of cultured microglia may account for the capacity of neonatal retinal tissue transplanted into the eye to alter the systemic alloimmune response in a manner that delays, but does not prevent, graft rejection.

This article has been cited by other articles:

				N. D. Radtke, R. B. Aramant, M. J. Seiler, H. M. Petry, and D. Pidwell Vision Change After Sheet Transplant of Fetal Retina With Retinal Pigment Epithelium to a Patient With Retinitis Pigmentosa Arch Ophthalmol, August 1, 2004; 122(8): 1159 - 1165. [Abstract] [Full Text] [PDF]

				R. P. M. Sutmuller, L. R. H. M. Schurmans, L. M. van Duivenvoorde, J. A. Tine, E. I. H. van der Voort, R. E. M. Toes, C. J. M. Melief, M. J. Jager, and R. Offringa Adoptive T Cell Immunotherapy of Human Uveal Melanoma Targeting gp100 J. Immunol., December 15, 2000; 165(12): 7308 - 7315. [Abstract] [Full Text] [PDF]

				C. Broderick, L. Duncan, N. Taylor, and A. D. Dick IFN-{gamma} and LPS-Mediated IL-10-Dependent Suppression of Retinal Microglial Activation Invest. Ophthalmol. Vis. Sci., August 1, 2000; 41(9): 2613 - 2622. [Abstract] [Full Text]

				G. Tezel, M. R. Hernandez, and M. B. Wax Immunostaining of Heat Shock Proteins in the Retina and Optic Nerve Head of Normal and Glaucomatous Eyes Arch Ophthalmol, April 1, 2000; 118(4): 511 - 518. [Abstract] [Full Text] [PDF]