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CUTTING EDGE |
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Abteilung Immunologie, Institut für Zellbiologie, Universität Tübingen, Tübingen, Germany; and
Contrat Jeune Formation Institut National de la Santé et de la Recherche Médicale 94-03, Laboratoire d’Histocompatibilité, Etablissement de Transfusion Sanguine, Strasbourg, France
Immunization with heat shock proteins (HSPs) induces Ag-specific CTL responses. The specificity of the immune response is based on peptides associated with HSPs. To investigate how exogenous HSP/peptide complexes gain access to the MHC class I-restricted Ag presentation pathway, we incubated the monocytic cell line P388D1 and the dendritic cell line D2SC/1 with gold-labeled HSPs gp96 and HSC70. We show that HSPs bind specifically to the surface of these APCs and are internalized spontaneously by receptor-mediated endocytosis, demonstrating the existence of specific receptors for HSPs on these cells. In addition, we observe colocalization of internalized HSPs and surface MHC class I molecules in early and late endosomal structures. These findings provide possible explanations for the immunogenicity of HSP/peptide complexes and for the transfer of HSP-associated peptides onto MHC class I molecules.
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N. Hilf, H. Singh-Jasuja, P. Schwarzmaier, C. Gouttefangeas, H.-G. Rammensee, and H. Schild Human platelets express heat shock protein receptors and regulate dendritic cell maturation Blood, May 15, 2002; 99(10): 3676 - 3682. [Abstract] [Full Text] [PDF]
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J. Robert, J. Gantress, L. Rau, A. Bell, and N. Cohen Minor Histocompatibility Antigen-Specific MHC-Restricted CD8 T Cell Responses Elicited by Heat Shock Proteins J. Immunol., February 15, 2002; 168(4): 1697 - 1703. [Abstract] [Full Text] [PDF]
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A.-M. Sponaas, U. Zuegel, S. Weber, R. Hurwitz, R. Winter, S. Lamer, P. R. Jungblut, and S. H. E. Kaufmann Immunization with gp96 from Listeria monocytogenes-Infected Mice Is Due to N-Formylated Listerial Peptides J. Immunol., December 1, 2001; 167(11): 6480 - 6486. [Abstract] [Full Text] [PDF]
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S. Somersan, M. Larsson, J. F. Fonteneau, S. Basu, P. Srivastava, and N. Bhardwaj Primary Tumor Tissue Lysates Are Enriched in Heat Shock Proteins and Induce the Maturation of Human Dendritic Cells J. Immunol., November 1, 2001; 167(9): 4844 - 4852. [Abstract] [Full Text] [PDF]
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M. d. C. Salamone, A. K. Mendiguren, G. V. Salamone, and L. Fainboim Membrane trafficking of CD1c on activated T cells J. Leukoc. Biol., October 1, 2001; 70(4): 567 - 577. [Abstract] [Full Text] [PDF]
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 U. Zugel, A.-M. Sponaas, J. Neckermann, B. Schoel, and S. H. E. Kaufmann gp96-Peptide Vaccination of Mice against Intracellular Bacteria Infect. Immun., June 1, 2001; 69(6): 4164 - 4167. [Abstract] [Full Text] [PDF]
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Z. Tabi, M. Moutaftsi, and L. K. Borysiewicz Human Cytomegalovirus pp65- and Immediate Early 1 Antigen-Specific HLA Class I-Restricted Cytotoxic T Cell Responses Induced by Cross-Presentation of Viral Antigens J. Immunol., May 1, 2001; 166(9): 5695 - 5703. [Abstract] [Full Text] [PDF]
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 P. A. MacAry, M. Lindsay, M. A. Scott, J. I. O. Craig, J. P. Luzio, and P. J. Lehner Mobilization of MHC class I molecules from late endosomes to the cell surface following activation of CD34-derived human Langerhans cells PNAS, March 27, 2001; 98(7): 3982 - 3987. [Abstract] [Full Text] [PDF]
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X.-Y. Wang, L. Kazim, E. A. Repasky, and J. R. Subjeck Characterization of Heat Shock Protein 110 and Glucose-Regulated Protein 170 as Cancer Vaccines and the Effect of Fever-Range Hyperthermia on Vaccine Activity J. Immunol., January 1, 2001; 166(1): 490 - 497. [Abstract] [Full Text] [PDF]
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C. Maranon, L. Planelles, C. Alonso, and M. C. Lopez HSP70 from Trypanosoma cruzi is endowed with specific cell proliferation potential leading to apoptosis Int. Immunol., December 1, 2000; 12(12): 1685 - 1693. [Abstract] [Full Text] [PDF]
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R. J. Binder, M. L. Harris, A. Menoret, and P. K. Srivastava Saturation, Competition, and Specificity in Interaction of Heat Shock Proteins (hsp) gp96, hsp90, and hsp70 with CD11b+ Cells J. Immunol., September 1, 2000; 165(5): 2582 - 2587. [Abstract] [Full Text] [PDF]
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D. Arnold-Schild, C. Kleist, M. Welschof, G. Opelz, H.-G. Rammensee, H. Schild, and P. Terness One-Step Single-Chain Fv Recombinant Antibody-based Purification of gp96 for Vaccine Development Cancer Res., August 1, 2000; 60(15): 4175 - 4178. [Abstract] [Full Text]
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M. Lanuti, S. Rudginsky, S. D. Force, E. S. Lambright, W. M. Siders, M. Y. Chang, K. M. Amin, L. R. Kaiser, R. K. Scheule, and S. M. Albelda Cationic Lipid:Bacterial DNA Complexes Elicit Adaptive Cellular Immunity in Murine Intraperitoneal Tumor Models Cancer Res., June 1, 2000; 60(11): 2955 - 2963. [Abstract] [Full Text] [PDF]
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M. Graner, A. Raymond, D. Romney, L. He, L. Whitesell, and E. Katsanis Immunoprotective Activities of Multiple Chaperone Proteins Isolated from Murine B-Cell Leukemia/Lymphoma Clin. Cancer Res., March 1, 2000; 6(3): 909 - 915. [Abstract] [Full Text]
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N. A. Linderoth, A. Popowicz, and S. Sastry Identification of the Peptide-binding Site in the Heat Shock Chaperone/Tumor Rejection Antigen gp96 (Grp94) J. Biol. Chem., February 25, 2000; 275(8): 5472 - 5477. [Abstract] [Full Text] [PDF]
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C.-H. Chen, T.-L. Wang, C.-F. Hung, Y. Yang, R. A. Young, D. M. Pardoll, and T-C. Wu Enhancement of DNA Vaccine Potency by Linkage of Antigen Gene to an HSP70 Gene Cancer Res., February 1, 2000; 60(4): 1035 - 1042. [Abstract] [Full Text]
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C. Thery, A. Regnault, J. Garin, J. Wolfers, L. Zitvogel, P. Ricciardi-Castagnoli, G. Raposo, and S. Amigorena Molecular Characterization of Dendritic Cell-derived Exosomes: Selective Accumulation of the Heat Shock Protein hsc73 J. Cell Biol., November 1, 1999; 147(3): 599 - 610. [Abstract] [Full Text] [PDF]
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F. Castellino, P. E. Boucher, K. Eichelberg, M. Mayhew, J. E. Rothman, A. N. Houghton, and R. N. Germain Receptor-mediated Uptake of Antigen/Heat Shock Protein Complexes Results in Major Histocompatibility Complex Class I Antigen Presentation via Two Distinct Processing Pathways J. Exp. Med., June 6, 1999; 191(11): 1957 - 1964. [Abstract] [Full Text] [PDF]
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H. Singh-Jasuja, R. E.M. Toes, P. Spee, C. Munz, N. Hilf, S. P. Schoenberger, P. Ricciardi-Castagnoli, J. Neefjes, H.-G. Rammensee, D. Arnold-Schild, et al. Cross-Presentation of Glycoprotein 96-associated Antigens on Major Histocompatibility Complex Class I Molecules Requires Receptor-mediated Endocytosis J. Exp. Med., June 6, 1999; 191(11): 1965 - 1974. [Abstract] [Full Text] [PDF]
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J. Wassenberg, C Dezfulian, and C. Nicchitta Receptor mediated and fluid phase pathways for internalization of the ER Hsp90 chaperone GRP94 in murine macrophages J. Cell Sci., January 7, 1999; 112(13): 2167 - 2175. [Abstract] [PDF]
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D. Mamelak and C. Lingwood The ATPase Domain of hsp70 Possesses a Unique Binding Specificity for 3'-Sulfogalactolipids J. Biol. Chem., January 5, 2001; 276(1): 449 - 456. [Abstract] [Full Text] [PDF]
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N. A. Linderoth, M. N. Simon, J. F. Hainfeld, and S. Sastry Binding of Antigenic Peptide to the Endoplasmic Reticulum-resident Protein gp96/GRP94 Heat Shock Chaperone Occurs in Higher Order Complexes. ESSENTIAL ROLE OF SOME AROMATIC AMINO ACID RESIDUES IN THE PEPTIDE-BINDING SITE J. Biol. Chem., March 30, 2001; 276(14): 11049 - 11054. [Abstract] [Full Text] [PDF]
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F. Le Naour, L. Hohenkirk, A. Grolleau, D. E. Misek, P. Lescure, J. D. Geiger, S. Hanash, and L. Beretta Profiling Changes in Gene Expression during Differentiation and Maturation of Monocyte-derived Dendritic Cells Using Both Oligonucleotide Microarrays and Proteomics J. Biol. Chem., May 18, 2001; 276(21): 17920 - 17931. [Abstract] [Full Text] [PDF]
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R. M. Vabulas, P. Ahmad-Nejad, C. da Costa, T. Miethke, C. J. Kirschning, H. Hacker, and H. Wagner Endocytosed HSP60s Use Toll-like Receptor 2 (TLR2) and TLR4 to Activate the Toll/Interleukin-1 Receptor Signaling Pathway in Innate Immune Cells J. Biol. Chem., August 10, 2001; 276(33): 31332 - 31339. [Abstract] [Full Text] [PDF]
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