HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Agadjanyan, M. G. Articles by Weiner, D. B. Search for Related Content PubMed PubMed Citation Articles by Agadjanyan, M. G. Articles by Weiner, D. B.

CD86 (B7-2) Can Function to Drive MHC-Restricted Antigen-Specific CTL Responses In Vivo¹

Michael G. Agadjanyan^2,3,*,§, Jong J. Kim^2,*, Neil Trivedi^*, Darren M. Wilson^*, Behjatolah Monzavi-Karbassi^*, Lake D. Morrison, Liesl K. Nottingham, Tzvete Dentchev^*, Anthony Tsai^*, Kesen Dang^*, Ara A. Chalian, Michael A. Maldonado, William V. Williams and David B. Weiner^*

Departments of ^* Pathology and Laboratory Medicine, Medicine, and Otolaryngology, University of Pennsylvania, Philadelphia, PA 19104; and ^§ Institute of Viral Preparation, Russian Academy of Medicine, Moscow, Russia

Activation of T cells requires both TCR-specific ligation by direct contact with peptide Ag-MHC complexes and coligation of the B7 family of ligands through CD28/CTLA-4 on the T cell surface. We recently reported that coadministration of CD86 cDNA along with DNA encoding HIV-1 Ags i.m. dramatically increased Ag-specific CTL responses. We investigated whether the bone marrow-derived professional APCs or muscle cells were responsible for the enhancement of CTL responses following CD86 coadministration. Accordingly, we analyzed CTL induction in bone marrow chimeras. These chimeras are capable of generating functional viral-specific CTLs against vaccinia virus and therefore represent a useful model system to study APC/T cell function in vivo. In vaccinated chimeras, we observed that only CD86 + Ag + MHC class I results in 1) detectable CTLs following in vitro restimulation, 2) detectable direct CTLs, 3) enhanced IFN- production in an Ag-specific manner, and 4) dramatic tissue invasion of T cells. These results support that CD86 plays a central role in CTL induction in vivo, enabling non-bone marrow-derived cells to prime CTLs, a property previously associated solely with bone marrow-derived APCs.

This article has been cited by other articles:

				S. Radhakrishnan, L. T. Nguyen, B. Ciric, D. Flies, V. P. V. Keulen, K. Tamada, L. Chen, M. Rodriguez, and L. R. Pease Immunotherapeutic Potential of B7-DC (PD-L2) Cross-Linking Antibody In Conferring Antitumor Immunity Cancer Res., July 15, 2004; 64(14): 4965 - 4972. [Abstract] [Full Text] [PDF]

				M. Giri, K. E. Ugen, and D. B. Weiner DNA Vaccines against Human Immunodeficiency Virus Type 1 in the Past Decade Clin. Microbiol. Rev., April 1, 2004; 17(2): 370 - 389. [Abstract] [Full Text] [PDF]

				M. G. Agadjanyan, M. A. Chattergoon, M. J. Holterman, B. Monzavi-Karbassi, J. J. Kim, T. Dentchev, D. Wilson, V. Ayyavoo, L. J. Montaner, T. Kieber-Emmons, et al. Costimulatory Molecule Immune Enhancement in a Plasmid Vaccine Model Is Regulated in Part Through the Ig Constant-Like Domain of CD80/86 J. Immunol., October 15, 2003; 171(8): 4311 - 4319. [Abstract] [Full Text] [PDF]

				V. V. Parekh, D. V. R. Prasad, P. P. Banerjee, B. N. Joshi, A. Kumar, and G. C. Mishra B Cells Activated by Lipopolysaccharide, But Not By Anti-Ig and Anti-CD40 Antibody, Induce Anergy in CD8+ T Cells: Role of TGF-{beta}1 J. Immunol., June 15, 2003; 170(12): 5897 - 5911. [Abstract] [Full Text] [PDF]

				C. Vasu, A. Wang, S. R. Gorla, S. Kaithamana, B. S. Prabhakar, and M. J. Holterman CD80 and CD86 C domains play an important role in receptor binding and co-stimulatory properties Int. Immunol., February 1, 2003; 15(2): 167 - 175. [Abstract] [Full Text] [PDF]

				X. Shen, S. B. J. Wong, C. B. Buck, J. Zhang, and R. F. Siliciano Direct Priming and Cross-Priming Contribute Differentially to the Induction of CD8+ CTL Following Exposure to Vaccinia Virus Via Different Routes J. Immunol., October 15, 2002; 169(8): 4222 - 4229. [Abstract] [Full Text] [PDF]

				S. Mercier, H. Gahery-Segard, M. Monteil, R. Lengagne, J.-G. Guillet, M. Eloit, and C. Denesvre Distinct Roles of Adenovirus Vector-Transduced Dendritic Cells, Myoblasts, and Endothelial Cells in Mediating an Immune Response against a Transgene Product J. Virol., February 22, 2002; 76(6): 2899 - 2911. [Abstract] [Full Text] [PDF]

				V. Ayyavoo, K. Muthumani, S. Kudchodkar, D. Zhang, P. Ramanathan, N. S. Dayes, J. J. Kim, J.-I. Sin, L. J. Montaner, and D. B. Weiner HIV-1 viral protein R compromises cellular immune function in vivo Int. Immunol., January 1, 2002; 14(1): 13 - 22. [Abstract] [Full Text] [PDF]

				K. Chan, D. J. Lee, A. Schubert, C. M. Tang, B. Crain, S. P. Schoenberger, and M. Corr The Roles of MHC Class II, CD40, and B7 Costimulation in CTL Induction by Plasmid DNA J. Immunol., March 1, 2001; 166(5): 3061 - 3066. [Abstract] [Full Text] [PDF]

				D. J. Shedlock and D. B. Weiner DNA vaccination: antigen presentation and the induction of immunity J. Leukoc. Biol., December 1, 2000; 68(6): 793 - 806. [Abstract] [Full Text]

				T. Maeda, M. Towatari, H. Kosugi, and H. Saito Up-regulation of costimulatory/adhesion molecules by histone deacetylase inhibitors in acute myeloid leukemia cells Blood, December 1, 2000; 96(12): 3847 - 3856. [Abstract] [Full Text] [PDF]