| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Clincial Immunology Section, Laboratory of Clinical Investigation, and
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, and
Molecular Immunogenetics and Vaccine Research Section, Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892; and
§
Immunex Corporation, Seattle, WA 98101
The role of exogenous stimulation of CD40 by CD40 ligand (CD40L) in
dendritic cell (DC) maturation, CC-chemokine production, and CCR5
receptor expression was examined using a soluble trimeric CD40L
agonist protein (CD40LT). Stimulation of monocyte-derived DCs with
CD40LT enhanced the production of the CC-chemokines macrophage
inflammatory protein (MIP)-1
, MIP-1ß, and RANTES and diminished
surface expression of CCR5. Based on these findings, the functional
role of CD40LT stimulation on the ability of DCs to replicate and
transmit HIV viral infection was studied. The addition of CD40LT to
cocultures of naive CD4+ T cells and autologous DCs (T/DC)
infected with the macrophage-tropic isolate, HIVBaL, caused
a striking reduction in reverse transcriptase (RT) activity after 10
and 14 days of culture. The addition of a mixture of Abs against
CC-chemokines abrogated the decrease in RT activity, demonstrating that
the inhibitory effect mediated by CD40LT was CC-chemokine-dependent. In
contrast, the presence of CD40LT in T/DC cocultures infected with the T
cell-tropic isolate, HIVIIIB, caused an increase in RT
activity that was CC-chemokine-independent. Of note, CD40LT stimulation
also inhibited RT activity in cultures containing macrophage-tropic
virus (HIVBaL)-infected DC only. However, in contrast to
the results seen in the T/DC cocultures, CD40LT stimulation inhibited
RT activity in cultures of DCs alone in a CC-chemokine-independent
manner. Together, these results show that CD40LT stimulation of DCs
suppresses HIV replication and transmission to CD4+ T cells
by two potentially different mechanisms.
This article has been cited by other articles:
|
|
 |
|
  C. Dong, A. M. Janas, J.-H. Wang, W. J. Olson, and L. Wu Characterization of Human Immunodeficiency Virus Type 1 Replication in Immature and Mature Dendritic Cells Reveals Dissociable cis- and trans-Infection J. Virol., October 15, 2007; 81(20): 11352 - 11362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. Kornbluth and G. W. Stone Immunostimulatory combinations: designing the next generation of vaccine adjuvants J. Leukoc. Biol., November 1, 2006; 80(5): 1084 - 1102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Ancuta, P. Autissier, A. Wurcel, T. Zaman, D. Stone, and D. Gabuzda CD16+ Monocyte-Derived Macrophages Activate Resting T Cells for HIV Infection by Producing CCR3 and CCR4 Ligands J. Immunol., May 15, 2006; 176(10): 5760 - 5771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Granelli-Piperno, I. Shimeliovich, M. Pack, C. Trumpfheller, and R. M. Steinman HIV-1 Selectively Infects a Subset of Nonmaturing BDCA1-Positive Dendritic Cells in Human Blood J. Immunol., January 15, 2006; 176(2): 991 - 998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. L. Hodara, M. C. Velasquillo, L. M. Parodi, and L. D. Giavedoni Expression of CD154 by a Simian Immunodeficiency Virus Vector Induces Only Transitory Changes in Rhesus Macaques J. Virol., April 15, 2005; 79(8): 4679 - 4690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Chougnet Role of CD40 Ligand dysregulation in HIV-associated dysfunction of antigen-presenting cells J. Leukoc. Biol., November 1, 2003; 74(5): 702 - 709. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Messmer, B. Messmer, and N. Chiorazzi The global transcriptional maturation program and stimuli-specific gene expression profiles of human myeloid dendritic cells Int. Immunol., April 1, 2003; 15(4): 491 - 503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Fong, M. Mengozzi, N. W. Abbey, B. G. Herndier, and E. G. Engleman Productive Infection of Plasmacytoid Dendritic Cells with Human Immunodeficiency Virus Type 1 Is Triggered by CD40 Ligation J. Virol., October 2, 2002; 76(21): 11033 - 11041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Frank, M. Piatak Jr., H. Stoessel, N. Romani, D. Bonnyay, J.D. Lifson, and M. Pope Infectious and Whole Inactivated Simian Immunodeficiency Viruses Interact Similarly with Primate Dendritic Cells (DCs): Differential Intracellular Fate of Virions in Mature and Immature DCs J. Virol., February 22, 2002; 76(6): 2936 - 2951. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-Q. Zhao, X.-L. Huang, P. Gupta, L. Borowski, Z. Fan, S. C. Watkins, E. K. Thomas, and C. R. Rinaldo Jr. Induction of Anti-Human Immunodeficiency Virus Type 1 (HIV-1) CD8+ and CD4+ T-Cell Reactivity by Dendritic Cells Loaded with HIV-1 X4-Infected Apoptotic Cells J. Virol., February 22, 2002; 76(6): 3007 - 3014. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Cotter, J. Zheng, M. Che, D. Niemann, Y. Liu, J. He, E. Thomas, and H. E. Gendelman Regulation of Human Immunodeficiency Virus Type 1 Infection, {beta}-Chemokine Production, and CCR5 Expression in CD40L-Stimulated Macrophages: Immune Control of Viral Entry J. Virol., May 1, 2001; 75(9): 4308 - 4320. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Bakri, C. Schiffer, V. Zennou, P. Charneau, E. Kahn, A. Benjouad, J. C. Gluckman, and B. Canque The Maturation of Dendritic Cells Results in Postintegration Inhibition of HIV-1 Replication J. Immunol., March 15, 2001; 166(6): 3780 - 3788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Chougnet, C. Freitag, M. Schito, E. K. Thomas, A. Sher, and G. M. Shearer In Vivo CD40-CD154 (CD40 Ligand) Interaction Induces Integrated HIV Expression by APC in an HIV-1-Transgenic Mouse Model J. Immunol., March 1, 2001; 166(5): 3210 - 3217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Howard and S. D. Miller Autoimmune Intervention by CD154 Blockade Prevents T Cell Retention and Effector Function in the Target Organ J. Immunol., February 1, 2001; 166(3): 1547 - 1553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Kornbluth The emerging role of CD40 ligand in HIV infection J. Leukoc. Biol., September 1, 2000; 68(3): 373 - 382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Jourdan, J.-P. Vendrell, M.-F. Huguet, M. Segondy, J. Bousquet, J. Pene, and H. Yssel Cytokines and Cell Surface Molecules Independently Induce CXCR4 Expression on CD4+ CCR7+ Human Memory T Cells J. Immunol., July 15, 2000; 165(2): 716 - 724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Hornung, G. Scala, and M. J. Lenardo TNF-{alpha}-Induced Secretion of C-C Chemokines Modulates C-C Chemokine Receptor 5 Expression on Peripheral Blood Lymphocytes J. Immunol., June 15, 2000; 164(12): 6180 - 6187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Borges, R. E. Miller, J. Jones, K. Ariail, J. Whitmore, W. Fanslow, and D. H. Lynch Synergistic Action of fms-Like Tyrosine Kinase 3 Ligand and CD40 Ligand in the Induction of Dendritic Cells and Generation of Antitumor Immunity In Vivo J. Immunol., August 1, 1999; 163(3): 1289 - 1297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Chougnet, S. S. Cohen, T. Kawamura, A. L. Landay, H. A. Kessler, E. Thomas, A. Blauvelt, and G. M. Shearer Normal Immune Function of Monocyte-Derived Dendritic Cells from HIV-Infected Individuals: Implications for Immunotherapy J. Immunol., August 1, 1999; 163(3): 1666 - 1673. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
