HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Suzuki, K. Articles by Sendo, F. Search for Related Content PubMed PubMed Citation Articles by Suzuki, K. Articles by Sendo, F.

A Novel Glycosylphosphatidyl Inositol-Anchored Protein on Human Leukocytes: A Possible Role for Regulation of Neutrophil Adherence and Migration¹

Kichiya Suzuki^*, Tadashi Watanabe^*, Shin-ichi Sakurai^*, Kazuhisa Ohtake^*, Taroh Kinoshita, Akemi Araki^*, Teizo Fujita, Hiroshi Takei^*, Yuji Takeda^*, Yukiko Sato^*, Takao Yamashita^*, Yoshihiko Araki^* and Fujiro Sendo^2,*

^* Department of Immunology and Parasitology, Yamagata University School of Medicine, Yamagata, Japan; Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Suita, Japan; and Department of Biochemistry, Fukushima Medical College, Fukushima, Japan

We report here a novel glycosylphosphatidyl-inositol (GPI)-anchored glycoprotein on human leukocytes. Treatment of neutrophils with a mAb (3H9) to this molecule sequentially up-regulates and down-regulates ß₂ integrin-dependent adhesion of these cells as well as their transendothelial migration in vitro. In addition, this mAb simultaneously modulates the avidity of ß₂ integrin for its ligand, iC3b, with kinetics similar to those observed in 3H9 modulation of neutrophil adherence. This mAb also induces ß₂ integrin-dependent cytoskeletal remodeling. This novel GPI-anchored protein (GPI-80) is highly homologous with Vanin-1, a recently reported GPI-anchored protein that is expressed on perivascular thymic stromal cells and is involved in thymus homing in mice. The finding that both GPI-80 and Vanin-1 are 40% homologous with human biotinidase suggests the existence of a biotinidase superfamily of molecules that may be involved in the regulation of leukocyte trafficking.

This article has been cited by other articles:

				R. Soleti, T. Benameur, C. Porro, M. A. Panaro, R. Andriantsitohaina, and M. C. Martinez Microparticles harboring Sonic Hedgehog promote angiogenesis through the upregulation of adhesion proteins and proangiogenic factors Carcinogenesis, April 1, 2009; 30(4): 580 - 588. [Abstract] [Full Text] [PDF]

				G. Min-Oo, A. Fortin, G. Pitari, M. Tam, M. M. Stevenson, and P. Gros Complex genetic control of susceptibility to malaria: positional cloning of the Char9 locus J. Exp. Med., March 19, 2007; 204(3): 511 - 524. [Abstract] [Full Text] [PDF]

				C. J. G. de Almeida, L. B. Chiarini, J. P. da Silva, P. M. R. e Silva, M. A. Martins, and R. Linden The cellular prion protein modulates phagocytosis and inflammatory response J. Leukoc. Biol., February 1, 2005; 77(2): 238 - 246. [Abstract] [Full Text] [PDF]

				A. Funaro, E. Ortolan, B. Ferranti, L. Gargiulo, R. Notaro, L. Luzzatto, and F. Malavasi CD157 is an important mediator of neutrophil adhesion and migration Blood, December 15, 2004; 104(13): 4269 - 4278. [Abstract] [Full Text] [PDF]

				H. Sasaki, Y. Fujii, N. Ide, and P. Starostik Chromosome 6 Abnormalities Correlated with Thymoma Progression Am. J. Pathol., December 1, 2003; 163(6): 2635 - 2636. [Full Text]

				H. Yoshitake, Y. Takeda, T. Nitto, and F. Sendo Cross-linking of GPI-80, a possible regulatory molecule of cell adhesion, induces up-regulation of CD11b/CD18 expression on neutrophil surfaces and shedding of L-selectin J. Leukoc. Biol., February 1, 2002; 71(2): 205 - 211. [Abstract] [Full Text] [PDF]

				X. Du and M. G. Low Down-Regulation of Glycosylphosphatidylinositol-Specific Phospholipase D Induced by Lipopolysaccharide and Oxidative Stress in the Murine Monocyte- Macrophage Cell Line RAW 264.7 Infect. Immun., May 1, 2001; 69(5): 3214 - 3223. [Abstract] [Full Text] [PDF]

				A. Kurita, T. Takizawa, T. Takayama, K. Totsukawa, S. Matsubara, H. Shibahara, M.-C. Orgebin-Crist, F. Sendo, Y. Shinkai, and Y. Araki Identification, Cloning, and Initial Characterization of a Novel Mouse Testicular Germ Cell-Specific Antigen Biol Reprod, March 1, 2001; 64(3): 935 - 945. [Abstract] [Full Text]

				C. Dahlgren, A. Karlsson, and F. Sendo Neutrophil secretory vesicles are the intracellular reservoir for GPI-80, a protein with adhesion-regulating potential J. Leukoc. Biol., January 1, 2001; 69(1): 57 - 62. [Abstract] [Full Text]

				Y. Nakamura-Sato, K. Sasaki, H. Watanabe, Y. Araki, and F. Sendo Clustering on the forward surfaces of migrating neutrophils of a novel GPI-anchored protein that may regulate neutrophil adherence and migration J. Leukoc. Biol., November 1, 2000; 68(5): 650 - 654. [Abstract] [Full Text]

				S. Grimmond, N. V. Hateren, P. Siggers, R. Arkell, R. Larder, M. B. Soares, M. d. F. Bonaldo, L. Smith, Z. Tymowska-Lalanne, C. Wells, et al. Sexually dimorphic expression of protease nexin-1 and vanin-1 in the developing mouse gonad prior to overt differentiation suggests a role in mammalian sexual development Hum. Mol. Genet., June 12, 2000; 9(10): 1553 - 1560. [Abstract] [Full Text] [PDF]