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Increased Generation of CD8⁺ T Cell Clones in p53 Mutant Mice¹

Xianzheng Zhou^*, Stacey Wong^*, Jurgen Walter^*, Tyler Jacks^*, and Herman N. Eisen^2,*

^* Center for Cancer Research and Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139

Very few cultured CD8⁺ T cell clones can normally be obtained from a single mouse and maintained in long-term culture. To improve the yield, we immunized p53 mutant mice with peptides of Sendai virus (FAPGNYPAL) and influenza virus (ASNENMETM) origin. Substantially more clones could be derived from p53^-/- mice than from similarly treated wild-type mice (p53^+/+); an intermediate yield was obtained from heterozygous mice (p53^+/-). CTL lines or clones from p53^-/- mice exhibited greater proliferative activity and resistance to -irradiation than those from p53^+/+ mice, and were cytolytically potent.