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Center for Cancer Research and Department of Biology,
Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139
Very few cultured CD8+ T cell clones can normally be
obtained from a single mouse and maintained in long-term culture. To
improve the yield, we immunized p53 mutant mice with peptides of Sendai
virus (FAPGNYPAL) and influenza virus (ASNENMETM) origin.
Substantially more clones could be derived from
p53-/- mice than from similarly treated wild-type
mice (p53+/+); an intermediate yield was obtained
from heterozygous mice (p53+/-). CTL lines or
clones from p53-/- mice exhibited greater proliferative
activity and resistance to
-irradiation than those from
p53+/+ mice, and were cytolytically
potent.
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